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Cloning of Schizosaccharomyces pombe bio2 by Heterologous Complementation of a Saccharomyces cerevisiae Mutant

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Abstract.

A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterization of a novel bio gene: bio2, encoding biotin synthase from Schizosaccharomyces pombe.

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Authors and Affiliations

  1. Laboratoire de Biotechnologie, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brandt, 67400 Illkirch-Graffenstaden, France , , , , , , FR

    Vincent Phalip, Yves Lemoine, & Jean-Marc Jeltsch

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  1. Vincent Phalip
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  2. Yves Lemoine,
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  3. Jean-Marc Jeltsch
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Received: 4 June 1999 / Accepted: 12 July 1999

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Phalip, V., Lemoine,, Y. & Jeltsch, JM. Cloning of Schizosaccharomyces pombe bio2 by Heterologous Complementation of a Saccharomyces cerevisiae Mutant. Curr Microbiol 39, 348–350 (1999). https://doi.org/10.1007/s002849900470

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  • DOI: https://doi.org/10.1007/s002849900470

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