Abstract
In this study, 5-fluorouracil (5-FU) entrapped polycaprolactone nanoparticles (5-FU- PCNPs) have been prepared using double emulsion method. The different factors were examined for assembly to arrive at the best effective formulation of 5-FU-PCNPs formulation for 5-FU–PCNPs, as polymer concentration, stabilizer concentration. The encapsulation efficiency of PCNPs was in the range of 18.8–45.4%. The prepared nanoparticles showed the spherical shape having an average size of 183–675.5 nm, whereas TEM exhibited the prepared nanoparticles have a spherical shape. FTIR, XRPD, confirmed successful insertion of drug in prepared PCNPs. In vitro release of 5-FU from selected formulations showed sustained release from the nanoparticles where slower release was observed when lower PVA concentration was used. Anticancer activity was examined against cell culture for HCT-116 (human colorectal carcinoma), MCF-7(human breast adenocarcinoma), HepG2 (human hepatocellular carcinoma) and A549 (human lung carcinoma) for six formulations 5-FU–PCNPs nanoparticles. The in vitro cytotoxic activity of the prepared formulations was tested showing that these formulations appeared as promising active anticancer formulations.
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MS was involved in conceptualization, methodology, investigation, resources, data curation, writing—reviewing and editing. HMA contributed to methodology, visualization, conducted the biological assays and provided the experimental procedures and results of biological part. MMHA was involved in conceptualization, methodology, writing—reviewing and editing, visualization, funding acquisition.
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Samy, M., Abdallah, H.M., Awad, H.M. et al. In vitro release and cytotoxicity activity of 5-fluorouracil entrapped polycaprolactone nanoparticles. Polym. Bull. 79, 6645–6671 (2022). https://doi.org/10.1007/s00289-021-03804-9
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DOI: https://doi.org/10.1007/s00289-021-03804-9