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Behavior of Variable V3 Region from 16S rDNA of Lactic Acid Bacteria in Denaturing Gradient Gel Electrophoresis

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Abstract

Separation of amplified V3 region from 16S rDNA by denaturing gradient gel electrophoresis (DGGE) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. Variable V3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera Pediococcus, Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, Weissella, and Streptococcus were analyzed. DGGE profiles obtained were species-specific for most of the cultures tested. Moreover, it was possible to group the remaining LAB reference strains according to the migration of their 16S V3 region in the denaturing gel. The results are discussed with reference to their potential in the analysis of LAB communities in food, besides shedding light on taxonomic aspects.

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Authors and Affiliations

  1. Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare, Ambientale e di Igiene, Stazione di Microbiologia Industriale, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Italy, IT

    Danilo Ercolini, Giancarlo Moschetti, Giuseppe Blaiotta & Salvatore Coppola

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  1. Danilo Ercolini
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  2. Giancarlo Moschetti
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  3. Giuseppe Blaiotta
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  4. Salvatore Coppola
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Received: 2 August 2000 / Accepted: 5 September 2000

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Ercolini, D., Moschetti, G., Blaiotta, G. et al. Behavior of Variable V3 Region from 16S rDNA of Lactic Acid Bacteria in Denaturing Gradient Gel Electrophoresis. Curr Microbiol 42, 199–202 (2001). https://doi.org/10.1007/s002840010204

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  • DOI: https://doi.org/10.1007/s002840010204

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