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A novel biosensor based on electro-co-deposition of sodium alginate-Fe3O4-graphene composite on the carbon ionic liquid electrode for the direct electrochemistry and electrocatalysis of myoglobin

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Abstract

A novel biosensor based on electro-co-deposition of myoglobin (Mb), sodium alginate (SA), Fe3O4-graphene (Fe3O4-GR) composite on the carbon ionic liquid electrode (CILE) was fabricated using Nafion as the film forming material to improve the stability of protein immobilized on the electrode surface, and the modified electrode was abbreviated as Nafion/Mb-SA-Fe3O4-GR/CILE. FT-IR and UV–vis absorption spectra suggested that Mb could retain its native structure after being immobilized in the SA-Fe3O4-GR composite film. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry, and a pair of symmetric redox peaks appeared in the cyclic voltammograms, indicating that direct electron transfer of Mb was realized on the modified electrode, which was ascribed to the good electrocatalytic capability of Fe3O4-GR composite, the good biocompatibility of SA and the synergistic effects of SA and Fe3O4-GR composite. The electrochemical parameters of the electron transfer number (n), the charge transfer coefficient (α) and the electron transfer rate constant (k s) were calculated as 0.982, 0.357 and 0.234 s−1, respectively. The modified electrode exhibited good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) with wide linear range from 1.4 to 119.4 mmol/L, low detection limit as 0.174 mmol/L (3σ), good stability and reproducibility.

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Acknowledgments

We gratefully thank the financial support form the National Natural Science Foundation of China (21366010, 21566009) and Key Projects in the Hainan provincial Science & Technology Program (ZDXM2014037).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571100, Hainan, People’s Republic of China

    Xiuqiong Chen, Huiqiong Yan, Zaifeng Shi, Qiang Lin, Xianghui Wang & Wei Sun

  2. College of Materials and Chemical Engineering, Hainan University, Haikou, 570228, People’s Republic of China

    Yuhong Feng & Jiacheng Li

Authors
  1. Xiuqiong Chen
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  2. Huiqiong Yan
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  3. Zaifeng Shi
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  4. Yuhong Feng
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  5. Jiacheng Li
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  6. Qiang Lin
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  7. Xianghui Wang
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  8. Wei Sun
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Correspondence to Qiang Lin or Wei Sun.

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X. Chen and H. Yan are co-first authors.

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Chen, X., Yan, H., Shi, Z. et al. A novel biosensor based on electro-co-deposition of sodium alginate-Fe3O4-graphene composite on the carbon ionic liquid electrode for the direct electrochemistry and electrocatalysis of myoglobin. Polym. Bull. 74, 75–90 (2017). https://doi.org/10.1007/s00289-016-1698-z

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  • DOI: https://doi.org/10.1007/s00289-016-1698-z

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