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Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions

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Abstract

Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (up to 1.3 mmol/g). The potential use of immobilized Ni(II)-IDA complexes for sorption of murine immunoglobulin G by the immobilized metal affinity (IMA) method was tested. The sorption behaviour was characterized by a Langmuir-Freundlich adsorption isotherm. It was shown that the chelating membranes could be used for the sorption of low amounts of protein.

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References

  1. Singh M, Srinivasan KV (2002) Chromatographia 56:717–722

    Article  CAS  Google Scholar 

  2. Beauvais RA, Alexandratos SD (1998) React Funct Polym 36:113–123

    Article  CAS  Google Scholar 

  3. Alieva RA, Gamidov SZ, Chyragov FM, Azizov AA (2005) J Anal Chem 60:1114–1117

    Article  CAS  Google Scholar 

  4. Do L, Smith RC, Tennyson AG, Lippard SJ (2006) Inorg Chem 45:8998–9005

    Article  CAS  Google Scholar 

  5. Hrubý M, Hradil J, Beneš MJ (2004) React Funct Polym 59:105–118

    Article  Google Scholar 

  6. Porath J, Carlsson J, Olsson I, Belfrage G (1975) Nature 258:598–599

    Article  CAS  Google Scholar 

  7. Gomez CG, Alvarez Igarzabal CI, Strumia MC (2005) Polymer 46:6300–6307

    Article  CAS  Google Scholar 

  8. Emir S, Say R, Yavuz H, Denizli A (2004) Biotechnol Prog 20:223–228

    Article  CAS  Google Scholar 

  9. Denizli A, Garipcan B, Emir S, Patir S, Say R (2002) Adsorption Sci Technol 20:607–617

    Article  CAS  Google Scholar 

  10. Garipcan B, Andac M, Uzun L, Denizli A (2004) React Funct Polym 59:119–128

    Article  CAS  Google Scholar 

  11. Denizli A, Senel S, Arica MY (1998) Colloid Surf B 11:113–122

    Article  CAS  Google Scholar 

  12. Arica MY, Testereci HN, Denizli A (1998) J Chromatogr A 799:83–91

    Article  CAS  Google Scholar 

  13. Hale JE, Beidler DE (1994) Anal Biochem 222:29–33

    Article  CAS  Google Scholar 

  14. Kopeèek J, Jokl J, Lím D (1968) J Polym Sci C 16:3877–3889

    Google Scholar 

  15. Gomez CG, Alvarez CI, Strumia MC, Rivas BL, Reyes P (2001) J Appl Polym Sci 79:920–927

    Article  CAS  Google Scholar 

  16. Warburg O, Christian W (1941) Biochem Z 310:384–421

    Google Scholar 

  17. Kopeèek J, Lím D (1971) Collect Czech Chem Commun 36:2703–2707

    Google Scholar 

  18. Gatenholm P, Michálek J, Vacík J (1996) Macromol Symp 109:127–133

    CAS  Google Scholar 

  19. Karlsson JO, Gatenholm P (1996) Polymer 37:4251–4256

    Article  CAS  Google Scholar 

  20. Mészárosová K, Tishchenko G, Bouchal K, Bleha M (2003) React Funct Polym 56:27–35

    Article  Google Scholar 

  21. Shukla SR, Pai RS, Shendarkar AD (2006) Sep Purif Technol 47:141–147

    Article  CAS  Google Scholar 

  22. Pearson RG (1990) Coord Chem Rev 100:403–425

    Article  CAS  Google Scholar 

  23. Serpa G, Augusto EFP, Tamashiro WMSC, Ribeiro MB, Miranda EA, Bueno SMA (2005) J Chromatogr B 816:259–268

    Article  CAS  Google Scholar 

  24. Sharma S, Agarwal GP (2001) Anal Biochem 288:126–140

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovsky Sq. 2, 162 06, Prague 6, Czech Republic

    Kateřina Kráčalíková & Miroslav Bleha

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  1. Kateřina Kráčalíková
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  2. Miroslav Bleha
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Correspondence to Kateřina Kráčalíková.

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Kráčalíková, K., Bleha, M. Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions . Polym. Bull. 61, 147–156 (2008). https://doi.org/10.1007/s00289-008-0940-8

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  • DOI: https://doi.org/10.1007/s00289-008-0940-8

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