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Insights into Regulating Mechanism of Mutagenesis Strains of Elizabethkingia meningoseptica sp. F2 by Omics Analysis

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Abstract

Vitamin K2 plays an important role in electron transport, blood coagulation, and calcium homeostasis, and researchers have been trying to use microbes to produce it. Although our previous studies have shown that gradient radiation, breeding, and culture acclimation can improve vitamin K2 production in Elizabethkingia meningoseptica, the mechanism is still unclear. This study is the first which performs genome sequencing of E. meningoseptica sp. F2 as a basis for subsequent experiments and further comparative analyses with other strains. Comparative metabolic pathway analysis of E. meningoseptica sp. F2, E. coli, Bacillus subtilis, and other vitamin K2 product strains revealed that the mevalonate pathway of E. meningoseptica sp. F2 is different in bacteria at the system level. The expressions of menA, menD, menH, and menI in the menaquinone pathway and idi, hmgR, and ggpps in the mevalonate pathway were higher than those in the original strain. A total of 67 differentially expressed proteins involved in the oxidative phosphorylation metabolic pathway and citric acid cycle (TCA cycle) were identified. Our results reveal that combined gradient radiation breeding and culture acclimation can promote vitamin K2 accumulation probably by regulating the vitamin K2 pathway, oxidative phosphorylation metabolism pathway, and the citrate cycle (TCA cycle).

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Funding

This work was sponsored by financial support from National Natural Science Foundation of China (32070088), the China National Key Research and Development Programme (2019YFA0904300, 2019YFA0904304), Key Research and Development Plan of Anhui Province (1804b06020342), Major Projects of Science and Technology of Anhui Province (202103a06020003), and Natural Science Foundation of Anhui Province (1908085MB48 and 1908085MB43).

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Authors and Affiliations

  1. Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Qiang Yang, Zhiming Zheng, Peng Wang, Li Wang, Han Wang, Mengxue Zhang & Genhai Zhao

  2. University of Science and Technology of China, Hefei, 230026, People’s Republic of China

    Qiang Yang, Han Wang & Mengxue Zhang

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  1. Qiang Yang
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Contributions

ZZM and ZGH designed this study. YQ performed the research, analyzed the data, and wrote the paper; WP and WL participated in data analysis, figure preparation, and manuscript writing. WH and ZMX revised the manuscript.

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Correspondence to Zhiming Zheng or Genhai Zhao.

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Yang, Q., Zheng, Z., Wang, P. et al. Insights into Regulating Mechanism of Mutagenesis Strains of Elizabethkingia meningoseptica sp. F2 by Omics Analysis. Curr Microbiol 80, 183 (2023). https://doi.org/10.1007/s00284-023-03270-8

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