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Effect of Entomopathogenic Fungi to Eggs and Nymphs Survival of Halyomorpha halys (Hemiptera: Pentatomidae) Under Laboratory Conditions

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Abstract

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is becoming a major pest of Greece’s agricultural production, including tree fruit. Until now, the control of this pest has been based on conventional methods, with the application of chemical insecticides being the primary option. However, the wide distribution of H. halys, in combination with the large area of tree fruit production in Greece, raise the need for alternative control methods. In this study, we investigated the possibility of implementing microbial control agents for managing H. halys. Eggs and nymphs of H. halys were treated with 15 native entomopathogenic fungal isolates and their virulence was evaluated in the laboratory. After treatment, egg hatching time was recorded for 7 days and ranged from 4.5 to 7.0 days. Nymphal survival was recorded daily for 7 days following application and ranged from 2.1 to 6.6 days for second instar nymphs and 3.7 to 6.8 days for fourth instar ones. Beauveria varroae isolate displayed the highest toxicity to all H. halys stages that were tested and could be considered a promising biocontrol agent of this insect. Please check and confirm that the authors and their respective affiliations have been correctly identified and amend if necessary.οκ

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Acknowledgements

This research was funded by the Operational Programme Competitiveness, Entrepreneur- ship, and Innovation, 2014–2020 (EPAnEK), under the call “Research-Create-Innovate”.

Funding

This research was funded by the Operational Programme Competitiveness, Entrepreneur- ship, and Innovation, 2014–2020 (EPAnEK), under the call “Research-Create-Innovate”.

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Authors and Affiliations

  1. Department of Agriculture, University of Ioannina, 45100, Ioannina, Arta, Greece

    Spiridon Mantzoukas

  2. School of Science and Technology, Hellenic Open University, Patra, Greece

    Spiridon Mantzoukas

  3. Laboratory of Applied Zoology and Parasitology, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Eleni I. Koutsogeorgiou

  4. Institute of Plant Breeding and Genetic Resources, Directorate General of Agricultural Research, Hellenic Agricultural Organization «Dimitra», PO Box 60458, 57001, Thermi, Greece

    Eleni I. Koutsogeorgiou & Stefanos S. Andreadis

  5. Institute of Industrial and Forage Crops, Department of Plant Protection, Directorate General of Agricultural Research, Hellenic Agricultural Organization «Dimitra», 26442, Patras, Greece

    Ioannis Lagogiannis

  6. Department of Agriculture, School of Geosciences, International Hellenic University, 57400, Sindos, Greece

    Nikoloz Gogolashvili, Georgios T. Fifis & Emmanuel I. Navrozidis

  7. Department of Nutritional Sciences & Dietetics, School of Health Sciences, International Hellenic University, 57400, Sindos, Greece

    Thomas Thomidis

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Contributions

This work was carried out in collaboration among all authors. SM, EIN, TT and SSA designed the study. EIK, NG and IL performed the laboratory experiments. SM, EIN, TT and SSA produced the manuscript. SM, EIN, TT and SSA revised the manuscript. SM, EIN, TT and SSA managed the literature searches. All authors contributed to revisions of the article. All authors read and approved the final manuscript.

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Correspondence to Spiridon Mantzoukas or Stefanos S. Andreadis.

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Mantzoukas, S., Koutsogeorgiou, E.I., Lagogiannis, I. et al. Effect of Entomopathogenic Fungi to Eggs and Nymphs Survival of Halyomorpha halys (Hemiptera: Pentatomidae) Under Laboratory Conditions. Curr Microbiol 81, 48 (2024). https://doi.org/10.1007/s00284-023-03547-y

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