Abstract
The region encompassing the α-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835T by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,909. The similarity of the deduced mature protein to other yeast α-galactosidases was 63.3% to Zygosaccharomyces cidri, 71.5% to Torulaspora delbrueckii, and 70.7–73.9% to Saccharomyces species. The nucleotide and amino acid sequences are deposited in the DDBJ/EMBL/GenBank database under Accession Number AB030209.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 10 February 2000 / Accepted: 29 March 2000
Rights and permissions
About this article
Cite this article
Oda, Y., Fujisawa, T. Nucleotide Sequence of α-Galactosidase MEL Gene from Zygosaccharomyces mrakii . Curr Microbiol 41, 220–222 (2000). https://doi.org/10.1007/s002840010123
Issue Date:
DOI: https://doi.org/10.1007/s002840010123