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Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil

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Abstract

A novel Gram-stain-positive, oval-shaped, and non-flagellated bacterial strain YIM S02556T was isolated from forest soil in Xiongbi Town, Shizong County, Qujing City, Yunnan Province, southwestern China. The strain exhibited high pairwise 16 S rRNA gene sequence similarity with Psychromicrobium lacuslunae (97.3%) and Psychromicrobium silvestre (96.3%). Strain YIM S02556T exhibited an average nucleotide identity (ANI) of 72.5% with P. lacuslunae IHBB 11,108T and 72.8% ANI with P. silvestre AK 20-18T. The digital DNA-DNA hybridization (dDDH) value between strain YIM S02556T and P. lacuslunae IHBB 11,108T was 20.2%, while with P. silvestre AK 20-18T, the dDDH value was 20.8%. Strain YIM S02556T exhibited optimal growth at 28 °C, pH 7.0, without NaCl. Growth occurred within 10–37 ℃, pH 5.0–8.0, and in the presence of up to 5% w/v NaCl concentration. The genome size was 3.1 Mbp with 64.2% G + C content. The predominant menaquinone was MK-8(H4). The major cellular fatty acid was anteiso-C15:0. Based on the polyphasic analysis, strain YIM S02556T (= KCTC 49,805T = CCTCC AB2020166T) represents a novel Psychromicrobium species in which the name Psychromicrobium xiongbiense sp.nov. was proposed.

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Acknowledgements

The authors gratefully acknowledge the invaluable comments of Professor Aharon Oren from the Hebrew University of Jerusalem, Israel; Professor Bernhard Schink from the University of Konstanz, Germany; Professor Stefano Ventura from CNR-IRET UOS Firenze, Italy; and Professor Marko Kostovski from University Ss Cyril and Methodius, North Macedonia in providing nomenclature review. The authors are also indebted to Dr Yupeng Liang from China for insightful discussions on the phylogenetic placement of YIM S02556T. Furthermore, special thanks go to Dr Guiding Li for their exceptional technical assistance.

Funding

This work was supported by Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities’ Association (Grant NO. 202301BA070001-081, NO. 2018FH001-040), Yunnan Fundamental Research Projects (Grant NO. 202301AU070012, Grant NO. 202301AU070015), Special Basic Cooperative Research Innovation Programs of Qujing Science and Technology Bureau & Qujing Normal University (Grant NO. KJLH2022YB07, Grant NO. KJLH2023ZD06).

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Authors and Affiliations

  1. Yunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing, Yunnan, 655011, People’s Republic of China

    Ling Zhu, Man Zhu, Xiu Gao & Jian Cai

  2. School of Chemistry Biology and Environment, Yuxi Normal University, Yuxi, Yunnan, 653100, People’s Republic of China

    Yu-Chen Wang

  3. Department of Medicine, Qujing Vocational and Technical College, Qujing, Yunnan, 655000, People’s Republic of China

    Le-Le Li

  4. School of Agronomy and Life Sciences, Zhaotong University, Zhaotong, Yunnan, 657000, People’s Republic of China

    Xian-Min Huang

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  1. Ling Zhu
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Contributions

The experiments were designed and conducted by L.Z. Data analysis was performed by L.Z., Y.C.W., L.L.L., M.X.H., M.Z., X.G., and J.C. The project was conceived and supervised by J.C. The paper was written by L.Z. and X.G. with contributions from all authors, in accordance with the approval of the manuscript.

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Correspondence to Xiu Gao or Jian Cai.

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Zhu, L., Wang, YC., Li, LL. et al. Psychromicrobium Xiongbiense sp. nov., an Actinobacterium Isolated from Forest Soil. Curr Microbiol 81, 99 (2024). https://doi.org/10.1007/s00284-024-03629-5

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