Some roles of methanol-soluble fraction of rubber from Manihot glaziovii. Part 1. Sitosterol and fatty acids

Summary

Manihot glaziovii rubber was dissolved in purified chloroform and coagulated with methanol. An oily like material (ACM) remaining from the coagulation process was isolated and submitted to column chromatography over silica gel yielding several fractions. The components of the major fraction, denoted collectively as F-GH, were isolated and characterised by FTIR, 1H and ¹³C NMR spectroscopies and GC/MS as sitosterol, stigmasterol and a mixture of palmitic and oleic acid. The effect of ACM itself and the components of F-GH on the thermal oxidation of rubber film at 140 °C was verified by the use of FTIR. The induction time and the rate constant were determined.