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Detection of naringin by fluorescent polarization molecularly imprinted polymer

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Abstract

A fluorescent magnetic surface molecular imprinting method was used to detect naringin by fluorescence polarization technology. By using SiO2-coated magnetic particles as substrate and methacrylic acid and acrylamide as monomers, a surface molecular imprinting polymer with both fluorescence and magnetic characteristics was prepared and loaded with fluorescein isothiocyanate. The binding ability of the prepared polymer was tested by fluorescence polarization and ultraviolet (UV) spectrophotometry. Compared with UV detection method, the fluorescence polarization method was more sensitive, and its limit of detection was 0.1 mg/L. The recovery of the fluorescence polarization method was higher than 81.3%. It was shown that the fluorescent magnetic surface molecular imprinting technique could be a new method to detect naringin quickly and efficiently in food.

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Abbreviations

TEM:

Transmission electron microscopy

UV:

Ultraviolet spectroscopy

HPLC:

High-performance liquid chromatography

MIPs:

Molecularly imprinted polymers

EGDE:

Ethylene glycol diglycidyl ether

TEOS:

Tetraethyl orthosilicate

FITC:

Fluorescein isothiocyanate

NG:

Naringin

TTS:

Triethoxyvinylsilane

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Acknowledgements

The authors are grateful to all the study participants.

Funding

This research was funded by Natural Science Foundation Research Project of Shaanxi Province of China, grant number 2018JQ2075.

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All authors have read and agree to the published version of the manuscript. Conceptualization, CZ; methodology, YR; formal analysis, GL; writing—original draft preparation, CZ; writing—review and editing, YR.

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Correspondence to Chen Zhao.

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Zhao, C., Ren, Y. & Li, G. Detection of naringin by fluorescent polarization molecularly imprinted polymer. Polym. Bull. 80, 1411–1424 (2023). https://doi.org/10.1007/s00289-022-04115-3

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  • DOI: https://doi.org/10.1007/s00289-022-04115-3

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