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Salinibacterium sedimenticola sp. nov., Isolated from Tidal Flat Sediment

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Abstract

An actinobacterium, designated as SYSU T00001T, was isolated from a tidal flat sediment sample from Guangdong province, China. Cells were Gram-stain-positive, aerobic, motile and short rod-shaped. Colonies on marine agar 2216 were smooth, yellow-pigmented, and circular with low convexity. The isolate was able to grow at the temperature range 4–37 °C (optimum 30 °C), at pH 4.0–10.0 (optimum 7.0) and in the presence of 0–10% (w/v) NaCl. The major menaquinones were MK-11 and MK-10. The cell wall contained alanine, glutamic acid, lysine and ornithine. The major fatty acids were C19:0 cyclo ω8c (35.7%) and anteiso C15:0 (26.0%). The polar lipids consisted of one diphosphatidyl glycerol, one unidentified glycolipid and one unknown lipid. Whole genome sequencing of strain SYSU T00001T revealed 2,837,702 bp with a DNA G + C content of 67.8%. Phylogenetic analyses clearly demonstrated that strain SYSU T00001T belonged to the genus Salinibacterium, and the highest 16S rRNA gene similarity to Salinibacterium hongtaonis 194T (97.8%). The ANI and dDDH values of strain SYSU T00001T relative to Salinibacterium hongtaonis 194T were 74.5% and 19.5%, respectively. According to our data, strain SYSU T00001T represents a novel species of the genus Salinibacterium, for which the name Salinibacterium sedimenticola sp. nov. is proposed, the type strain is SYSU T00001T (= GDMCC 1.3283T = KCTC 49758T).

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Data Availability

The 16S rRNA gene sequence and draft genome of strain SYSU T00001T have been deposited in GenBank/EMBL/DDBJ.

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Acknowledgements

The authors are grateful to Professor Yu-Guang Zhou (CGMCC, China) for providing the reference type strain.

Funding

This research was financially supported by National Science and Technology Fundamental Resources Investigation Program of China (Grant No. 2019FY100700), National Natural Science Foundation of China (Nos: 32270076 and 32000005) and the Key-Area Research and Development Program of Guangdong Province (No. 2022B0202110001). WJ Li was also supported by Introduction project of high-level talents in Xinjiang Uygur Autonomous Region.

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  1. Chun-Yan Lu and Lei Dong contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Chun-Yan Lu, Lei Dong, Shuai Li, Wen-Hui Lian, Zhi-Liang Lin, Zhuo-Huan Zheng & Wen-Jun Li

  2. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Lei Gao, Bao-Zhu Fang & Wen-Jun Li

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Contributions

BZF and WJL jointly conceived the study. Authors CYL, LD, SL, WHL, ZLL, ZHZ and LG performed sampling, isolation and identification. CYL, LD and SL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bao-Zhu Fang or Wen-Jun Li.

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Lu, CY., Dong, L., Li, S. et al. Salinibacterium sedimenticola sp. nov., Isolated from Tidal Flat Sediment. Curr Microbiol 80, 142 (2023). https://doi.org/10.1007/s00284-023-03243-x

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