Abstract
Regeneration of damaged bone tissue by using degradable scaffold is the aim of bone tissue engineering. Herein, to enhance mechanical properties and osteointegration, we produced poly hydroxybutyrate-co-hexanoate (PHBHX) scaffolds containing hydroxyapatite and oxidized xanthan gum (OXG). Morphological and physicochemical properties of the scaffolds were determined. The effects of scaffolds on bone cells were investigated. When the results were evaluated, incorporation of OXG enhanced mechanical properties and swelling rate of the scaffolds. The scaffolds did not trigger of cytochrome-c release of Saos-2 cells and TNF-α expression of THP-1 cells. It was seen that incorporation of OXG to PHBHX scaffolds increased Saos-2 cell proliferation. As a result, the scaffolds were found to have clinical application potential.
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Barabaschi GDG, Manoharan V, Li Q, Bertassoni LE (2015) Engineering Pre-vascularized Scaffolds for bone regeneration. In: Bertassoni LE, Coelho PG (eds) Engineering mineralized and load bearing tissues. Springer, Cham, pp 79–94
Gómez S, Vlad MD, López J, Fernández E (2016) Design and properties of 3D scaffolds for bone tissue engineering. Acta Biomater 42:341–350. https://doi.org/10.1016/j.actbio.2016.06.032
Bandyopadhyay-Ghosh S (2008) Bone as a collagen-hydroxyapatite composite and its repair. Trends Biomater Artif Organ 22:116–124
Di Silvio L, Dalby MJ, Bonfield W (2002) Osteoblast behaviour on HA/PE composite surfaces with different HA volumes. Biomaterials 23:101–107
Wahab IF, Razak SIA (2016) Polysaccharides as composite biomaterials. In: Composites from Renewable and Sustainable Materials, Chap 4, pp 65–85
Ahmad N, Al-Subaie AM, Ahmad R et al (2019) Brain-targeted glycyrrhizic-acid-loaded surface decorated nanoparticles for treatment of cerebral ischaemia and its toxicity assessment. Artif Cells Nanomed Biotechnol 47:475–490
Bueno VB, Bentini R, Catalani LH, Petri DFS (2013) Synthesis and swelling behavior of xanthan-based hydrogels. Carbohydr Polym 92:1091–1099. https://doi.org/10.1016/j.carbpol.2012.10.062
Izawa H, Nishino S, Maeda H et al (2014) Mineralization of hydroxyapatite upon a unique xanthan gum hydrogel by an alternate soaking process. Carbohydr Polym 102:846–851
Bueno VB, Bentini R, Catalani LH et al (2014) Synthesis and characterization of xanthan–hydroxyapatite nanocomposites for cellular uptake. Mater Sci Eng C 37:195–203
Kumar A, Rao KM, Kwon SE et al (2017) Xanthan gum/bioactive silica glass hybrid scaffolds reinforced with cellulose nanocrystals: Morphological, mechanical and in vitro cytocompatibility study. Mater Lett 193:274–278
Biernacki M, Marzec M, Roick T et al (2017) Enhancement of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production. Microb Cell Fact 16(1):144
Biernacki M, Marzec M, Roick T et al (2017) Enhancement of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production. Microb Cell Fact 16:144
Chen G, Zhang G, Park S, Lee S (2001) Industrial scale production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate). Appl Microbiol Biotechnol 57:50–55
Wang Y, Bian Y-Z, Wu Q, Chen G-Q (2008) Evaluation of three-dimensional scaffolds prepared from poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits. Biomaterials 29:2858–2868
Wei X, Hu Y, Xie W et al (2009) Influence of poly (3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) on growth and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Biomed Mater Res Part A Off J Soc Biomater Jpn Soc Biomater Aust Soc Biomater Korean Soc Biomater 90:894–905
Zhao S, Zhu M, Zhang J et al (2014) Three dimensionally printed mesoporous bioactive glass and poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds for bone regeneration. J Mater Chem B 2:6106–6118
Freier T, Kunze C, Nischan C et al (2002) In vitro and in vivo degradation studies for development of a biodegradable patch based on poly (3-hydroxybutyrate). Biomaterials 23:2649–2657
Qu X-H, Wu Q, Zhang K-Y, Chen GQ (2006) In vivo studies of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) based polymers: biodegradation and tissue reactions. Biomaterials 27:3540–3548. https://doi.org/10.1016/j.biomaterials.2006.02.015
Guo J, Ge L, Li X et al (2014) Periodate oxidation of xanthan gum and its crosslinking effects on gelatin-based edible films. Food Hydrocoll 39:243–250
Pankongadisak P, Jaikaew N, Kiti K et al (2019) The potential use of gentamicin sulfate-loaded poly (l-lactic acid)-sericin hybrid scaffolds for bone tissue engineering. Polym Bull 76:2867–2885
Wang Y-W, Wu Q, Chen J, Chen G-Q (2005) Evaluation of three-dimensional scaffolds made of blends of hydroxyapatite and poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) for bone reconstruction. Biomaterials 26:899–904
Demirbilek M (2015) Effect of tributyl citrate on chitosan scaffold: chemical, physical properties, pro/anti inflammation cytokines. J Porous Mater 22:395–402
Paiva D, Gonçalves C, Vale I et al (2016) Oxidized Xanthan Gum and Chitosan as natural adhesives for cork. Polymers (Basel) 8(7):259–272. https://doi.org/10.3390/polym8070259
Boyan BD, Hummert TW, Dean DD, Schwartz Z (1996) Role of material surfaces in regulating bone and cartilage cell response. Biomaterials 17:137–146
Purohit SD, Bhaskar R, Singh H et al (2019) Development of a nanocomposite scaffold of gelatin–alginate–graphene oxide for bone tissue engineering. Int J Biol Macromol 133:592–602
Delattre C, Pierre G, Gardarin C et al (2015) Antioxidant activities of a polyglucuronic acid sodium salt obtained from TEMPO-mediated oxidation of xanthan. Carbohydr Polym 116:34–41
Hazirah MN, Isa MIN, Sarbon NM (2016) Effect of xanthan gum on the physical and mechanical properties of gelatin-carboxymethyl cellulose film blends. Food Packag Shelf Life 9:55–63
Li X, Feng Q (2005) Porous poly-L-lactic acid scaffold reinforced by chitin fibers. Polym Bull 54:47–55
Srinivasan S, Jayasree R, Chennazhi KP et al (2012) Biocompatible alginate/nano bioactive glass ceramic composite scaffolds for periodontal tissue regeneration. Carbohydr Polym 87:274–283
Jeon H, Lee H, Kim G (2014) A surface-modified poly(ɛ-caprolactone) scaffold comprising variable nanosized surface-roughness using a plasma treatment. Tissue Eng Part C Methods 20:951–963. https://doi.org/10.1089/ten.TEC.2013.0701
Maji K, Dasgupta S (2017) Effect of βtricalcium phosphate nanoparticles additions on the properties of gelatin–chitosan scaffolds. Bioceram Dev Appl 7:2–13
Dalby MJ, Di Silvio L, Harper EJ, Bonfield W (2002) Increasing hydroxyapatite incorporation into poly (methylmethacrylate) cement increases osteoblast adhesion and response. Biomaterials 23:569–576
Rentsch C, Rentsch B, Breier A et al (2010) Evaluation of the osteogenic potential and vascularization of 3D poly (3) hydroxybutyrate scaffolds subcutaneously implanted in nude rats. J Biomed Mater Res Part A Off J Soc Biomater Jpn Soc Biomater Aust Soc Biomater Korean Soc Biomater 92:185–195
Shi M, Zhai D, Zhao L et al (2014) Nanosized mesoporous bioactive glass/poly(lactic-co-glycolic acid) composite-coated CaSiO3 scaffolds with multifunctional properties for bone tissue engineering. Biomed Res Int 2014:323046. https://doi.org/10.1155/2014/323046
Ahmad N, Ahmad I, Umar S et al (2016) PNIPAM nanoparticles for targeted and enhanced nose-to-brain delivery of curcuminoids: UPLC/ESI-Q-ToF-MS/MS-based pharmacokinetics and pharmacodynamic evaluation in cerebral ischemia model. Drug Deliv 23:2095–2114
Kim JJ, Lee SB, Park JK, Yoo YD (2010) TNF-α-induced ROS production triggering apoptosis is directly linked to Romo1 and Bcl-X L. Cell Death Differ 17:1420–1434
McCarthy ET, Sharma R, Sharma M et al (1998) TNF-alpha increases albumin permeability of isolated rat glomeruli through the generation of superoxide. J Am Soc Nephrol 9:433–438
Brenner DA, Buck M, Feitelberg SP, Chojkier M (1990) Tumor necrosis factor-alpha inhibits albumin gene expression in a murine model of cachexia. J Clin Investig 85:248–255
Cardona MA, Simmons RL, Kaplan SS (1992) TNF and IL-1 generation by human monocytes in response to biomaterials. J Biomed Mater Res 26:851–859
Ding T, Sun J, Zhang P (2007) Immune evaluation of biomaterials in TNF-α and IL-1β at mRNA level. J Mater Sci Mater Med 18:2233–2236. https://doi.org/10.1007/s10856-007-3014-9
Donaldson AR, Tanase CE, Awuah D, et al (2018) Photocrosslinkable gelatin hydrogels modulate the production of the major pro-inflammatory cytokine, TNF-α, by human mononuclear cells. Front Bioeng Biotechnol 6
Kannan K, Jain SK (2000) Oxidative stress and apoptosis. Pathophysiology 7:153–163
Hüttemann M, Pecina P, Rainbolt M et al (2011) The multiple functions of cytochrome c and their regulation in life and death decisions of the mammalian cell: From respiration to apoptosis. Mitochondrion 11:369–381
Méndez J, Morales Cruz M, Delgado Y et al (2014) Delivery of chemically glycosylated cytochrome c immobilized in mesoporous silica nanoparticles induces apoptosis in HeLa cancer cells. Mol Pharm 11:102–111. https://doi.org/10.1021/mp400400j
Moseley R, Leaver M, Walker M et al (2002) Comparison of the antioxidant properties of HYAFF®-11p75, AQUACEL® and hyaluronan towards reactive oxygen species in vitro. Biomaterials 23:2255–2264
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This study was conducted at Hacettepe University Advanced Technologies Application and Research Center (HUNITEK) and not supported by any institution. There is no conflict between the authors.
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Arslan, A.K., Alkan, F. PHBHX–HA–OXG bone graft: in-vitro characterization. Polym. Bull. 78, 1835–1849 (2021). https://doi.org/10.1007/s00289-020-03187-3
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DOI: https://doi.org/10.1007/s00289-020-03187-3