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Use of Ozone as a Substrate Treatment for the Control of Trichoderma in the Production of Pleurotus ostreatus

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Abstract

Pleurotus ostreatus is one of the most widely cultivated species in the world. It can be produced in many lignocellulosic substrates after carrying out a treatment to eliminate competing microorganisms. The most commonly used is pasteurization by steam or by immersion in hot water. The aim of this work is to evaluate if ozone can be employed as treatment for decontamination of the substrate used for the production of the edible mushroom P. ostreatus to control of green mold Trichoderma. Wheat straw was employed as a substrate. We used two different methodologies: bubbling ozone into a tank with water and the substrate, and injecting ozone into a closed tank with the substrate inside. Ten treatments were carried out including two treatments with inoculation by a spray of conidia of Trichoderma. The effect of ozone on the conidia was also evaluated. We found that the treatment of the substrate with ozone in immersed water resulted more effective (lower growth of Trichoderma) than injecting ozone into a closed tank. Anyway, we found that the contaminant fungi could grow on the substrate in both treatments with ozone. We observed that although ozone affected the conidia when it was bubbled into water, some of them still managed to survive and could germinate 72 h later. P. ostreatus could grow and produce fruiting bodies on a substrate that was previously treated with ozone and yields were not affected. Based on the results obtained, we conclude that ozone may not be an effective agent to control Trichoderma in highly contaminated substrates, at least in the experimental conditions that we used, for the production of P. ostreatus.

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Acknowledgements

This work was financed by Argentinian National Research Council (CONICET-PIP 1292), the National University of San Martín (UNSAM, Argentina).

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Authors and Affiliations

  1. Lab. of Mycology and Mushroom Cultivation, Instituto Tecnológico de Chascomús (CONICET-UNSAM), Chascomús, Provincia de Buenos Aires, Argentina

    Santiago Jaramillo Mejía, María B. Colavolpe, Mauricio Ganuza & Edgardo Albertó

  2. Escuela de Bio y Nanotecnologías (UNSAM), Buenos Aires, Argentina

    Santiago Jaramillo Mejía, María B. Colavolpe, Mauricio Ganuza & Edgardo Albertó

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  1. Santiago Jaramillo Mejía
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  2. María B. Colavolpe
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  3. Mauricio Ganuza
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  4. Edgardo Albertó
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Contributions

SJM and EA conceived and designed research. SJM and MC conducted experiments. SJM and MG contributed new reagents or analytical tools. SJM, MC, MG and EA analyzed data. SJM, MG, and EA wrote the manuscript.

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Correspondence to Edgardo Albertó.

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Jaramillo Mejía, S., Colavolpe, M.B., Ganuza, M. et al. Use of Ozone as a Substrate Treatment for the Control of Trichoderma in the Production of Pleurotus ostreatus. Curr Microbiol 81, 181 (2024). https://doi.org/10.1007/s00284-024-03691-z

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