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Immunphänotypische Charakterisierung von Speicheldrüsentumoren unter Verwendung von Gewebe-Micro-Arrays

Immunohistochemic characterization of salivary gland tumors with tissue micro-arrays

  • Schwerpunkt: Myoepitheliale Tumoren
  • Published:
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Zusammenfassung

Systematische Analysen zur Gen- und Proteinexpression bei Speicheldrüsentumoren sind Voraussetzung für die Identifizierung tumorassoziierter Gene. Aus dem Hamburger Speicheldrüsenregister standen Proben in ausreichender Menge und Vielfalt zur Verfügung, sodass Gewebemikroarrays („tissue micro-array“/TMA) hergestellt werden konnten. Ziel war es, zu untersuchen, inwieweit TMA für die Speicheldrüsentumordiagnostik und -forschung einsetzbar sind. In der Studie wurden überwiegend Antikörper eingesetzt, die auch routinemäßig zur Absicherung der Diagnose verwendet werden.

Epitheliale Marker ließen keine tumortypspezifische Charakterisierung zu. Myoepitheliale Marker eigneten sich dagegen zur Abgrenzung biphasischer Tumoren von rein epithelialen Tumoren. Der Amylasenachweis in Azinuszellkarzinomen sowie der Nachweis von Steroidrezeptoren in diesen und anderen Speicheldrüsentumoren haben in Verbindung mit der Expression von Transkriptionsfaktoren, Tumormarkern und der Proliferationsrate bei malignen Speicheldrüsentumoren Expressionsprofile ergeben, die diagnostisch und klinisch relevant sein können und/oder eine Grundlage für weiterführende, insbesondere molekulare Untersuchungen sind.

Abstract

Systematic analysis of gene expression in salivary gland tumors is necessary to identify genes associated with specific tumor types. From the salivary gland register of the University Hospital Hamburg-Eppendorf sufficient samples of various tumors were available to generate Tissue Micro-Arrays (TMA). In light of the considerable heterogeneity of salivary gland tumors, this study was aimed at evaluating the suitability of TMA in salivary gland diagnostics and research.

Epithelial antigens are not sufficient for a tumortype-specific characterization. Myoepithelial markers are suitable for distinguishing biphasic tumor types from purely epithelial tumors. The detection of amylase in acinic cell carcinomas, and the detection of steroid hormone receptors in these and other malignant salivary gland tumors particularly in combination with the expression of transcription factors, oncogenes and proliferation associated antigens result in characteristic expression profiles. These may prove to be valuable for further investigations, especially on the molecular level.

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Danksagung

Die Arbeiten wurden durch Zuwendungen der Hamburger Stiftung zur Förderung der Krebsbekämpfung (157), der Deutschen Forschungsgemeinschaft (RO 2081/1) und der Medizinischen Fakultät Münster (IMF HU120115) gefördert.

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Authors and Affiliations

  1. Speicheldrüsenregister, Institut für Oralpathologie, Universitätsklinikum Hamburg-Eppendorf

    K. Röser, K. T. Jäkel &  T. Löning

  2. Gerhard-Domagk-Institut für Pathologie, Universitätsklinikum Münster

    H. Herbst

  3. Institut für Oralpathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistraße 52, 20246, Hamburg

    T. Löning

Authors
  1. K. Röser
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  2. K. T. Jäkel
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  3. H. Herbst
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  4. T. Löning
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Correspondence to T. Löning.

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Röser, K., Jäkel, K.T., Herbst, H. et al. Immunphänotypische Charakterisierung von Speicheldrüsentumoren unter Verwendung von Gewebe-Micro-Arrays. Pathologe 26, 345–352 (2005). https://doi.org/10.1007/s00292-005-0777-y

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  • DOI: https://doi.org/10.1007/s00292-005-0777-y

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