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Variations in Rhizospheric and Endophytic Root Fungal Communities of Scrophularia ningpoensis in Different Producing Areas

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Abstract

Few studies have examined the association of factors associated with soil fertility and composition with the structure of microbial communities in the rhizosphere and endosphere. Hence, this study aimed to explore the effects of geographical differences on fungal communities in the roots of Scrophularia ningpoensis and the relationship between the fungal communities and secondary metabolic components in the host plant. We found that there was greater diversity in the fungal communities of the rhizosphere compartment than in endosphere communities. Ascomycota and Basidiomycota were dominant among the endosphere fungi, whereas Mortierellomycota was distributed in the rhizosphere. The composition of bulk soil obtained from different producing areas was significantly different, and the correlation between the rhizospheric and physicochemical compartments of the soil was higher than that observed with the endophytic compartment. Redundancy analysis and canonical correspondence analysis of the rhizospheric and endophytic samples revealed that the organic matter, total organic carbon, total nitrogen, and Hg levels were adequately correlated with the composition of rhizospheric and endophytic fungal communities. Multiple linear regression analyses facilitated the identification of potentially beneficial fungi whose abundance was correlated with levels of secondary metabolites, such as harpagide and harpagoside. These fungi could potentially provide valuable information regarding the use of S. ningpoensis in the medicinal plant industry.

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Acknowledgements

This study was supported by the Key Research and Development Program of Zhejiang Province of China (2021C04029), China; The Enshi Prefecture Science and technology program research and development project (D20210035), China; and Young Innovative Talents Project of Zhejiang Medical Health Science and Technology (2022RC052), China.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Zhejiang Chinese Medicial University, Hangzhou, 310053, China

    Dan Ren, Qingmei Sun, Bo Zhu & Luping Qin

  2. School of Biological Science and Technology, Hubei Minzu University, Enshi, 445000, China

    Kunyuan Guo

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  1. Dan Ren
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  2. Kunyuan Guo
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  3. Qingmei Sun
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  4. Bo Zhu
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Contributions

Dan Ren performed the experiments, analyzed the data, and wrote and revised the manuscript; Kunyuan Guo and Qing-mei Sun performed the experiments, and prepared figures and tables. Bo Zhu and Lu-ping Qin conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

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Correspondence to Bo Zhu or Luping Qin.

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Ren, D., Guo, K., Sun, Q. et al. Variations in Rhizospheric and Endophytic Root Fungal Communities of Scrophularia ningpoensis in Different Producing Areas. Curr Microbiol 80, 323 (2023). https://doi.org/10.1007/s00284-023-03439-1

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