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Mutualistic Effect of Macronutrients Availing Microbes on the Plant Growth Promotion of Finger Millet (Eleusine coracana L.)

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Abstract

Globally, man-made agrochemicals plays crucial role in plant growth promotion and boost crop yield. The agrochemicals overuse leaves the detrimental damage on the environment and humans. Biostimulants developed from single or multiple microbes (archaea, bacteria, and fungi) could be the appropriate alternative of agrochemical which sustains the agriculture as well as environment. In the present investigation, 93 beneficial bacteria associated with rhizospheric and endophytic region were isolated using diverse growth media. The isolated bacteria were screened for macronutrients availing traits including dinitrogen fixation, phosphorus and potassium solubilization. The bacterial consortium was developed using selected bacteria with multifunctional attributes and evaluated for the growth promotion of finger millet crop. Three potent NPK strains were identified as Erwinia rhapontici EU-FMEN-9 (N-fixer), Paenibacillus tylopili EU-FMRP-14 (P-solubilizer) and Serratia marcescens EU-FMRK-41 (K-solubilizer) using 16S rRNA gene sequencing and BLAST analysis. The developed bacterial consortium inoculation on finger millet resulted in the improvement of growth and physiological parameters with respect to chemical fertilizer and control. The compatible mixture of bacteria was found to have more ability to increase the growth of finger millet and it might be utilized as biostimulants for nutri-cereal crops growing in hilly regions.

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Acknowledgements

The authors are grateful to the Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib and Department of Environment, Science & Technology (DEST), Shimla, HP funded project “Development of microbial consortium as bio-inoculants for drought and low temperature growing crops for organic farming in Himachal Pradesh” for providing the facilities support, to undertake the investigations.

Funding

The present study was funded by Department of Biotechnology, Akal College of Agriculture, Eternal University, Baru Sahib and Department of Environment, Science & Technology (DEST), Shimla, HP funded project “Development of microbial consortium as bio-inoculants for drought and low temperature growing crops for organic farming in Himachal Pradesh”.

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Authors and Affiliations

  1. Department of Biotechnology, Microbial Biotechnology Laboratory, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India

    Tanvir Kaur, Rubee Devi, Rajeshwari Negi & Ajar Nath Yadav

  2. Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India

    Divjot Kour

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  1. Tanvir Kaur
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Contributions

Tanvir Kaur compile the manuscript; Rubee Devi and Rajeshwari Negi helps in data compilation; Divjot Kour read and review the manuscript and Ajar Nath Yadav hypothesized the manuscript.

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Correspondence to Ajar Nath Yadav.

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Kaur, T., Devi, R., Negi, R. et al. Mutualistic Effect of Macronutrients Availing Microbes on the Plant Growth Promotion of Finger Millet (Eleusine coracana L.). Curr Microbiol 80, 186 (2023). https://doi.org/10.1007/s00284-023-03255-7

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