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Assessment of Antimicrobial and Antioxidant Potential of Oscillatoria sancta and Oscillatoria proteus Isolated from Chilika Lake

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Abstract

Filamentous cyanobacteria are a promising source of biologically active secondary metabolites with antioxidant, antimicrobial, antiviral and anticancer properties. Previously, cyanobacteria isolated from fresh and marine water were studied extensively, but those isolated from brackish water were less investigated. The purpose of this study was to examine the antimicrobial activities as well as the potential antioxidant capacity of two cyanobacterial strains (Oscillatoria proteus and Oscillatoria sancta) obtained from Chilika Lake. The pigment and antioxidant was assayed using a spectrophotometer; antimicrobial activity was studied by minimum inhibitory concentration (MIC); and the presence of phytoconstituents was detected using gas chromatography mass spectrometry (GC-MS). The solvents used for extraction were methanol, acetone and benzene. The experimental data indicates that the total phenolic and flavonoid content was highest in O. sancta (58.26 ± 0.72 µg/g, 38.45 ± 0.79 µg/g, respectively). Similarly, the methanol extract of O. sancta presented the maximum antioxidant potential in both DPPH (83.18 ± 0.57%) and ABTS (68.42 ± 1.40%) radicals. Besides, more reducing power was also recorded in methanol extract of O. sancta as compared to O. proteus. Further, higher enzymatic activity (superoxide dismutase and catalase) was observed in O. sancta. The antimicrobial potential against bacterial and fungal pathogens demonstrated better activity in O. sancta. In GC-MS analysis seven major chemical classes have been detected. Differential results was found in the two species of Oscillatoria; however, both have potential antimicrobial and antioxidant properties. The findings have pharmaceutical and nutraceutical importance.

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Acknowledgements

The authors extend their heartfelt thanks to Head, Department of Biotechnology, MSCB University, for providing laboratory facility to conduct the experiments.

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  1. Department of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Sriram Chandra Vihar, Takatpur, Baripada, Odisha, 757003, India

    Sabyasachy Parida, Satyabrata Dash, Bijayananda Sahoo & Biswajit Rath

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  1. Sabyasachy Parida
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Correspondence to Biswajit Rath.

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Parida, S., Dash, S., Sahoo, B. et al. Assessment of Antimicrobial and Antioxidant Potential of Oscillatoria sancta and Oscillatoria proteus Isolated from Chilika Lake. Curr Microbiol 81, 46 (2024). https://doi.org/10.1007/s00284-023-03563-y

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