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Preparation of avidin-containing polyelectrolyte microcapsules and their uptake and release properties

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Abstract

Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules. Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution due to the competitive binding of the additives to the binding site of avidin.

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Acknowledgment

The present study was supported in part by Grant-in-Aid (21·7075) from the Japan Society for the Promotion of Sciences (JSPS).

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai, 980-8578, Japan

    Yoshihiro Endo, Katsuhiko Sato & Jun-ichi Anzai

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  1. Yoshihiro Endo
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  2. Katsuhiko Sato
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  3. Jun-ichi Anzai
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Correspondence to Jun-ichi Anzai.

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Endo, Y., Sato, K. & Anzai, Ji. Preparation of avidin-containing polyelectrolyte microcapsules and their uptake and release properties. Polym. Bull. 66, 711–720 (2011). https://doi.org/10.1007/s00289-010-0375-x

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  • DOI: https://doi.org/10.1007/s00289-010-0375-x

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