Abstract.
Rapid growth of Salmonella typhimurium LT2 in rich LB-broth caused the induction of the cysteine regulon when the culture reached an optical density at 650 nm of 1.5. Addition of 0.05 mM L-cystine to LB-broth abolished induction, while 0.025 mM L-cystine delayed it for a doubling time (30 min). Induction did not occur when lack of oxygen or a temperature shift to 19°C slowed down growth in LB-broth. Uninduced cultures reached growth yield similar to that of induced cultures after overnight incubation. Growth on solid LB-medium also brought about induction, but to a lower level than in liquid medium. Leaky cysB mutants, which are sensitive to the β-lactam antibiotic mecillinam, displayed partial induction, whereas mecillinam-resistant cysB and cysE mutants showed no induction. It is suggested that induction develops in these mutants when constitutive transport systems fail to satisfy the high uptake of cysteine demanded by fast-growing cultures. The behavior of cysB mutants agrees with the hypothesis that, under some conditions, mecillinam action would be dependent on expression of the cysteine regulon.
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Received: 9 June 1999 / Accepted: 11 August 1999
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Antón, D. Induction of the Cysteine Regulon of Salmonella typhimurium in LB Medium Affects the Response of cysB Mutants to Mecillinam. Curr Microbiol 40, 72–77 (2000). https://doi.org/10.1007/s002849910014
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DOI: https://doi.org/10.1007/s002849910014