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Euphorbia helioscopia a Putative Plant Reservoir of Pathogenic Curtobacterium flaccumfaciens

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Abstract

The endophyte EHF3 strain was isolated in Algeria from an Euphorbia helioscopia plant growing in a fallow field. The strain was characterized by biochemical and physiological tests and assayed for the production of secondary metabolites involved in biocontrol, for plant growth promotion ability and for pathogenicity. The strain was identified by BIOLOG test as Curtobacterium flaccumfaciens. Biochemical and physiological characterization revealed that the strain was able to secrete protease, caseinase and amylase enzymes, to grow up to 37 °C and at pH values 5 to 9. C. flaccumfaciens EHF3 strain was incapable of solubilizing phosphorus and to produce IAA, HCN siderophores and phenazine compounds. The strain showed a moderate swimming and swarming motility and produced biofilm. EHF3 strain was positive at the hypersensitivity test on tobacco plants and induced symptoms on three varieties of bean resembling to those of the bacterial wilt disease induced by C. flaccumfaciens pv. flaccumfaciens. The preliminary data reported in this study, regarding the detection of a pathogenic C. flaccumfaciens strain, as endophyte of a E. helioscopia plant, highlight the role of non-host plants as reservoir of this bacterial pathogen.

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The authors did not receive support from any organization for the submitted work.

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Authors and Affiliations

  1. Faculté des Sciences de la Nature et de la Vie—University of Blida 1, 09000, Blida, Algeria

    Zoulikha Krimi, Chahrazed Ziouche, Lamia Tafifet, Hafidha Djellout & Fadhéla Mohamed-Mahmoud

  2. Institute for Sustainable Plant Protection—CNR, Via Madonna del Piano, 10, 50019, Sesto Fiorentino, FI, Italy

    Aida Raio

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  1. Zoulikha Krimi
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  2. Chahrazed Ziouche
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  3. Lamia Tafifet
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  4. Hafidha Djellout
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  5. Fadhéla Mohamed-Mahmoud
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  6. Aida Raio
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Contributions

Conceptualization, ZK and AR; methodology ZK and AR; formal analysis, CZ; sample preparation, CZ, LT, HD and FMM; data analysis, writing, original draft preparation, review and editing, supervision, ZK and AR. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Aida Raio.

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Supplementary file1 (DOCX 26 KB)

284_2023_3271_MOESM2_ESM.tif

Supplementary file2 Fig. S1 Phylogenetic tree showing relatedness between C. flaccumfaciens EHF3, Curtobacterium spp. and Rathayibacter tritici as obtained by Neighbor-Joining analysis [15] (TIF 16 KB)

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Krimi, Z., Ziouche, C., Tafifet, L. et al. Euphorbia helioscopia a Putative Plant Reservoir of Pathogenic Curtobacterium flaccumfaciens. Curr Microbiol 80, 154 (2023). https://doi.org/10.1007/s00284-023-03271-7

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