Abstract.
The Azospirillum brasilense trpE gene has been isolated by DNA hybridization and by genetic complementation of an Escherichia coli trpE deletion mutant. DNA sequence analysis of a 3.1-kb PstI restriction fragment of A. brasilense revealed the presence of an open reading frame encoding a putative TrpE(G) fusion protein. Previously an A. brasilense clone containing trpGDC was identified (Zimmer et al. Mol Gen Genet 229:41–51, 1991). It can, therefore, be concluded that A. brasilense contains two trpG genes. A putative leader peptide is found upstream of trpE(G), containing three consecutive tryptophan residues. Putative terminator and anti-terminator loops have also been identified. The LLESX10S motif, which is responsible for feedback inhibition by tryptophan in other TrpE proteins, is absent in the A. brasilense TrpE(G) fused protein.
Author information
Authors and Affiliations
Additional information
Received: 29 May 1996 / Accepted: 5 July 1996
Rights and permissions
About this article
Cite this article
De Troch, P., Dosselaere, F., Keijers, V. et al. Isolation and Characterization of the Azospirillum brasilense trpE(G) Gene, Encoding Anthranilate Synthase . Curr Microbiol 34 , 27 –32 (1997). https://doi.org/10.1007/s002849900139
Issue Date:
DOI: https://doi.org/10.1007/s002849900139