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Parendozoicomonas callyspongiae sp. nov. Isolated from a Marine Sponge, Callyspongia elongate, and Reclassification of Sansalvadorimonas verongulae as Parendozoicomonas verongulae comb. nov.

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Abstract

A strictly aerobic Gram-negative bacterium, designated 2012CJ34-2T, was isolated from marine sponge to Chuja-do in Jeju-island, Republic of Korea and taxonomically characterized. Cells were catalase- and oxidase-positive, and non-motile rods (without flagella). Growth was observed at 15–42 °C (optimum, 30 °C), pH 6–9 (optimum, pH 7), and in the presence of 0.5–10% (w/v) NaCl (optimum, 2–3%). The major cellular fatty acid and respiratory quinones were identified summed feature 3 (C16:1 ω7c/C16:1 ω6c), and Q-8 and Q-9, respectively. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified phospholipids, and three unidentified lipids. The DNA G+C content was 48.0 mol%. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences showed that strain 2012CJ34-2T formed a clade with Parendozoicomonas haliclonae S-B4-1UT and Sansalvadorimonas verongulae LMG 29871T within the family Endozoicomodaceae. Genome relatedness values, including dDDH, ANI and AF, and AAI and POCP, among strain 2012CJ34-2T, P. haliclonae S-B4-1UT, and S. verongulae LMG 29871T were within the range of the bacterial genus cut-off values. Based on the phylogenetic, chemotaxonomic, and genomic analyses, strain 2012CJ34-2T represents a novel bacterial species of the family Endozoicomodaceae, for which the name Parendozoicomonas callyspongiae sp. nov. is proposed. The type strain is 2012CJ34-2T (= KACC 22641T = LMG 32581T). Additionally, we proposed the reclassification of Sansalvadorimonas verongulae of the family Hahellaceae as Parendozoicomonas verongulae of the family Endozoicomonadaceae.

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Fig. 1
Fig. 2

Abbreviations

ML:

Maximum-likelihood

NJ:

Neighbor-joining

MP:

Maximum-parsimony

Q:

Ubiquinone

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

AAI:

Average amino acid identity

POCP:

Percentage of conserved proteins

AF:

Alignment fraction

MA:

Marine agar

MB:

Marine broth

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Acknowledgements

This research was supported by the management of Marine Fishery Bio-resources Center (2023) funded by the National Marine Biodiversity Institute of Korea (MABIK), and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A3046479). We gratefully thank Professor (Emeritus) Aharon Oren, The Hebrew University of Jerusalem, for reviewing nomenclature for new names of bacterial species.

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  1. Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-dong, Yuseong-gu, Daejeon, 34430, Republic of Korea

    Soo-Bin Kim, Kyung Hyun Kim & Jin-Sook Park

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Contributions

JSP conceived the ideas and supervised all works. SBK collected the samples, isolated the strain, and performed initial cultivation, storage, and deposition, and sequenced and analyzed the genome. SBK and KHK analyzed the phenotypic, biochemical, and genomic properties. SBK and JSP wrote the manuscript, and the manuscript has been reviewed and edited by all authors.

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Correspondence to Jin-Sook Park.

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The GenBank accession number for the 16S rRNA gene and whole genome sequences of strain 2012CJ34-2T are OM647832 and JAMFLX000000000, respectively.

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Kim, SB., Kim, K.H. & Park, JS. Parendozoicomonas callyspongiae sp. nov. Isolated from a Marine Sponge, Callyspongia elongate, and Reclassification of Sansalvadorimonas verongulae as Parendozoicomonas verongulae comb. nov.. Curr Microbiol 81, 85 (2024). https://doi.org/10.1007/s00284-023-03585-6

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