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Isolation and characterization of galactomannan from seed of Leucaena leucocephala

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Abstract

Seeds from Leucaena leucocephala or commonly known as white leadtree are rich in galactomannan, a reserved polysaccharide composed of d-mannose and d-galactose residues. Galactomannan was isolated from the mature seeds of L. leucocephala using water extraction followed by precipitation with ethanol and purified using ion-exchange chromatography. Pure galactomannan was successfully isolated with a yield of 50.31% (w/w). It was chemically analyzed by UV, NMR, FT-IR, GPC, and HPLC. The polysaccharide was further hydrolyzed and derivatized with 3-methyl-1-phenyl-5-pyrazolone (PMP) for determination of its monosaccharide composition using a pre-column derivatization HPLC–UV technique. It consisted of d-mannose and d-galactose with mannose:galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. FT-IR analysis, 1H, and 13C NMR of the galactomannan produced are similar to those reported in the literatures.

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Acknowledgements

The authors thank the Forest Research Institute Malaysia (FRIM) and Ministry of Science, Technology and Innovation Malaysia (MOSTI), for generous financial support under project Grant number 02-03-10-SF0091 to carry out this work.

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Authors and Affiliations

  1. Phytochemistry Program, Natural Products Divison, Forest Research Institute Malaysia (FRIM), 52109, Kepong, Selangor, Malaysia

    Nurnadiah Rahim, Abd Rashid Li & Mohd Radzi Ahmad

  2. Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia

    Nurnadiah Rahim & Dzaraini Kamarun

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  1. Nurnadiah Rahim
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Correspondence to Nurnadiah Rahim.

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Rahim, N., Li, A.R., Kamarun, D. et al. Isolation and characterization of galactomannan from seed of Leucaena leucocephala . Polym. Bull. 75, 2027–2037 (2018). https://doi.org/10.1007/s00289-017-2135-7

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  • DOI: https://doi.org/10.1007/s00289-017-2135-7

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