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Rhizosphere-Associated Anammox Bacterial Diversity and Abundance of Nitrogen Cycle-Related Functional Genes of Emergent Macrophytes in Eutrophic Wetlands

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Abstract

Rhizospheric microbial community of emergent macrophytes plays an important role in nitrogen removal, especially in the eutrophic wetlands. The objective of this study was to identify the differences in anammox bacterial community composition among different emergent macrophytes and investigate revealed the the main factors affecting on the composition, diversity, and abundance of anammox bacterial community. Results showed that the composition, diversity, and abundance of the anammox community were significantly different between the vegetated sediments of three emergent macrophytes and unvegetated sediment. The composition of the anammox bacterial community was different in the vegetated sediments of different emergent macrophytes. Also, the abundance of nitrogen cycle-related functional genes in the vegetated sediments was found to be higher than that in the unvegetated sediment. Canonical correspondence analysis (CCA) and structural equation models analysis (SEM) showed that salinity and pH were the main environmental factors influencing the composition and diversity of the anammox bacterial community and NO2−-N indirectly affected anammox bacterial community diversity by affecting TOC. nirK-type denitrifying bacteria abundance had significant effects on the bacterial community composition, diversity, and abundance of anammox bacteria. The community composition of anammox bacteria varies with emergent macrophyte species. The rhizosphere of emergent macrophytes provides a favorable environment and promotes the growth of nitrogen cycling-related microorganisms that likely accelerate nitrogen removal in eutrophic wetlands.

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All data generated or analyzed during this study are included in this published article (and its supplementary information files).

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Funding

This study was funded by the Science and Technology Major Project on Lakes of Inner Mongolia grant (ZDZX2018054), National Natural Science Foundation of China grants (32160028).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia University, 235 West University Street, Hohhot, 010021, China

    Shaohua Zhang, Duo Zhang, Ying Guo, Ji Zhao & Zhihua Bao

  2. Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource Reuse, Inner Mongolia University, Hohhot, 010021, China

    Ji Zhao & Zhihua Bao

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  1. Shaohua Zhang
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  2. Duo Zhang
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  3. Ying Guo
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Contributions

ZSH: Conceptualization, Investigation, Formal analysis, Writing—original draft, Writing—review & editing. ZD: Investigation, Data analysis. GY: Investigation, Data analysis. ZJ: Conceptualization, Investigation, Writing—review&editing. BZH: Conceptualization, Investigation, Formal analysis, Writing—original draft, Writing—review & editing.

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Correspondence to Shaohua Zhang.

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High-quality anammox hzsB genes high-throughput sequencing data were submitted to GenBank (accession number: SRR12199323-SRR12199326)

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Zhang, S., Zhang, D., Guo, Y. et al. Rhizosphere-Associated Anammox Bacterial Diversity and Abundance of Nitrogen Cycle-Related Functional Genes of Emergent Macrophytes in Eutrophic Wetlands. Curr Microbiol 81, 107 (2024). https://doi.org/10.1007/s00284-024-03620-0

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