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Isolation, Characterization, and Genomic Analysis of Multidrug-Resistant Rahnella aquatilis from Fruits in China

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Abstract

Many fruits are consumed raw and act as vehicles for spreading antibiotic-resistant bacteria to consumers; hence, preventing foodborne diseases and ensuring food safety of fresh fruits are challenging. In this study, we aimed to analyze contamination in fruits and characterize antibiotic resistance genes in pathogenic microorganisms isolated from fruits. Sixty fruit samples were collected and screened for pathogenic microorganisms. The strains were identified, and the minimum inhibitory concentration for various antibiotics was determined. Antibiotic-resistant strains were analyzed by whole-genome sequencing. We isolated strain L46 from lemon samples and identified it as Rahnella aquatilis using MALDI-TOF MS and 16S rRNA sequencing. The whole genome of R. aquatilis L46 was 4.94 Mb and contained 291 putative antibiotic resistance genes (6.53%), including the gene coding for β-lactamase RAHN-1 CTX-M-2 and conferring resistance to ampicillin, polymyxin B, nitrofurantoin, imipenem, aztreonam, and cefotaxime. Thus, fruits can pose a potential hazard to human health and require greater surveillance and attention, as they may contain pathogenic and multidrug-resistant bacteria.

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Data will be made available upon reasonable request.

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Acknowledgements

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Funding

This work was supported by the Medical and Health Science and Technology Project of Hangzhou Municipality (Grant no. A20200063) and the Hangzhou Agricultural and Society Development Project (Grant no. 202004A20).

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Authors and Affiliations

  1. Department of Infectious Disease Control and Prevention, Hangzhou Center of Disease Control and Prevention, Hangzhou, China

    Bing Wang, Hui Jin, Yongjie Xu & Zhou Sun

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  1. Bing Wang
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  2. Hui Jin
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  3. Yongjie Xu
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  4. Zhou Sun
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Contributions

BW: Conceptualization, sample collection, experimenting, data analysis, and writing. HJ: Reviewing and funding acquisition. YX: Data analysis. ZS: Funding acquisition.

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Correspondence to Bing Wang.

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The authors have no competing interests to declare that are relevant to the content of this article.

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This study did not require ethical approval as it did not involve animals and human material.

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Wang, B., Jin, H., Xu, Y. et al. Isolation, Characterization, and Genomic Analysis of Multidrug-Resistant Rahnella aquatilis from Fruits in China. Curr Microbiol 80, 321 (2023). https://doi.org/10.1007/s00284-023-03436-4

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  • DOI: https://doi.org/10.1007/s00284-023-03436-4

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