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Chitosan PVA and Acalypha indica-based nanoformulation for antimicrobial activity against Staphylococcus aureus

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Abstract

Antimicrobial resistance is one of the major health problems associated with patient care. In this context, researchers are interested in developing novel, effective and sustainable therapeutic nanoformulations. It has been observed that chitosan, polyvinyl alcohol, copper oxide and Acalypha indica (AI) extract have proven antimicrobial efficacy either individually or in combination. The presented study was focused on development of chitosan/PVA/CuO-AI nanocomposite for antimicrobial activity. In addition, hemolytic activity of the nanocomposite was also determined. Scanning electron microscopy, Fourier-transform infrared spectroscopy and X-ray crystallography confirmed the preparation of the nanocomposite. In addition, the chitosan/PVA/CuO-AI nanocomposite demonstrated effective antimicrobial activity and it was not hemolytic on human blood samples. Moreover, the cytotoxicity assay performed on Danio rerio gill cell line recorded 85% cells viability and no significant morphological alterations were observed.

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References

  1. Afshari M, Derakhshandeh K, Hosseinzadeh L (2014) Characterisation, cytotoxicity and apoptosis studies of methotrexate-loaded PLGA and PLGA-PEG nanoparticles. J Microencapsul 31(3):239–245

    CAS  PubMed  Google Scholar 

  2. Al-hakimi AN, Asnag G, Alminderej F, Alhagri IA, Al-Hazmy SM, Abdallah E (2022) Enhanced structural, optical, electrical properties and antibacterial activity of selenium nanoparticles loaded PVA/CMC blend for electrochemical batteries and food packaging applications. Polym Test 116:107794

    CAS  Google Scholar 

  3. Atri A, Echabaane M, Bouzidi A, Harabi I, Soucase BM, Chaâbane RB (2023) Green synthesis of copper oxide nanoparticles using Ephedra Alata plant extract and a study of their antifungal, antibacterial activity and photocatalytic performance under sunlight. Heliyon 9(2):e13484

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Bagheri-Josheghani S, Bakhshi B (2022) Investigation of the antibacterial and antibiofilm activity of selenium nanoparticles against Vibrio cholerae as a potent therapeutics. Can J Infect Dis Med Microbiol 2022:3432235

    PubMed  PubMed Central  Google Scholar 

  5. Bandatang N, Pongsomboon S-A, Jumpapaeng P, Suwanakood P, Saengsuwan S (2021) Antimicrobial electrospun nanofiber mats of NaOH-hydrolyzed chitosan (HCS)/PVP/PVA incorporated with in-situ synthesized AgNPs: Fabrication, characterization, and antibacterial activity. Int J Biol Macromol 190:585–600

    CAS  PubMed  Google Scholar 

  6. Bano I, Arshad M, Yasin T, Ghauri MA, Younus M (2017) Chitosan: a potential biopolymer for wound management. Int J Biol Macromol 102:380–383

    CAS  PubMed  Google Scholar 

  7. Borkow G, Gabbay J, Dardik R, Eidelman AI, Lavie Y, Grunfeld Y, Ikher S, Huszar M, Zatcoff RC, Marikovsky M (2010) Molecular mechanisms of enhanced wound healing by copper oxide-impregnated dressings. Wound Repair Regen 18(2):266–275

    PubMed  Google Scholar 

  8. Boucher HW, Corey GR (2008) Epidemiology of methicillin-resistant Staphylococcus aureus. Clin Infect Dis 46(Suppl 5):S344-349

    PubMed  Google Scholar 

  9. Canbaz GT, Açıkel Ü, Açıkel YS (2023) Removal of heavy metal by using green synthesis ZnO NPs and ZnO-HNT composite. Biomass Convers Biorefinery: 1–16

  10. Chandrasekar S, Vijayakumar S, Rajendran R, Rajesh R, Elayarajah B (2013) Herbal-chitosan nanocomposites for durable antibacterial finishing on cotton materials. Int J Biopharm 4(3):219–224

    Google Scholar 

  11. Croisier F, Jérôme C (2013) Chitosan-based biomaterials for tissue engineering. Eur Polym J 49(4):780–792

    CAS  Google Scholar 

  12. Dardeer HM, Abbas SA, El-Sayyad GS, Ali MF (2022) Effect of titanium dioxide nanoparticles and β-cyclodextrin polymer on physicochemical, antimicrobial, and antibiofilm properties of a novel chitosan-camphor polymer. Int J Biol Macromol 219:1062–1079

    CAS  PubMed  Google Scholar 

  13. El Gohary HG, Alhagri IA, Qahtan TF, Al-Hakimi AN, Saeed A, Abolaban F, Alshammari EM, Asnag G (2023) Reinforcement of structural, thermal and electrical properties and antibacterial activity of PVA/SA blend filled with hybrid nanoparticles (Ag and TiO2 NPs): Nanodielectric for energy storage and food packaging industries. Ceram Int 49(12):20174–20184

    Google Scholar 

  14. Elabbasy MT, Alshammari MH, Zrieq R, El Bayomi RM, Tahoun AB, El-Morsy M, Abd El-Kader M (2023) Physical and biological changes of copper oxide and hydroxyapatite filled in polycaprolactone scaffolds: cellular growth behavior and antibacterial activity. J Mech Behav Biomed Mater 144:105927

    CAS  PubMed  Google Scholar 

  15. Ganeshkumar M, Ponrasu T, Krithika R, Iyappan K, Gayathri VS, Suguna L (2012) Topical application of Acalypha indica accelerates rat cutaneous wound healing by up-regulating the expression of Type I and III collagen. J Ethnopharmacol 142(1):14–22

    CAS  PubMed  Google Scholar 

  16. Gopal A, Kant V, Gopalakrishnan A, Tandan SK, Kumar D (2014) Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats. Eur J Pharmacol 731:8–19

    CAS  PubMed  Google Scholar 

  17. Gopinath K, Gnanasekar S, Al-Ghanim KA, Nicoletti M, Govindarajan M, Arumugam A, Balalakshmi C, Thanakkasaranee S (2023) Fabrication of neodymium (Nd), cadmium (Cd) and Nd: Cd doped hybrid copper oxide nanocomposites: evaluation of their antibacterial activity and cytotoxicity against human L132 cell line. Ceram Int 49(18):29933–29947

    CAS  Google Scholar 

  18. Guo Q, Ren C-W, Cai J-H, Zhang C-Y, Li Y-T, Xu B, Farooq MA (2022) The synergistic inhibition and mechanism of epicatechin gallate and Chitosan against Methicillin-resistant Staphylococcus aureus and the application in pork preservation. LWT 163:113575

    CAS  Google Scholar 

  19. Hezari S, Olad A, Dilmaghani A (2022) Modified gelatin/iron-based metal-organic framework nanocomposite hydrogel as wound dressing: synthesis, antibacterial activity, and Camellia sinensis release. Int J Biol Macromol 218:488–505

    CAS  PubMed  Google Scholar 

  20. Iacob A-T, Drăgan M, Ghețu N, Pieptu D, Vasile C, Buron F, Routier S, Giusca SE, Caruntu I-D, Profire L (2018) Preparation, characterization and wound healing effects of new membranes based on chitosan, hyaluronic acid and arginine derivatives. Polymers 10(6):607

    PubMed  PubMed Central  Google Scholar 

  21. Iqbal J, Abbasi BA, Mahmood T, Hameed S, Munir A, Kanwal S (2019) Green synthesis and characterizations of Nickel oxide nanoparticles using leaf extract of Rhamnus virgata and their potential biological applications. Appl Organomet Chem 33(8):e4950

    Google Scholar 

  22. Jannesari M, Varshosaz J, Morshed M, Zamani M (2011) Composite poly(vinyl alcohol)/poly(vinyl acetate) electrospun nanofibrous mats as a novel wound dressing matrix for controlled release of drugs. Int J Nanomed 6:993–1003

    CAS  Google Scholar 

  23. Jawad KH, Hasoon BA, Ismail RA, Shaker SS (2022) Preparation of copper oxide nanosheets by pulsed laser ablation in liquid for anticancer, antioxidant, and antibacterial activities. J Indian Chem Soc 99(11):100773

    CAS  Google Scholar 

  24. Jenifer P, Kalachaveedu M, Viswanathan A, Gnanamani A, Mubeena (2018) Fabricated approach for an effective wound dressing material based on a natural gum impregnated with Acalypha indica extract. J Bioact Compat Polym 33(6):612–628

    CAS  Google Scholar 

  25. Jiao X, Gutha Y, Zhang W (2017) Application of chitosan/poly(vinyl alcohol)/CuO (CS/PVA/CuO) beads as an adsorbent material for the removal of Pb(II) from aqueous environment. Colloids Surf, B 149:184–195

    CAS  Google Scholar 

  26. Kamarajan G, Anburaj DB, Porkalai V, Muthuvel A, Nedunchezhian G (2022) Green synthesis of ZnO nanoparticles using Acalypha indica leaf extract and their photocatalyst degradation and antibacterial activity. J Indian Chem Soc 99(10):100695

    CAS  Google Scholar 

  27. Karthik S, Suriyaprabha R, Vinoth M, Srither S, Manivasakan P, Rajendran V, Valiyaveettil S (2017) Larvicidal, super hydrophobic and antibacterial properties of herbal nanoparticles from Acalypha indica for biomedical applications. RSC Adv 7(66):41763–41770

    CAS  Google Scholar 

  28. Khatoon UT, Velidandi A, Rao GN (2023) Copper oxide nanoparticles: synthesis via chemical reduction, characterization, antibacterial activity, and possible mechanism involved. Inorg Chem Commun 149:110372

    CAS  Google Scholar 

  29. Kim JO, Park JK, Kim JH, Jin SG, Yong CS, Li DX, Choi JY, Woo JS, Yoo BK, Lyoo WS (2008) Development of polyvinyl alcohol–sodium alginate gel-matrix-based wound dressing system containing nitrofurazone. Int J Pharm 359(1–2):79–86

    CAS  PubMed  Google Scholar 

  30. Kujath P, Michelsen A (2008) Wounds–from physiology to wound dressing. Dtsch Arztebl Int 105(13):239

    PubMed  PubMed Central  Google Scholar 

  31. Kumar S, Krishnakumar B, Sobral AJ, Koh J (2019) Bio-based (chitosan/PVA/ZnO) nanocomposites film: thermally stable and photoluminescence material for removal of organic dye. Carbohyd Polym 205:559–564

    CAS  Google Scholar 

  32. Kumar V, Sharma N, Janghu P, Pasrija R, Umesh M, Chakraborty P, Sarojini S, Thomas J (2023) Synthesis and characterization of chitosan nanofibers for wound healing and drug delivery application. J Drug Deliv Sci Technol 87:104858

    CAS  Google Scholar 

  33. Lakkaboyana SK, Soontarapa K, Marella RK, Kannan K (2021) Preparation of novel chitosan polymeric nanocomposite as an efficient material for the removal of Acid Blue 25 from aqueous environment. Int J Biol Macromol 168:760–768

    CAS  PubMed  Google Scholar 

  34. Li T, Zhao Y, Matthews K, Gao J, Hao J, Wang S, Han J, Jia Y (2020) Antibacterial activity against Staphylococcus aureus of curcumin-loaded chitosan spray coupled with photodynamic treatment. LWT 134:110073

    CAS  Google Scholar 

  35. Liang H, Mirinejad MS, Asefnejad A, Baharifar H, Li X, Saber-Samandari S, Toghraie D, Khandan A (2022) Fabrication of tragacanthin gum-carboxymethyl chitosan bio-nanocomposite wound dressing with silver-titanium nanoparticles using freeze-drying method. Mater Chem Phys 279:125770

    CAS  Google Scholar 

  36. Mbhele Z, Salemane M, Van Sittert C, Nedeljković J, Djoković V, Luyt A (2003) Fabrication and characterization of silver− polyvinyl alcohol nanocomposites. Chem Mater 15(26):5019–5024

    CAS  Google Scholar 

  37. Menazea A, Ismail A, Awwad NS, Ibrahium HA (2020) Physical characterization and antibacterial activity of PVA/Chitosan matrix doped by selenium nanoparticles prepared via one-pot laser ablation route. J Market Res 9(5):9598–9606

    CAS  Google Scholar 

  38. Mishra A, Kaushik NK, Sardar M, Sahal D (2013) Evaluation of antiplasmodial activity of green synthesized silver nanoparticles. Colloids Surf, B 111:713–718

    CAS  Google Scholar 

  39. Moeini A, Pedram P, Makvandi P, Malinconico M, d’Ayala GG (2020) Wound healing and antimicrobial effect of active secondary metabolites in chitosan-based wound dressings: a review. Carbohyd Polym 233:115839

    CAS  Google Scholar 

  40. Nambi KN, Majeed SA, Taju G, Sivasubbu S, Babu VS, Hameed AS (2017) Effects of nicotine on zebrafish: a comparative response between a newly established gill cell line and whole gills. Comp Biochem Physiol C: Toxicol Pharmacol 195:68–77

    Google Scholar 

  41. Ninave PB, Patil SD (2022) Pharmacological screening of Acalypha indica L.: possible role in the treatment of asthma. J Ethnopharmacol 290:115093

    CAS  PubMed  Google Scholar 

  42. Okamoto Y, Kawakami K, Miyatake K, Morimoto M, Shigemasa Y, Minami S (2002) Analgesic effects of chitin and chitosan. Carbohyd Polym 49(3):249–252

    CAS  Google Scholar 

  43. Okur NÜ, Hökenek N, Okur ME, Ayla Ş, Yoltaş A, Siafaka PI, Cevher E (2019) An alternative approach to wound healing field; new composite films from natural polymers for mupirocin dermal delivery. Saudi Pharmaceutical J 27(5):738–752

    Google Scholar 

  44. Oladoja NA, Adelagun ROA, Ahmad AL, Unuabonah EI, Bello HA (2014) Preparation of magnetic, macro-reticulated cross-linked chitosan for tetracycline removal from aquatic systems. Colloids Surf, B 117:51–59

    CAS  Google Scholar 

  45. Parani M, Lokhande G, Singh A, Gaharwar AK (2016) Engineered nanomaterials for infection control and healing acute and chronic wounds. ACS Appl Mater Interfaces 8(16):10049–10069

    CAS  PubMed  Google Scholar 

  46. Paul A, Eun C-J, Song JM (2014) Cytotoxicity mechanism of non-viral carriers polyethylenimine and poly-l-lysine using real time high-content cellular assay. Polymer 55(20):5178–5188

    CAS  Google Scholar 

  47. Rajamohan R, Raorane CJ, Kim S-C, Lee YR (2022) One pot synthesis of copper oxide nanoparticles for efficient antibacterial activity. Materials 16(1):217

    PubMed  PubMed Central  Google Scholar 

  48. Rakshit S, Ghosh S, Chall S, Mati SS, Moulik SP, Bhattacharya SC (2013) Controlled synthesis of spin glass nickel oxide nanoparticles and evaluation of their potential antimicrobial activity: a cost effective and eco friendly approach. RSC Adv 3(42):19348–19356

    CAS  Google Scholar 

  49. Ramyadevi J, Jeyasubramanian K, Marikani A, Rajakumar G, Rahuman AA (2012) Synthesis and antimicrobial activity of copper nanoparticles. Mater Lett 71:114–116

    CAS  Google Scholar 

  50. Reddy JS, Rao PR, Reddy MS (2002) Wound healing effects of Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in rats. J Ethnopharmacol 79(2):249–251

    PubMed  Google Scholar 

  51. Rezagholizade-shirvan A, Najafi MF, Behmadi H, Masrournia M (2022) Preparation of nano-composites based on curcumin/chitosan-PVA-alginate to improve stability, antioxidant, antibacterial and anticancer activity of curcumin. Inorg Chem Commun 145:110022

    CAS  Google Scholar 

  52. Sakthiguru N, Manimohan M, Jaganathan G, Manivannan K, Sithique MA (2021) Biologically active Chitosan/ZnO/Acalypha indica leaf extract biocomposite: an investigation of antibacterial, cell proliferation and cell migration aptitude for wound healing application. Sustain Chem Pharm 19:100357

    CAS  Google Scholar 

  53. Schmedlen RH, Masters KS, West JL (2002) Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering. Biomaterials 23(22):4325–4332

    CAS  PubMed  Google Scholar 

  54. Sinha T, Bandyopadhaya A (2012) Ethno-pharmacological importance and valuable phytochemicals of Acalypha indica (L.) a review. Int J Res Pharm Sci 3:360–368

    Google Scholar 

  55. Slima SB, Trabelsi I, Ktari N, Bardaa S, Elkaroui K, Bouaziz M, Abdeslam A, Salah RB (2019) Novel Sorghum bicolor (L.) seed polysaccharide structure, hemolytic and antioxidant activities, and laser burn wound healing effect. Int J Biol Macromol 132:87–96

    PubMed  Google Scholar 

  56. Sophia A, Faiyazuddin M, Alam P, Hussain MT, Shakeel F (2022) GC–MS characterization and evaluation of antimicrobial, anticancer and wound healing efficiency of combined ethanolic extract of Tridax procumbens and Acalypha indica. J Mol Struct 1250:131678

    CAS  Google Scholar 

  57. Subramanian H, Santhaseelan H, Dinakaran VT, Devendiran V, Rathinam AJ, Mahalingam A, Ramachandran SK, Muthukumarasamy A, Muthukumar K, Mathimani T (2023) Hydrothermal synthesis of spindle structure copper ferrite-graphene oxide nanocomposites for enhanced photocatalytic dye degradation and in-vitro antibacterial activity. Environ Res 231:116095

    CAS  PubMed  Google Scholar 

  58. Sun L, Han J, Liu Z, Wei S, Su X, Zhang G (2019) The facile fabrication of wound compatible anti-microbial nanoparticles encapsulated Collagenous Chitosan matrices for effective inhibition of poly-microbial infections and wound repairing in burn injury care: exhaustive in vivo evaluations. J Photochem Photobiol, B 197:111539

    CAS  PubMed  Google Scholar 

  59. Tiwari M, Narayanan K, Thakar MB, Jagani HV, Venkata Rao J (2014) Biosynthesis and wound healing activity of copper nanoparticles. IET Nanobiotechnol 8(4):230–237

    PubMed  Google Scholar 

  60. Tong J, Hou X, Cui D, Chen W, Yao H, Xiong B, Cai L, Zhang H, Jiang L (2022) A berberine hydrochloride-carboxymethyl chitosan hydrogel protects against Staphylococcus aureus infection in a rat mastitis model. Carbohyd Polym 278:118910

    CAS  Google Scholar 

  61. Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG Jr (2015) Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 28(3):603–661

    CAS  PubMed  PubMed Central  Google Scholar 

  62. Topal Canbaz G, Keskin ZS, Yokuş A, Aslan R (2023) Biofabrication of copper oxide nanoparticles using Solanum tuberosum L. var. Vitelotte: characterization, antioxidant and antimicrobial activity. Chem Papers: 1–8

  63. Valenzuela-Rojo RD, López-Cervantes J, Sánchez-Machado DI, Escárcega-Galaz AA, del Rosario Martínez-Macias M (2020) Antibacterial, mechanical and physical properties of collagen-chitosan sponges from aquatic source. Sustain Chem Pharm 15:100218

    Google Scholar 

  64. Varaprasad K, Jayaramudu T, Kanikireddy V, Toro C, Sadiku ER (2020) Alginate-based composite materials for wound dressing application: a mini review. Carbohyd Polym 236:116025

    CAS  Google Scholar 

  65. Venkataprasanna K, Prakash J, Mathapati SS, Bharath G, Banat F, Venkatasubbu GD (2021) Development of chitosan/poly (vinyl alcohol)/graphene oxide loaded with vanadium doped titanium dioxide patch for visible light driven antibacterial activity and accelerated wound healing application. Int J Biol Macromol 193:1430–1448

    CAS  PubMed  Google Scholar 

  66. Venkataprasanna K, Prakash J, Vignesh S, Bharath G, Venkatesan M, Banat F, Sahabudeen S, Ramachandran S, Venkatasubbu GD (2020) Fabrication of Chitosan/PVA/GO/CuO patch for potential wound healing application. Int J Biol Macromol 143:744–762

    CAS  PubMed  Google Scholar 

  67. Venkatasubbu GD, Baskar R, Anusuya T, Seshan CA, Chelliah R (2016) Toxicity mechanism of titanium dioxide and zinc oxide nanoparticles against food pathogens. Colloids Surf, B 148:600–606

    CAS  Google Scholar 

  68. Vimal S, Taju G, Nambi KN, Majeed SA, Babu VS, Ravi M, Hameed AS (2012) Synthesis and characterization of CS/TPP nanoparticles for oral delivery of gene in fish. Aquaculture 358:14–22

    Google Scholar 

  69. Wang H, Zhang Y, Xue W, Zhang X, Jia X, Xue T, Guo R, Niu B, Yan H (2020) Preparation, characterization and antibacterial activity of a novel soluble polymer derived from xanthone and O-carboxymethyl-N, N, N-trimethyl chitosan. Int J Biol Macromol 164:836–844

    CAS  PubMed  Google Scholar 

  70. Wang Y, Shi D (2022) In vitro and in vivo evaluations of nanofibrous nanocomposite based on carboxymethyl cellulose/polycaprolactone/cobalt-doped hydroxyapatite as the wound dressing materials. Arab J Chem 15(11):104270

    CAS  Google Scholar 

  71. Wang Z, Ou X, Guan L, Li X, Liu A, Li L, Zvyagin AV, Qu W, Yang B, Lin Q (2023) Pomegranate-inspired multifunctional nanocomposite wound dressing for intelligent self-monitoring and promoting diabetic wound healing. Biosens Bioelectron 235:115386

    CAS  PubMed  Google Scholar 

  72. Xu C-X, Chen R-Y, Zheng X, Chen X, Chen Z (2008) Preparation of PVA-GA-CS/PVA-Fe-SA bipolar membrane and its application in electro-generation of 2, 2-dimethyl-3-hydroxypropionic acid. J Membr Sci 307(2):218–224

    CAS  Google Scholar 

  73. Yang X, Lan W, Xie J (2022) Antimicrobial and anti-biofilm activities of chlorogenic acid grafted chitosan against Staphylococcus aureus. Microb Pathog 173:105748

    CAS  PubMed  Google Scholar 

  74. Zahidin NS, Saidin S, Zulkifli RM, Muhamad II, Ya’akob H, Nur H (2017) A review of Acalypha indica L. (Euphorbiaceae) as traditional medicinal plant and its therapeutic potential. J Ethnopharmacol 207:146–173

    PubMed  Google Scholar 

  75. Zhang Q, Zhang M, Wang T, Chen X, Li Q, Zhao X (2022) Preparation of aloe polysaccharide/honey/PVA composite hydrogel: antibacterial activity and promoting wound healing. Int J Biol Macromol 211:249–258

    CAS  PubMed  Google Scholar 

  76. Zoe LH, David SR, Rajabalaya R (2023) Chitosan nanoparticle toxicity: a comprehensive literature review of in vivo and in vitro assessments for medical applications. Toxicol Rep

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Acknowledgements

This study is supported by Saveetha Medical College and Hospital. Authors are thankful to the institution for providing the infrastructure and support to carry out the research work.

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Authors and Affiliations

  1. Bioconversion and Tissue Engineering Laboratory, Department of Community Medicine, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai, 602105, India

    Aswathy Priyamvadan & Vinay Kumar

  2. Department of Chemistry, Sathyabama Institute of Science and Technology (Deemed To Be University), Chennai, Tamilnadu, India

    S. Thirugnanam

  3. Aquatic Animal Health Laboratory, C. Abdul Hakeem College, Melvishram, Ranipet District, Tamilnadu, India

    S. Abdul Majeed & A. S. Sahul Hameed

  4. Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai, 602105, India

    S. Vimal

  5. Department of Chemistry, Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Avadi, Chennai, 600062, India

    Sivarama Krishna Lakkaboyana

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  1. Aswathy Priyamvadan
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Priyamvadan, A., Thirugnanam, S., Majeed, S.A. et al. Chitosan PVA and Acalypha indica-based nanoformulation for antimicrobial activity against Staphylococcus aureus. Polym. Bull. 81, 8141–8158 (2024). https://doi.org/10.1007/s00289-023-05094-9

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