Skip to main content
SpringerLink
Log in

Transglutaminase effect on the gelatin-films properties

  • Original Paper
  • Published:
Polymer Bulletin Aims and scope Submit manuscript

Abstract

The development of biodegradable materials, especially those from renewable sources, is important to reduce the impact of plastic waste on the environment. On the other hand, protein-based films have high solubility in water, in addition to limited mechanical properties, which makes their application in high humidity environments a challenge. The enzyme transglutaminase (TGase) can reduce the interaction of gelatin films with water, improving these properties, while improving the mechanical properties. Therefore, this study aimed to determine the best condition for using the TGase enzyme, through variations in the gelatin and enzyme mass, in addition to the time that the enzyme acts on the gelatin, evaluating the properties tensile strength, elongation, and solubility in water. In the second set of experiments, gelatin amount was kept fixed at 4%, and other proportions of an enzyme (1% and 5% w/w gelatin) were studied, evaluating beyond these properties the degradation in simulated soil through thermogravimetry analysis and Fourier transform infrared spectrometer (FTIR). It was concluded that the higher concentration of TGase (5%) promoted a greater reduction in the solubility of the films, making the films more resistant to biodegradation, facilitating their application due to the increase in their useful life. At the end of the degradation test, it was noticed that the films were degraded, presenting the potential for substitution of polymers of fossil origin, which could be an alternative to the problem of polymeric residues in the environment.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Rosseto M, Krein DD, Balbé NP, Dettmer A (2019) Starch–gelatin film as an alternative to the use of plastics in agriculture: a review. J Sci Food Agric 99:6671–6679. https://doi.org/10.1002/jsfa.9944

    Article  CAS  PubMed  Google Scholar 

  2. Plastics Europe. Plastics – the Facts 2019 (2020). Available at: https://www.plasticseurope.org/application/files/9715/7129/9584/FINAL_web_version_Plastics_the_facts2019_14102019.pdf. Accessed 13 Oct 2020

  3. Júnior LM, Vieira RP, Jamróz E, Anjos CAR (2021) Furcellaran: an innovative biopolymer in the production of films and coatings Carbohydr. Polym 252:117221. https://doi.org/10.1016/j.carbpol.2020.117221

    Article  CAS  Google Scholar 

  4. Sharma R, Jafari SM, Sharma S (2020) Antimicrobial bio-nanocomposites and their potential applications in food packaging. Food Control 112:107086. https://doi.org/10.1016/j.foodcont.2020.107086

    Article  CAS  Google Scholar 

  5. Rigueto CVT, Nazari MT, Rosseto M, Massuda LA, Alessandretti I, Piccin JS, Dettmer A (2021) Emerging contaminants adsorption by beads from chromium (III) tanned leather waste recovered gelatin. J Mol Liq 330:115638. https://doi.org/10.1016/j.molliq.2021.115638

    Article  CAS  Google Scholar 

  6. Rigueto CVT, Nazari MT, Massuda LÁ, Ostwald BEP, Piccin JS, Dettmer A (2021) Production and environmental applications of gelatin-based composite adsorbents for contaminants removal: a review Environ. Chem Lett. https://doi.org/10.1007/s10311-021-01184-0

    Article  Google Scholar 

  7. Weng S, López A, Sáez-Orviz S, Marcet I, García P, Rendueles M, Díaz M (2020) Effectiveness of bacteriophages incorporated in gelatin films against Staphylococcus aureus. Food Control 121:107666. https://doi.org/10.1016/j.foodcont.2020.107666

    Article  CAS  Google Scholar 

  8. Hanani NZ, Ross HY, Kerry PJ (2014) Use and application of gelatin as potential biodegradable packaging materials for food packaging Int. J Biol Macromol 71:94–102. https://doi.org/10.1016/j.ijbiomac.2014.04.027

    Article  CAS  Google Scholar 

  9. Scopel BS, Restelatto D, Baldasso C, Dettmer A, Santana RMC (2018) Steam Explosion in alkaline medium for gelatin extraction from chromium-tanned leather wastes: time reduction and process optimization: time reduction and process optimization Environ. Technol 41:1–10. https://doi.org/10.1080/09593330.2018.1551430

    Article  CAS  Google Scholar 

  10. Jiang S, Zou L, Hou Y, Qian F, Tuo Y, Wu X, Mu G (2020) The influence of the addition of transglutaminase at different phase on the film and film forming characteristics of whey protein concentrate-carboxymethyl chitosan composite films Food Packag. Shelf Life 25:100546. https://doi.org/10.1016/j.fpsl.2020.100546

    Article  Google Scholar 

  11. Cheng S, Wang W, Li Y, Gao G, Zhang K, Zhou J, Wu Z (2019) Cross-linking and film- forming properties of transglutaminase-modified collagen fibers tailored by denaturation temperature. Food Chem 271:527–535. https://doi.org/10.1016/j.foodchem.2018.07.223

    Article  CAS  PubMed  Google Scholar 

  12. Vera A, Tapia C, Abugoch L (2020) Effect of high-intensity ultrasound treatment in combination with transglutaminase and nanoparticles on structural, mechanical, and physicochemical properties of quinoa proteins/chitosan edible films. Int J Biol Macromol 144:536–543. https://doi.org/10.1016/j.ijbiomac.2019.12.120

    Article  CAS  PubMed  Google Scholar 

  13. ASTM D882. Standart test method for tensile properties of thin plastic sheeting (2012) ASTM International. Available at: https://www.astm.org/Standards/D882. Accessed 13 Oct 2020

  14. ASTM E96. Standard Test Methods for Water Vapor Transmission of Materials (2000) ASTM International. Available at: https://www.astm.org/Standards/E96. Accessed 13 Oct 2020

  15. Cuq B, Gontard N, Cuq JL, Guilbert S (1997) Selected functional properties of fish myofribilar protein-based films affected by hidrophilic plasticizers. J Agric Food Chemis 45:622–626. https://doi.org/10.1021/jf960352i

    Article  CAS  Google Scholar 

  16. ASTM G160. Standard Practice for Evaluating Microbial Susceptibility of Nonmetallic Materials by Laboratory Soil Burial (2003) ASTM International. Available at: https://www.astm.org/Standards/G160.htm. Accessed 13 Oct 2020

  17. Chambi H, Grosso C (2006) Edible films produced with gelatin and casein cross-linked with TGase. Food Res Int 39:458–466. https://doi.org/10.1016/j.foodres.2005.09.009

    Article  CAS  Google Scholar 

  18. Ceresino EB, Kuktaite R, Hedenqvist MS, Sato HH, Johansson E (2020) Processing conditions and TGase sources to “drive” the wheat gluten dough quality Innovative Food Sci. Emerging Technol 65:102439. https://doi.org/10.1016/j.ifset.2020.102439

    Article  CAS  Google Scholar 

  19. Baggio E, Scopel BS, Krein DDC, Rigueto CVT, Baldasso C, Dettmer A (2021) Transglutaminase crosslinked gelatin films extracted from tanned leather waste. J Am Leather Chem As 116:1–36

    Google Scholar 

  20. Wang H, Wei D, Zheng A, Xiao H (2015) Soil burial biodegradation of antimicrobial biodegradable PBAT films. Polym Degrad Stab 116:14–22. https://doi.org/10.1016/j.polymdegradstab.2015.03.007

    Article  CAS  Google Scholar 

  21. Al-Hassan AA, Norziah HN (2017) Effect of transglutaminase induced crosslinking on the properties of starch/ gelatin films Food Packag. Shelf Life 13:15–19. https://doi.org/10.1016/j.fpsl.2017.04.006

    Article  Google Scholar 

  22. Rosseto M, Rigueto CVT, Krein DDC, Massuda LA, Ostwald BEP, Colla LM, Dettmer A (2020) Accelerated aging of starch-gelatin films with enzymatic treatment. J Polym Environ 29:1–13. https://doi.org/10.1007/s10924-020-01938-x

    Article  CAS  Google Scholar 

  23. Hwang E, Kim K, Lee CG, Kwon TH, Lee SH, Min SK, Kim BS (2020) Tailorable degradation of pH-responsive all-polyether micelles: unveiling the role of monomer structure and hydrophilic-hydrophobic balance. Macromol 52:5884–5893. https://doi.org/10.1021/acs.macromol.9b00823

    Article  CAS  Google Scholar 

  24. Prudnikova SV, Evgrafova SY, Volova TG (2021) Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics. Chemosphere. 263:128180. https://doi.org/10.1016/j.chemosphere.2020.128180

    Article  CAS  PubMed  Google Scholar 

  25. Briassoulis D (2006) Mechanical behaviour of biodegradable agricultural films under real field conditions. Polym. Degrad. Stabil. 91:1256–1272. https://doi.org/10.1016/j.polymdegradstab.2005.09.016

    Article  CAS  Google Scholar 

  26. Scopel BS, Pretto GL, Correa PJI, Baldasso C, Dettmer A, Santana CRM (2020) Starch-leather waste gelatin films cross-linked with glutaraldehyde. J Polym Environ 28:1974–1984. https://doi.org/10.1007/s10924-020-01736-5

    Article  CAS  Google Scholar 

  27. Rigueto CVT, Rosseto M, Krein DDC, Ostwald BEP, Massuda L, Zanella BB, Dettmer A (2020) Alternative uses for tannery wastes: a review of environmental, sustainability, and science. J Leather Sci Eng 2:1–20. https://doi.org/10.1186/s42825-020-00034-z

    Article  Google Scholar 

  28. Wang W, Wang K, Xiao J, Liu Y, Zhao Y, Liu A (2017) Performance of high amylose starch-composited gelatin films influenced by gelatinization and concentration Int. J Biol Macromol 94:258–265. https://doi.org/10.1016/j.ijbiomac.2016.10.014

    Article  CAS  Google Scholar 

  29. Araújo JM (1999) Food Chemistry - Theory and Practice [in portuguese]. 2nd ed. Federal University of Viçosa, Viçosa-MG

  30. Alshehrei F (2017) Biodegradation of synthetic and natural plastic by microorganisms. J Appl Environ Microbiol 5:8–19. https://doi.org/10.12691/jaem-5-1-2

    Article  CAS  Google Scholar 

  31. Nagarajan M, Benjakul S, Prodpran T, Songtipya P, Kishimura H (2012) Characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocoll. 29:389–397. https://doi.org/10.1016/j.foodhyd.2012.04.001

    Article  CAS  Google Scholar 

  32. Wu X, Liu A, Wang W (2018) Improved mechanical properties and thermal-stability of collagen fiber-based film by crosslinking with casein, keratin or SPI: effect of crosslinking process and concentrations of proteins Int. J Biol Macromol 109:1319–1328. https://doi.org/10.1016/j.ijbiomac.2017.11.144

    Article  CAS  Google Scholar 

  33. Kaewruang P, Benjakul S, Prodpran T, Nalinanon S (2013) Physicochemical and functional properties of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as affected by extraction conditions. Food Biosc. 2:1–9. https://doi.org/10.1016/j.fbio.2013.03.002

    Article  CAS  Google Scholar 

  34. Jamróz E, Kopel P, Juszczak L, Kawecka A, Bytesnikova Z, Milosavljević V, Adam V (2018) Development and characterisation of furcellaran-gelatin films containing SeNPs and AgNPs that have antimicrobial activity. Food Hydrocoll 83:9–16. https://doi.org/10.1016/j.foodhyd.2018.04.028

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The University of Caxias do Sul (UCS). Sponsors had no involvement in this study other than providing necessary resources for its execution.

Funding

This study was realized thanks to the financial support of the Brazilian National Council for Technological and Scientific Development (CNPq), the Rio Grande do Sul State’s Research Support Foundation (FAPERGS).

Author information

Authors and Affiliations

  1. Postgraduate Program in Process and Technology Engineering (PGEPROTEC), University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, 95070-560, Brazil

    Elizete Baggio, Bianca Santinon Scopel & Camila Baldasso

  2. Faculty of Agronomy and Veterinary Medicine (FAMV), Post-Graduation Program in Food Science and Technology (PPGCTA), University of Passo Fundo, Passo Fundo, Rio Grande do Sul, 99052-900, Brazil

    Marieli Rosseto, Cesar Vinicius Toniciolli Rigueto & Aline Dettmer

Authors
  1. Elizete Baggio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bianca Santinon Scopel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marieli Rosseto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cesar Vinicius Toniciolli Rigueto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aline Dettmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Camila Baldasso
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Elizete Baggio contributed to investigation and validation; Bianca Santinon Scopel contributed to investigation and validation; Marieli Rosseto contributed to writing—original draft preparation, and writing—reviewing and editing; Cesar Vinicius Toniciolli contributed to writing—original draft preparation, and writing—reviewing and editing; Aline Dettmer contributed to project administration, data curation, and methodology; Camila Baldasso helped in visualization and supervision.

Corresponding author

Correspondence to Aline Dettmer.

Ethics declarations

Conflicts of interest

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 457 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Baggio, E., Scopel, B.S., Rosseto, M. et al. Transglutaminase effect on the gelatin-films properties. Polym. Bull. 79, 7347–7361 (2022). https://doi.org/10.1007/s00289-021-03858-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00289-021-03858-9

Keywords

Search

Navigation