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Chitinolyticbacter albus sp. Nov., A Novel Chitin-Degrading Bacterium Isolated from Ancient Wood Rhizosphere Soil

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Abstract

In this study, a novel aerobic mesophilic bacterial strain with capable of degrading chitin, designated YIM B06366T, was isolated and classified. The rod-shaped, Gram-stain-negative, on-spore-forming bacterium originated from rhizosphere soil sample collected in Kunming City, Yunnan Province, southwest PR China. Strain YIM B06366T exhibited growth at temperatures between 20 and 35 °C (optimum, 30 °C) and at pH 6.0–8.0 (optimum, pH 6.0). The analysis of 16S rRNA gene sequence similarity revealed that strain YIM B06366T was most closely related to type strain Chitinolyticbacter meiyuanensis SYBC-H1T (98.9%). Phylogenetic analysis based on genome data indicated that strain YIM B06366T should be assigned to the genus Chitinolyticbacter. The Average Nucleotide Identity (ANI) and digital DNA-DNA Hybridization (dDDH) values between strain YIM B06366T and the reference strain Chitinolyticbacter meiyuanensis SYBC-H1T were 84.4% and 27.7%, respectively. The major fatty acids included Summed Feature 3 (C16:1 ω6c/C16:1 ω7c), Summed Feature 8 (C18:1 ω6c/C18:1 ω7c), and C16:0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipids, and two unidentified phospholipids. The predominant menaquinone was Q-8, and the genomic DNA G + C content was 64.1%. Considering the polyphasic taxonomic evidence, strain YIM B06366T is proposed as a novel species within the genus Chitinolyticbacter, named Chitinolyticbacter albus sp. nov. (type strain YIM B06366T = KCTC 92434T = CCTCC AB 2022163T).

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Fig. 1
Fig. 2

Abbreviations

TSA:

Tryptose soy agar

TSB:

Tryptic soy broth

ANI:

Average nucleotide identity

dDDH:

Digital DNA-DNA hybridization

DPG:

Diphosphatidylglycerol

PE:

Phosphatidylethanolamine

APL:

Aminophospholipids

PL1, PL2:

Unidentified phospholipids

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Acknowledgements

This work was supported by Major Science and Technology Projects of Yunnan Province (202202AE090015; 202002AA100007), Basic Research Project of Yunnan Province (202001BB050025) and the Top Young Talents of “ten thousand talents plan” in Yunnan Province, China, and the rural revitalization project of Serving Yunnan (CZ22624401).

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  1. Meng-Yu Zhang and Jiang-Yuan Zhao have equally contributed to this work.

Authors and Affiliations

  1. Key Laboratory for Microbial Resources of the Ministry of Education and School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Meng-Yu Zhang, Jiang-Yuan Zhao, Cheng Ling, Jing Tang, Song-Guo Liang, Jian-Yu Li, Xiao-Di Liu, Lu-Yao Feng, Zhang-Gui Ding, Ming-Gang Li & Shu-Kun Tang

  2. Department of Medicine, Qujing Vocational and Technical College, Qujing, 655000, People’s Republic of China

    Le-Le Li

  3. Institute of Agricultural Environmental Resources, Yunnan Academy of Agricultural Sciences, Kunming, 650205, People’s Republic of China

    Lu-Yao Feng, Pei-Wen Yang, Zhu-Feng Shi & Chui-Si Kong

  4. Yunnan Key Laboratory of Fermented Vegetables, Honghe, 661100, People’s Republic of China

    Jiang-Yuan Zhao, Ming-Gang Li & Shu-Kun Tang

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  1. Meng-Yu Zhang
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Contributions

MYZ, JYZ, and LLL, MGL, CSK and SKT designed the study, carried out the experiments and the data analysis, wrote and revised the manuscript. ZGD, CL, JT, SGL, JYL, PWY, XDL, LYF, and ZFS participated in the data analysis and revised the manuscript. All authors have read and approved the manuscript.

Corresponding authors

Correspondence to Le-Le Li, Chui-Si Kong or Shu-Kun Tang.

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Zhang, MY., Zhao, JY., Li, LL. et al. Chitinolyticbacter albus sp. Nov., A Novel Chitin-Degrading Bacterium Isolated from Ancient Wood Rhizosphere Soil. Curr Microbiol 80, 225 (2023). https://doi.org/10.1007/s00284-023-03333-w

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