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Morphological and spectroscopical characterization of hyperbranched polyamidoamine–zwitterionic chitosan-encapsulated 5-FU anticancer drug

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Abstract

Hyperbranched polyamidoamines (PAMAM) have been widely investigated for therapeutic applications but their cytotoxicity caused by their cationic charges limited their spreading use in biomedical applications. Zwitterionic chitosan (ZWCs) is a biocompatible, pH sensitive and nontoxic polymer which can be used to reduce cytotoxicity by covering the PAMAM surface. The complex formed from synthesized PAMAM and ZWCs with different ratios was prepared at pH 7.4. Encapsulation of 5-Fluorouracil by these complexes has successfully been established for high efficiency drug delivery. The complex with ratio (3:1) ZWCs/PAMAM had average size ~ 310 nm and negatively charged at neutral pH confirming the ability of the ZWCs to cover PAMAM surface. The encapsulation efficiency ranged between 100 and 72.5% with loading capacity from 5 to 20% for different initial feed drug concentration 5–20 mg/ml. The release profile of ZWC–PAMAM complex showed sustained drug release up to period of 38 h.

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Acknowledgements

The authors would like to acknowledge Dr A.P. Filippov, S.V. Valueva and A.A. Kutin; Institute of Macromolecular Compounds, Russian Academy of Sciences for their potential efforts in electron microscopy, Uv-spectroscopy and Dynamic light scattering measurements in this work. Also, the authors would like to acknowledge Science, Technology and Innovation Funding Authority STIFA for its grant No. 26599.

Funding

This research is supported by the grants of Sciences, Technology and innovation Funding Authority (STIFA) No.26599 and the Russian Foundation for Basic Research (RFBR) No.17–53-61026 Egypt a.

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Authors and Affiliations

  1. Polymers and Pigments Department, National Research Centre, 33 Elbehouth St. Dokki, Giza, 12622, Egypt

    Eman H. Ahmed, Ahmed E. Abdelhamid & Magdy M. H. Ayoub

  2. Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoj pr. V.O., 31, St.-Petersburg, Russia, 199004

    M. E. Vylegzhanina, A. Ya. Volkov & T. E. Sukhanova

  3. S.V. Lebedev Institute of Synthetic Rubber, Gapsal’skaya str. 1, St.-Petersburg, Russia, 198035

    T. E. Sukhanova

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  1. Eman H. Ahmed
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Ahmed, E.H., Abdelhamid, A.E., Vylegzhanina, M.E. et al. Morphological and spectroscopical characterization of hyperbranched polyamidoamine–zwitterionic chitosan-encapsulated 5-FU anticancer drug. Polym. Bull. 79, 137–155 (2022). https://doi.org/10.1007/s00289-020-03495-8

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  • DOI: https://doi.org/10.1007/s00289-020-03495-8

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