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Enzymatic degradation of aqueous dextrans as affected by initial molecular weight and concentration

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Abstract

Dextrans with different initial molecular weights (IMW) ranging from 3.86 × 104 Da to 2.15 × 106 Da were enzymatically degraded by dextranase enzyme. The changes in weight-average molecular weight (Mw), polydispersity index (D value) and the percentage of 104–105 Da fragment of aqueous dextran were examined during 0–120 min enzymatic degradation. Results showed that the Mw of all dextran samples decreased with increasing enzymatic time. A significant molecular weight degradation occurred in dextran samples that had a higher IMW and at an early degradation stage. With increasing the degradation time, the percentage of the 104–105 Da in four dextran samples (Dex-270, Dex-1000, Dex-2000 and Dex-F) showed a remarkable growth at the beginning of the treatment (0–50 min). Experimental data indicated that all dextran samples (with different IMW and at different concentration) followed a second-order degradation kinetics. This study demonstrated a great potential for further production of low molecular weight dextrans with a more homologous molecular weight.

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Acknowledgements

The authors wish to acknowledge the Project of National Natural Science Foundation of China (Grand Nos. 21264003 and 21768001) for the support of the study.

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Author notes

  1. Hualei Chen and Yuanyuan Pu contributed equally to this study and they are co-first authors of the paper.

Authors and Affiliations

  1. College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, People’s Republic of China

    Hualei Chen, Yuanyuan Pu, Qingsong Zou, Dianzhi Hou & Shan Chen

  2. Center for Sugar Engineering and Technology Research, Guangxi University, Nanning, 530004, People’s Republic of China

    Hualei Chen, Yuanyuan Pu, Qingsong Zou, Dianzhi Hou & Shan Chen

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  1. Hualei Chen
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  2. Yuanyuan Pu
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Correspondence to Shan Chen.

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Chen, H., Pu, Y., Zou, Q. et al. Enzymatic degradation of aqueous dextrans as affected by initial molecular weight and concentration. Polym. Bull. 78, 4863–4876 (2021). https://doi.org/10.1007/s00289-020-03351-9

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