Abstract
In this paper, chitin nano-whiskers (CNWs) were prepared through acid hydrolysis. The surface deacetylation of the prepared CNW in alkali conditions was carried out. The morphologies, crystallinity, degree of deacetylation and antibacterial property of the nano-whiskers were studied by transmission electron microscopy (TEM), Fourier transform infrared spectra, X-ray diffraction, antibacterial testing and so on. The results confirmed that the amino groups were successfully introduced onto the surface of the CNW, so that the nano-rod-like morphology of the whisker was still maintained. The degree of deacetylation of CNW increased from 4.4% up to a maximum of 55.2% with the increase in NaOH concentration and reaction temperature. Furthermore, deacetylation kinetics of CNW was also investigated. It was found that the deacetylation process followed the pseudo-first-order kinetics. The apparent rate constant of the reaction increased with the increase in NaOH concentration and reaction temperature. The apparent activation energy of the reaction was estimated as 27.71 kJ/mol. The deacetylated CNW exhibited much improved antibacterial property, and the percentage bacterial reduction of the sample reached 85%.
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Zeng JB, He YS, Li SL, Wang YZ (2011) Chitin whiskers: an overview. Biomacromol 13:1–11
Gopalan NK, Dufresne A (2003) Crab shell chitin whisker reinforced natural rubber nanocomposites. 2. Mechanical behavior. Biomacromol 4:666–674
Qin Y, Zhang SL, Yu J, Yang J, Xiong L, Sun QJ (2016) Effects of chitin nano-whiskers on the antibacterial and physicochemical properties of maize starch films. Carbohydr Polym 147:372–378
Junkasem J, Rujiravanit R, Grady BP, Supaphol P (2010) X-ray diffraction and dynamic mechanical analyses of α-chitin whisker-reinforced poly(vinyl alcohol) nanocomposite nanofibers. Polym Int 59:85–91
Junkasem J, Rujiravanit R, Supaphol P (2006) Fabrication of α-chitin whisker-reinforced poly (vinyl alcohol) nanocomposite nanofibres by electrospinning. Nanotechnology 17:4519–4528
Sriupayo J, Supaphol P, Blackwell J, Rujiravanit R (2005) Preparation and characterization of α-chitin whisker-reinforced poly (vinyl alcohol) nanocomposite films with or without heat treatment. Polymer 46:5637–5644
Wang QQ, Chen SY, Chen DJ (2017) Preparation and characterization of chitosan based injectable hydrogels enhanced by chitin nano-whiskers. J Mech Behav Biomed 65:466–477
Angulakhsmi N, Thomas S, Nair JR, Bongiovanni R, Gerbaldi C, Stephan AM (2013) Cycling profile of innovative nanochitin-incorporated poly (ethylene oxide) based electrolytes for lithium batteries. J Power Sources 228:294–299
Bogdanova OI, Polyakov DK, Streltsov DR, Kulebyakina AI, Orekhov AS, Vasiliev AL, Blackwell J, Chvalun SN (2017) Fabrication and mechanical properties of composite based on β-chitin and polyacrylic acid. Carbohydr Polym 157:1496–1502
Lin N, Huang J, Dufresne A (2012) Preparation, properties and applications of polysaccharide nanocrystals in advanced functional nanomaterials: a review. Nanoscale 4:3274–3294
Feng LD, Zhou ZY, Dufresne A, Huang J, Wei M, An LJ (2009) Structure and properties of new thermoforming bionanocomposites based on chitin whisker-graft-polycaprolactone. J Appl Polym Sci 112:2830–2837
Li CR, Liu H, Luo BH, Wen W, He LM, Liu MX, Zhou CR (2016) Nanocomposites of poly (l-lactide) and surface-modified chitin whiskers with improved mechanical properties and cytocompatibility. Eur Polym J 81:266–283
Wang BJ, Li J, Zhang JQ, Li HY, Chen P, Gu Q, Wang ZB (2013) Thermo-mechanical properties of the composite made of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) and acetylated chitin nanocrystals. Carbohydr Polym 95:100–106
Dutta A, Egusa M, Kaminaka H, Izawa H, Morimoto M, Saimoto H, Ifuku S (2015) Facile preparation of surface N-halamine chitin nanofiber to endow antibacterial and antifungal activities. Carbohydr Polym 115:342–347
Lertwattanaseri T, Ichikawa N, Mizoguchi T, Tanaka Y, Chirachanchai S (2009) Microwave technique for efficient deacetylation of chitin nanowhiskers to a chitosan nanoscaffold. Carbohydr Res 344:331–335
Phongying S, Aiba S, Chirachanchai S (2007) Direct chitosan nanoscaffold formation via chitin whiskers. Polymer 48:393–400
Pereira AG, Muniz EC, Hsieh YL (2014) Chitosan-sheath and chitin-core nanowhiskers. Carbohydr Polym 107:158–166
Segal L, Creely JJ, Martin AE Jr, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the x-ray diffractometer. Text Res J 29:786–794
Ye W, Leung MF, Xin J, Kwong TL, Lee DKL, Li P (2005) Novel core-shell particles with poly (n-butyl acrylate) cores and chitosan shells as an antibacterial coating for textiles. Polymer 46:10538–10543
Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31:603–632
Baxter A, Dillon M, Taylor KD, Roberts GA (1992) Improved method for i.r. determination of the degree of N-acetylation of chitosan. Int J Biol Macromol 14:166–169
Li CR, Liu H, Luo BH, Wen W, He LM, Liu MX, Zhou CR (2016) Nanocomposites of poly(l -lactide) and surface-modified chitin whiskers with improved mechanical properties and cytocompatibility. Eur Polym J 81:266–283
Sannan T, Kurita K, Iwakura Y (1977) Studies on Chitin. V. Kinetics of deacetylation reaction. Polym J 9:649–651
Rabea EI, Badawy MET, Stevens CV, Smagghe G, Steurbaut W (2003) Chitosan as antimicrobial agent: applications and mode of action. Biomacromol 4:1457–1465
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Chen, S., Chen, D. Surface deacetylation of chitin nano-whiskers. Polym. Bull. 77, 5345–5355 (2020). https://doi.org/10.1007/s00289-019-03018-0
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DOI: https://doi.org/10.1007/s00289-019-03018-0