Abstract
Electrospun poly (Ɛ-caprolactone) (PCL) has been widely utilized as a biomedical scaffold material to regenerate damaged tissues. However, an electrospun scaffold made from PCL can have some drawbacks such as lack of surface bioactivity, low cell adhesion, as well as osteoinduction, which often make it unsuitable as an implant. Moreover, such a scaffold can produce acidic degradations that cause an inflammatory response at the implantation site. To overcome these negative aspects, composite electrospun nanofiber scaffolds of PCL/oyster shell (OS) were developed in this study. Then, surface morphology and chemistry of the given scaffolds were characterized. As well, tensile strength and surface hydrophilicity were evaluated. The osteogenic proliferation and differentiation potentials of these scaffolds were further evaluated through determining basic osteogenic markers using human adipose tissue derived from mesenchymal stem cells. The results revealed that the scaffolds fabricated had a very good surface property and better tensile strength than pristine PCL ones. The osteogenic proliferation and differentiation potentials were also reported to be better than those in pristine PCL scaffolds. Hence, the presence of OS could enhance PCL surface properties and bioactivity. Therefore, PCL/OS composite scaffolds developed in this study were assumed cost-effective and ideal which would offer promising alternatives for bone tissue engineering applications.
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References
Eshraghi S, Das S (2010) Mechanical and microstructural properties of polycaprolactone scaffolds with one-dimensional, two-dimensional, and three-dimensional orthogonally oriented porous architectures produced by selective laser sintering. Acta Biomater 6:2467–2476
Gomez-Lizarraga KK, Flores-Morales C, Del Prado-Audelo ML, Alvarez-Perez MA, Pina-Barba MC, Escobedo C (2017) Polycaprolactone-and polycaprolactone/ceramic-based 3D-bioplotted porous scaffolds for bone regeneration: a comparative study. Mater Sci Eng C 79:326–335
Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40:363–408
Birhanu G, Akbari Javar H, Seyedjafari E, Zandi-Karimi A, Dusti Telgerd M (2017) An improved surface for enhanced stem cell proliferation and osteogenic differentiation using electrospun composite PLLA/P123 scaffold. Artif Cell Nanomed 46:1–8
Elangomannan S, Louis K, Dharmaraj BM, Saravanan Kandasamy V, Soundarapandian K, Gopi D (2017) Carbon nanofiber/polycaprolactone/mineralized hydroxyapatite nanofibrous scaffolds for potential orthopedic applications. ACS Appl Mater Interfaces 9:6342–6355
Langer R, Vacanti JP, Vacanti CA, Atala A, Freed LE, Vunjak-Novakovic G (1995) Tissue engineering: biomedical applications. Tissue Eng 1:151–161
Vacanti JP, Langer R (1999) Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet 354:S32–S34
Shrivats AR, McDermott MC, Hollinger JO (2014) Bone tissue engineering: state of the union. Drug Discov Today 19:781–786
Ahmadi M, Seyedjafari E, Zargar SJ, Birhanu G, Zandi-Karimi A, Beiki B, Tuzlakoglu K (2017) Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton’s Jelly. EXCLI J 16:785
Motamedian SR, Hosseinpour S, Ahsaie MG, Khojasteh A (2015) Smart scaffolds in bone tissue engineering: a systematic review of literature. World J Stem Cells 7:657
Kim Y, Kim G (2015) Highly roughened polycaprolactone surfaces using oxygen plasma-etching and in vitro mineralization for bone tissue regeneration: fabrication, characterization, and cellular activities. Colloids Surf B Biointerfaces 125:181–189
Rath SN, Pryymachuk G, Bleiziffer OA, Lam ChXF, Arkudas A, Ho STB, Beier JP, Horch RE, Hutmacher DW, Kneser U (2011) Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: in vitro and in vivo evaluation in a PLDLLA–TCP–PCL-composite system. J Mater Sci Mater Med 22:1279–1291
Temple JP, Hutton DL, Hung BP, Yilgor Huri P, Cook CA, Kondragunta R, Jia X, Grayson WL (2014) Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds. J Biomed Mater Res B 102:4317–4325
Prabhakaran MP, Venugopal JR, Chyan TT, Beng Hai L, Chan CK, Yutang Lim A, Ramakrishna S (2008) Electrospun biocomposite nanofibrous scaffolds for neural tissue engineering. Tissue Eng Part A 14:1787–1797
Cipitria A, Skelton A, Dargaville TR, Dalton PD, Hutmacher DW (2011) Design, fabrication and characterization of PCL electrospun scaffolds—a review. J Mater Chem 21:9419–9453
Melchels FPW, Barradas AM, Van Blitterswijk CA, de Boer J, Feijen J, Grijpma DW (2010) Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing. Acta Biomater 6:4208–4217
Park JS, Lee SJ, Jo HH, Lee JH, Kim WD, Lee JY, Park SA (2017) Fabrication and characterization of 3D-printed bone-like β-tricalcium phosphate/polycaprolactone scaffolds for dental tissue engineering. Ind Eng Chem Res 46:175–181
Wu H, Wan Y, Dalai S, Zhang R (2010) Response of rat osteoblasts to polycaprolactone/chitosan blend porous scaffolds. J Biomed Mater Res B 92:238–245
Ho CC, Fang HY, Wang B, Huang TH, Shie MY (2018) The effects of Biodentine/polycaprolactone 3D-scaffold with odontogenesis properties on human dental pulp cells. Int Endod J 51:291–300
Kim MS, Kim G (2014) Three-dimensional electrospun polycaprolactone (PCL)/alginate hybrid composite scaffolds. Carbohydr Polym 114:213–221
Mirhosseini MM, Haddadi‐Asl V, Zargarian SS (2016) Fabrication and characterization of hydrophilic poly (ε‐caprolactone)/pluronic P123 electrospun fibers. J Appl Polym Sci 133:43345–43356
Ghaee A, Nourmohammadi J, Danesh P (2017) Novel chitosan-sulfonated chitosan-polycaprolactone-calcium phosphate nanocomposite scaffold. Carbohydr Polym 157:695–703
Shin SH, Purevdorj O, Castano O, Planell JA, Kim H-W (2012) A short review: recent advances in electrospinning for bone tissue regeneration. Tissue Eng. 3:2041731412443530
Díaz E, Sandonis I, Valle MB (2014) In vitro degradation of poly (caprolactone)/nHA composites. J Nanomater 185:1–8
Chakrapani VY, Kumar TS, Raj DK, Kumary TV (2017) Electrospun 3D composite scaffolds for craniofacial critical size defects. J Mater Sci Mater Med 28:119
Liu Z, Ji J, Tang S, Qian J, Yan Y, Yu B, Su J, Wei J (2015) Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly (L-lactide) composite. J R Soc Interface 12:1–11
Vecchio KS, Zhang X, Massie JB, Wang M, Kim ChW (2007) Conversion of bulk seashells to biocompatible hydroxyapatite for bone implants. Acta Biomater 3:910–918
Lee YK, Jung SK, Chang YH, Wang M, Kim CW (2017) Highly bioavailable nanocalcium from oyster shell for preventing osteoporosis in rats. Int J Food Sci Nutr 68:1–10
Yoon GL, Kim BT, Kim BO, Han S-H (2003) Chemical–mechanical characteristics of crushed oyster-shell. Waste Manag 23:825–834
Wu SC, Hsu HC, Wu YN, Ho W-F (2011) Hydroxyapatite synthesized from oyster shell powders by ball milling and heat treatment. Mater Charact 62:1180–1187
Fan L, Zhang S, Zhang X, Zhou H, Lu Z, Wang S (2015) Removal of arsenic from simulation wastewater using nano-iron/oyster shell composites. J Environ Manag 156:109–114
Shen Y, Yang S, Liu J, Xu H, Shi Zh, Lin Zh, Ying X, Guo P, Lin T, Yan Sh, Huang Q, Peng L (2014) Engineering scaffolds integrated with calcium sulfate and oyster shell for enhanced bone tissue regeneration. ACS Appl Mater Interfaces 6:12177–12188
Yang Y, Yao Q, Pu X, Hou Zh, Zhang Q (2011) Biphasic calcium phosphate macroporous scaffolds derived from oyster shells for bone tissue engineering. Chem Eng 173:837–845
Kazemi SY, Biparva P, Ashtiani E (2016) Cerastoderma lamarcki shell as a natural, low cost and new adsorbent to removal of dye pollutant from aqueous solutions: equilibrium and kinetic studies. Ecol Eng 88:82–89
Xue R, Qian Y, Li L, Yao G, Yang L, Sun Y (2017) Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells. Stem Cell Res Ther 8:148
Yu Y, Hua S, Yang M, Fu Z, Teng S, Niu K, Zhao Q, Yi Ch (2016) Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold. RSC Adv. 6:110557–110565
Mohammad Ghorbani F, Kaffashi B, Shokrollahi P, Seyedjafari E, Ardeshirylajimi A (2015) PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation. Carbohydr Polym. 118:133–142
Amjadian S, Seyedjafari E, Zeynali B, Shabani I (2016) The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly (l-lactide) on the osteogenesis of mesenchymal stem cells. Int J Pharm 507:1–11
Miller ND, Williams DF (1987) On the biodegradation of poly-β-hydroxybutyrate (PHB) homopolymer and poly-β-hydroxybutyrate-hydroxyvalerate copolymers. Biomaterials 8:129–137
Mouriès LP, Almeida MJ, Milet Ch, Berland S, Lopez E (2002) Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk Pinctada maxima in three mammalian cell types: fibroblasts, bone marrow stromal cells and osteoblasts. Comp Biochem Physiol B Biochem Mol Biol 132:217–229
Combes C, Miao B, Bareille R, Rey Ch (2006) Preparation, physical–chemical characterisation and cytocompatibility of calcium carbonate cements. Biomaterials 27:1945–1954
Lamghari M, Almeid MJ, Berland S, Huet H, Laurent A, Milet C, Lopez E (1999) Stimulation of bone marrow cells and bone formation by nacre: in vivo and in vitro studies. Bone 25:91S–94S
Lamghari M, Berland S, Laurent A, Huet H, Lopez E (2001) Bone reactions to nacre injected percutaneously into the vertebrae of sheep. Biomaterials 22:555–562
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Didekhani, R., Sohrabi, M.R., Soleimani, M. et al. Incorporating PCL nanofibers with oyster shell to improve osteogenic differentiation of mesenchymal stem cells. Polym. Bull. 77, 701–715 (2020). https://doi.org/10.1007/s00289-019-02750-x
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DOI: https://doi.org/10.1007/s00289-019-02750-x