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Osteogenic activities of polymeric soybean oil-g-polystyrene membranes

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Abstract

A novel biocompatible copolymer membrane was synthesized and characterized for use in guided bone regeneration using polymeric soybean oil-g-polystyrene (PSO-g-PS) graft copolymer which was successfully obtained by free radical polymerization of styrene initiated by PSO peroxide as a macroinitiator at 80 °C. Osteoblastic cellular activities of MC3T3-E1 cells on PSO-g-PS membranes with different soybean oil composition (PSO-g-PS1, PSO-g-PS2, and PSO-g-PS3) were evaluated. Nuclear magnetic resonance (1H NMR) spectra showed that PSO inclusion (mol%) was found to be 27, 69, and 51 % for PSO-g-PS1, PSO-g-PS2, and PSO-g-PS3 membranes, respectively. Superior biocompatibility of the PSO-g-PS membranes was determined compared to polystyrene tissue culture plates (TCPS) as positive control. Cell proliferation was enhanced on PSO-g-PS2 and PSO-g-PS3 membranes compared to PSO-g-PS1 membranes (p < 0.001), and a statistically significant higher ALP value of MC3T3-E1 cells on PSO-g-PS2 membranes (p < 0.05) suggested that proliferation and differentiation of preosteoblastic on PSO-g-PS membranes were enhanced with regard to soybean oil content within the membranes. Thus, the present study suggests that PSO-g-PS2 membranes, which showed a favorable biological environment for the preosteoblastic cells, can be well suited for bone tissue engineering applications.

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Acknowledgments

The Authors thank to TÜBİTAK (Grant No. 211T016) and Bülent Ecevit University (Grant# 2011-10-3-02) for financial support.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Bülent Ecevit University, Zonguldak, 67100, Turkey

    R. Seda Tığlı Aydın

  2. Department of Chemistry, Bülent Ecevit University, 67100, Zonguldak, Turkey

    Baki Hazer & Merve Acar

  3. Department of Chemical Engineering, Hacettepe University, 06530, Beytepe/Ankara, Turkey

    Menemşe Gümüşderelioğlu

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  1. R. Seda Tığlı Aydın
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Correspondence to R. Seda Tığlı Aydın or Baki Hazer.

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Tığlı Aydın, R.S., Hazer, B., Acar, M. et al. Osteogenic activities of polymeric soybean oil-g-polystyrene membranes. Polym. Bull. 70, 2065–2082 (2013). https://doi.org/10.1007/s00289-013-0976-2

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  • DOI: https://doi.org/10.1007/s00289-013-0976-2

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