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Influence of Light Color on Power Generation and Microalgae Growth in Photosynthetic Microbial Fuel Cell with Chlorella Vulgaris Microalgae as Bio-Cathode

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Abstract

Photosynthetic microbial fuel cell (PMFC) is an environmentally friendly sustainable technique for simultaneous wastewater treatment and power recovery. PMFC utilizes the microalgae to generate oxygen by photosynthesis process in the biocathode. Light sources and intensities have direct effect on chlorophyll pigment formation, photosynthesis processes and microalgae growth. In this study, Chlorella vulgaris was utilized as biocathode in PMFC fed with actual slaughterhouse wastewater. The biocathode was illuminated with florescent light as well as yellow, red and blue LED lights with light intensities of 67.46, 47.03, 26.18 and 4.70 µmol/m.s, respectively. Power output and microalgae growth were considered in evaluating the PMFC performance. Results demonstrated that the highest power output was 217.04 mW/m2 generated under florescent light compared to 28.41, 171.08, and 21.65 mW/m2 observed under yellow, red and blue LEDs, respectively. Additionally, statistical analysis was performed using fifth-degree polynomial model which fitted well the experimental data with a determination coefficient (R2) > 0.97. The results reflected a high confidence level in depicting the growth mechanism of Chlorella vulgaris under lighting sources with different light colors and intensities.

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References

  1. Denis GS, Parker P (2009) Community energy planning in Canada: the role of renewable energy. Renew Sust Energ Rev 13:2088–2095. https://doi.org/10.1016/j.rser.2008.09.030

    Article  Google Scholar 

  2. Ismail ZZ, Habeeb AA (2015) Pharmaceutical wastewater treatment associated with renewable energy generation in microbial fuel cell based on mobilized electroactive biofilm on zeolite bearer. J Eng 21:35–44

    Google Scholar 

  3. Ragauskas AJ, Williams CK, Davison B, Britovsek G, Cairney J, Eckert CA, Frederick WJ, Hallett JP, Leak DJ, Liotta CL, Mielenz JR, Murphy R, Temple R, Tschaplinski T (2006) The path forward for biofuels and biomaterials. Science 311:484–489. https://doi.org/10.1126/science.1114736

    Article  CAS  PubMed  Google Scholar 

  4. Logan BE, Hamelers HWM, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey R (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192. https://doi.org/10.1021/es0605016

    Article  CAS  PubMed  Google Scholar 

  5. Rosenbaum M, Zhen M, He Z, Angenent TL (2010) Light energy to bioelectricity: photosynthetic microbial fuel cells. Curr Opin Biotechnol 21:259–264. https://doi.org/10.1016/j.copbio.2010.03.010

    Article  CAS  PubMed  Google Scholar 

  6. Hall DO, Markov SA, Watanabe Y, Rao KK (1995) The potential application of cyanobacterial photosynthesis for clean technologies. Photosynth Res 46:159–167. https://doi.org/10.1007/BF00020426

    Article  CAS  PubMed  Google Scholar 

  7. Strik DPBTB, Timmers RA, Helder M, Steinbusch KJJ, Hamelers HVM, Buisman CJ (2011) Microbial solar cells: applying photosynthetic and electrochemically active organisms. Trends Biotechnol 1:41–49. https://doi.org/10.1016/j.tibtech.2010.10.001

    Article  CAS  Google Scholar 

  8. Pirt JS (1986) The thermodynamic efficiency (quantum demand) and dynamics of photosynthetic growth. New Phytol 102:3–37. https://doi.org/10.1111/j.1469-8137.1986.tb00794

    Article  Google Scholar 

  9. Xing D, Cheng S, Regan JM, Logan BE (2009) Microbial communities in acetate- and glucose-fed microbial fuel cells in the presence of light. Biosens Bioelectron 25:105–111. https://doi.org/10.1016/j.bios.2009.06.013

    Article  CAS  PubMed  Google Scholar 

  10. Das P, Lei W, Aziz SS, Obbard JP (2010) Enhanced algae growth in both phototrophic and mixotrophic culture under blue light. Bioresour Technol 102:3883–3887. https://doi.org/10.1016/j.biortech.2010.11.102

    Article  CAS  PubMed  Google Scholar 

  11. Zheng W, Cai T, Huang M, Chen D (2017) Comparison of electrochemical performances and microbial community structures of two photosynthetic microbial fuel cells. J Biosci Bioeng 124:551–558. https://doi.org/10.1016/j.jbiosc.2017.05.013

    Article  CAS  PubMed  Google Scholar 

  12. Wang C-T, Huang Y-S, Sangeetha T, Chen Y-M, Chong W-T, Ong H-C, Zhao F, Yan W-M (2018) Novel bufferless photosynthetic microbial fuel cell (PMFCs) for enhanced electrochemical performance. Bioresour Technol 255:83–87. https://doi.org/10.1016/j.biortech.2018.01.086

    Article  CAS  PubMed  Google Scholar 

  13. Pei H, Yang Z, Nie C, Hou Q, Zhang L, Wang Y, Zhang S (2018) Using a tubular photosynthetic microbial fuel cell to treat anaerobically digested effluent from kitchen waste: mechanisms of organics and ammonium removal. Bioresour Technol 256:11–16. https://doi.org/10.1016/j.biortech.2018.01.144

    Article  CAS  PubMed  Google Scholar 

  14. Olias LG, Cameron PJ, Lorenzo MD (2019) Effect of electrode properties on the performance of a photosynthetic microbial fuel cell for atrazine detection. Front Energy Res 7:1–11. https://doi.org/10.3389/fenrg.2019.00105

    Article  Google Scholar 

  15. Lan JCW, Kumaran R, Chun-Mao H, Chung-Ming C (2013) The impact of monochromatic blue and red LED light upon performance of photo microbial fuel cells (PMFCs) using Chlamydomonas reinhardtii transformation F5 as biocatalyst. Biochem Eng J 78:39–43. https://doi.org/10.1016/j.bej.2013.02.007

    Article  CAS  Google Scholar 

  16. Bazdar E, Roshandel R, Yaghmaei S, Mardanpour MM (2018) The effect of different light intensities and light/dark regimes on the performance of photosynthetic microalgae microbial fuel cell. Bioresour Technol 261:350–360. https://doi.org/10.1016/j.biortech.2018.04.026

    Article  CAS  PubMed  Google Scholar 

  17. Satthong S, Saego K, Kitrungloadjanaporn P, Nuttavut N, Amornsamankul S, Triampo W (2019) Modeling the effects of light sources on the growth of algae. Adv Differ Equ 170:1–6. https://doi.org/10.1186/s13662-019-2112-6

    Article  Google Scholar 

  18. Ismail ZZ, Mohammed AJ (2017) Biotreatment of slaughterhouse wastewater accompanied with electricity generation and nutrients recovery in microbial fuel cell. J Eng 23:94–105

    Google Scholar 

  19. Fadhil, S.H., and Z.Z. Ismail (2021) Bioremediation of real-field slaughterhouse wastewater associated with power generation in algae-photosynthetic microbial fuel cell. Bioremediat J https://doi.org/10.1080/10889868.2021.1988507.

  20. Olivera RH, Dueñas-Gonza A, Yapo-Pari U, Vega P, Romero-Ugarte M, Tapia J, Molina L, Lazarte-Rivera A, Pacheco-Salazar DG, Esparza M (2018) Bioelectrogenesis with microbial fuel cells (MFCs) using the microalga Chlorella vulgaris and bacterial communities. Electron J Biotechnol 31:34–43

    Article  Google Scholar 

  21. Ramaraj R, David D-W, Tsai D, D W, Chen PH (2013) Chlorophyll is not accurate measurement for algal biomass. Chiang Mai J Sci 40:1–9

    Google Scholar 

  22. Ritchie RJ (2006) Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth Res 89:27–41. https://doi.org/10.1007/s11120-006-9065-9

    Article  CAS  PubMed  Google Scholar 

  23. Kannan N, Donnellan P (2021) Algae-assisted microbial fuel cells: a practical overview. Bioresour Technol Rep. https://doi.org/10.1016/j.biteb.2021.100747

    Article  Google Scholar 

  24. Panahi Y, Khosroshahi AY, Sahebkar A, Heidari HR (2019) Impact of cultivation condition and media content on Chlorella vulgaris composition. Adv Pharm Bull 9:182–194. https://doi.org/10.15171/apb.2019.022

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  25. Lv B, Liu Z, Chen Y, Lan S, Mao J, Gu Z, Wang A, Yu F, Zheng X, Vasquez HE (2022) Effect of different colored LED lighting on the growth and pigment content of Isochrysis zhanjiangensis under laboratory conditions. J Mar Sci Eng 10:1752. https://doi.org/10.3390/jmse10111752

    Article  Google Scholar 

  26. Tsai, C.-C., Huang, C.-F., Chen, C.-F., and C.-F. Yue (2017) The study of LED light source illumination conditions for ideal algae cultivation. Proceeding of SPIE 10107, Smart Photonic and Optoelectronic Integrated Circuits XIX, https://doi.org/10.1117/12.2255625

  27. Saba B, Christy AD, Zhongtang Y, Co AC (2017) Sustainable power generation from bacterio-algal microbial fuel cells (MFCs): an overview. Renew Sust Energ Rev 73:75–84. https://doi.org/10.1016/j.rser.2017.01.115

    Article  CAS  Google Scholar 

  28. Zhang Y, Zhao Y, Zhou M (2019) A photosynthetic algal microbial fuel cell for treating swine wastewater. Environ Sci Pollut Res 26:6182–6190. https://doi.org/10.1007/s11356-018-3960-4

    Article  CAS  Google Scholar 

  29. Takahashi S, Badger MR (2011) Photo protection in plants: a new light on photosystem II damage. Trends Plant Sci 16:53–60. https://doi.org/10.1016/j.tplants.2010.10.001

    Article  CAS  PubMed  Google Scholar 

  30. Oren A (2007) Diversity of organic osmotic compounds and osmotic adaptation in cyanobacteria and algae. Springer, Netherland. https://doi.org/10.1007/978-1-4020-6112-7_35

    Article  Google Scholar 

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Acknowledgements

The authors are grateful to the staff of Al-Rustamia Treatment Plant and Al-Shoula Slaughterhouse Facility in Baghdad, Iraq for their technical support and the continuous supply of fresh samples.

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  1. Department of Environmental Engineering, University of Baghdad, Baghdad, Iraq

    Safa H. Fadhil & Zainab Z. Ismail

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  1. Safa H. Fadhil
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  2. Zainab Z. Ismail
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SHF contributed to experiment performing, result presenting, and manuscript writing. ZZI contributed to result interpretation, data processing and analysis, and manuscript writing, overall organization of the study, and approved the final version for submission.

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Correspondence to Zainab Z. Ismail.

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Fadhil, S.H., Ismail, Z.Z. Influence of Light Color on Power Generation and Microalgae Growth in Photosynthetic Microbial Fuel Cell with Chlorella Vulgaris Microalgae as Bio-Cathode. Curr Microbiol 80, 177 (2023). https://doi.org/10.1007/s00284-023-03292-2

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