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Differential Induction of the Chaperonin GroEL and the Co-Chaperonin GroES by Heat, Acid, and UV-Irradiation in Lactococcus lactis subsp. lactis

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Abstract.

Microsequencing of a polypeptide with MW of 14.5 and pI of 5.0 induced by heat treatment at 42°C and 50°C in Lactococcus lactis subsp. lactis revealed that it corresponds to the co-chaperonin GroES. Quantitative analysis of analytical 2-D gels showed a relative induction of 12- and 11-fold after 30 min of heat adaptation at 42°C and 50°C, respectively. GroES is also induced by an acid shift from pH 7 to pH 5.5 and by UV254 nm-irradiation, with relative induction factors of 3.8 and 2.3, respectively. To our knowledge this is the first report showing induction of GroES by mild acid treatment. Contrasting to the relative induction of the groEL gene product, the second protein encoded by the groESL operon, GroES shows significantly higher induction under all stress situations.

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Authors and Affiliations

  1. Laboratoire de Microbiologie de l’Environnement, Université de Caen, F-14032 Caen Cedex, France , , , , , , FR

    Axel Hartke, Jacques Frère, Philippe Boutibonnes & Yanick Auffray

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  1. Axel Hartke
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  2. Jacques Frère
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  3. Philippe Boutibonnes
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  4. Yanick Auffray
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Received: 3 June 1996 / Accepted: 5 July 1996

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Hartke, A., Frère, J., Boutibonnes, P. et al. Differential Induction of the Chaperonin GroEL and the Co-Chaperonin GroES by Heat, Acid, and UV-Irradiation in Lactococcus lactis subsp. lactis . Curr Microbiol 34 , 23 –26 (1997). https://doi.org/10.1007/s002849900138

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  • DOI: https://doi.org/10.1007/s002849900138

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