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β-Chitin/gelatin/nanohydroxyapatite composite scaffold prepared through freeze-drying method for tissue engineering applications

Abstract

The nanocomposite scaffolds were synthesized using freeze-drying method by blending β-chitin hydrogel (CT), gelatin (GE) and nanohydroxyapatite (nHAp) in the inorganic/organic different weight ratio. The nHAp was developed using calcium nitrate and diammonium hydrogenphosphate as the precursors of inorganic phase. The prepared nHAp and composite scaffolds were characterized using BET, SEM, FT-IR, XRD, and TGA studies. The composite scaffolds were found to have 70–76 % porosity with well-defined interconnected porous structure. Swelling studies of CT and CT/GE/nHAp composite scaffolds presented high swelling. The prepared scaffold materials are biodegradability and show less than 20 % mass loss after 4 weeks incubation in a phosphate-buffered saline (PBS) medium containing lysozyme (10,000 U/mL) at 37 °C. Moreover, the cell viability, adhesion and rapid growth using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and mouse preosteoblast cell implied the cytocompatibility nature of the composite scaffolds. These results prove that this material can be an ability to be a candidate for tissue engineering applications.

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Acknowledgments

Supports from Payame Noor University in Isfahan Research Council (Grant No. 84910) and contribution from Isfahan University of Technology are gratefully acknowledged.

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Correspondence to Abbas Teimouri.

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Teimouri, A., Azadi, M. β-Chitin/gelatin/nanohydroxyapatite composite scaffold prepared through freeze-drying method for tissue engineering applications. Polym. Bull. 73, 3513–3529 (2016). https://doi.org/10.1007/s00289-016-1691-6

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  • DOI: https://doi.org/10.1007/s00289-016-1691-6

Keywords

  • Chitin
  • Gelatin
  • Nanohydroxyapatite
  • Composite scaffold
  • Tissue engineering