Controlled release profile of 5-fluorouracil loaded P(AAM-co-NVP-co-DEAEMA) microgel prepared via free radical precipitation polymerization
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A new microgel was prepared by free radical precipitation polymerization of acrylamide (AAM), 1-vinyl-2-pyrrolidone (NVP) and 2-(diethylamino)ethyl methacrylate (DEAEMA) to be used for the controlled drug release. The zeta-potential and particle size of P(AAM-co-NVP-co-DEAEMA) microgel were measured by dynamic light scattering (DLS) measurements and found to be around − 36.4 ± 6.29 mV and 592 nm, respectively. P(AAM-co-NVP-co-DEAEMA) microgel was loaded with 5-Fluorouracil (5-Fu) as a model drug. In vitro drug release profiles demonstrated that 5-Fu release from P(AAM-co-NVP-co-DEAEMA) microgel at pH 7.4 was much faster than at pH 5.5 and pH 2.1. In addition, release kinetic studies showed that P(AAM-co-NVP-co-DEAEMA) microgel fit into Higuchi model release. The cytotoxicity study indicated that P(AAM-co-NVP-co-DEAEMA) microgel did not exhibit apparent cytotoxicity against L929 cell line. When all of the results were evaluated, it can be seen that P(AAM-co-NVP-co-DEAEMA) microgel can be effectively used as a polymeric drug carrier for the potential drug delivery systems in medical applications.
Keywords2-(Diethylamino)ethyl methacrylate Microgel Precipitation polymerization Controlled release 5-Fluorouracil
The authors wish to thank Prof. Dr. Gamze Güçlü, Assoc. Prof. Dr. Işıl Acar and Assoc. Prof. Dr. Serkan Emik for their support. The present work was financially supported by the Research Fund of the Hitit University, Project no. MUH19002.14.004.
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