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Isolation and Characterization of Sphingomonas telluris, Sphingomonas caseinilyticus Isolated from Wet Land Soil

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Abstract

Two novel bacterial strains, designated as SM33T and NSE70-1T, were isolated from wet soil in South Korea. To get the taxonomic positions, the strains were characterized. The genomic information (both 16S rRNA gene and draft genome sequence analysis) show that both novel isolates (SM33T and NSE70-1T) belong to the genus Sphingomonas. SM33T share the highest 16s rRNA gene similarity (98.2%) with Sphingomonas sediminicola Dae20T. In addition, NSE70-1T show 96.4% 16s rRNA gene similarity with Sphingomonas flava THG-MM5T. The draft genome of strains SM33T and NSE70-1T consist of a circular chromosome of 3,033,485 and 2,778,408 base pairs with DNA G+C content of 63.9, and 62.5%, respectively. Strains SM33T and NSE70-1T possessed the ubiquinone Q-10 as the major quinone, and a fatty acid profile with C16:0, C18:1 2-OH, C16:1 ω7c/C16:1 ω6c (summed feature 3) and C18:1 ω7c/C18:1 ω6c (summed feature 8) as major fatty acids. The major polar lipids of SM33T and NSE70-1T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine, respectively. Moreover, genomic, physiological, and biochemical results allowed the phenotypic and genotypic differentiation of strains SM33T and NSE70-1T from their closest and other species of the genus Sphingomonas with validly published names. Therefore, the SM33T and NSE70-1T represent novel species of the genus Sphingomonas, for which the name Sphingomonas telluris sp. nov. (type strain SM33T = KACC 22222T = LMG 32193T), and Sphingomonas caseinilyticus (type strain NSE70-1T = KACC 22411T = LMG 32495T).

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Funding

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1I1A1A01061945), and by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Project No. 2019H1D3A1A02070958, and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Biotechnology, Hankyong National University, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan-Taek Im

  2. AceEMzyme Co., Ltd., Academic Industry Cooperation, Room 403, 327 Jungang-ro, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan-Taek Im

  3. HK Ginseng Research Center, 327 Jungang-no, Anseong-si, Gyeonggi-do, 17579, Republic of Korea

    Muhammad Zubair Siddiqi & Wan-Taek Im

  4. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat Sen University, Guangzhou, 510275, People’s Republic of China

    Govindan Rajivgandhi

  5. Department of Food Science and Technology, The University of Agriculture, Peshawar, 25130, Khyber Pakhtunkhwa, Pakistan

    Muhammad Faiq

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  1. Muhammad Zubair Siddiqi
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Contributions

Conceived and designed the experiments: MZ, GR, MF, WTI. Performed the experiments: MZS, WTI. Analyzed the data: MZS, WTI. Wrote the manuscript: MZS. Review: MF, WTI.

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Correspondence to Wan-Taek Im.

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The NCBI/EMBL/DDBJ accession numbers for the 16S rRNA and whole genome sequences of strains SM33T, and NSE70-1T are JAKZHW000000000, MW164938, and MZ675811, and JAMGBA000000000, respectively.

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Siddiqi, M.Z., Rajivgandhi, G., Faiq, M. et al. Isolation and Characterization of Sphingomonas telluris, Sphingomonas caseinilyticus Isolated from Wet Land Soil. Curr Microbiol 80, 264 (2023). https://doi.org/10.1007/s00284-023-03339-4

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