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The Antibacterial Effect of Ciprofloxacin Loaded Calcium Carbonate (CaCO3) Nanoparticles Against the Common Bacterial Agents of Osteomyelitis

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Abstract

The present study aimed to investigate the biocompatibility, antibacterial/anti-biofilm effects of ciprofloxacin-loaded calcium carbonate (Cip- loaded CaCO3) nanoparticles against the common organisms responsible for osteomyelitis. The antibacterial and biofilm inhibitory activities were studied by determination of minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentrations (MBICs), respectively. Hemolytic effects were determined for studying hemocompatibility. The SDS-PAGE method was used to study the interaction of Cip- loaded CaCO3 with plasma proteins. The effects of Cip- loaded CaCO3 on the cell viability of human bone marrow mesenchymal stem cells (hBM-MSCs) was detected. The Cip- loaded CaCO3 nanoparticles were shown a significant antimicrobial effect at lower concentrations than free ciprofloxacin. No significant hemolytic effect was observed. The Cip- loaded CaCO3 nanoparticles have shown interaction with apolipoprotein A1 (28 kDa) and albumin (66.5 kDa). The viability of hBM-MSCs treated with Cip- loaded CaCO3 was more than 96%. Our results indicated that Cip-loaded CaCO3 nanoparticles had favorable in vitro compatibility with human red blood cells, antimicrobial effects, and low cytotoxicity.

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Acknowledgements

This study was supported by Elite Researcher Grant Committee under award number (No. 982973) from the National Institutes for Medical Research Development (NIMAD), Tehran, Iran.

Funding

This study was supported by Elite Researcher Grant Committee under award number (No. 982973) from the National Institutes for Medical Research Development (NIMAD), Tehran, Iran.

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Authors and Affiliations

  1. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Yousef Memar & Mohammad Ahangarzadeh Rezaee

  2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Yousef Memar

  3. Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Ahangarzadeh Rezaee

  4. Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Barzegar-Jalali

  5. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Tooba Gholikhani

  6. Nanora Pharmaceuticals Ltd., Tabriz, Iran

    Tooba Gholikhani

  7. Research Center for Pharmaceutical Nanotechnology, and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Khosro Adibkia

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  1. Mohammad Yousef Memar
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Correspondence to Khosro Adibkia.

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Memar, M.Y., Ahangarzadeh Rezaee, M., Barzegar-Jalali, M. et al. The Antibacterial Effect of Ciprofloxacin Loaded Calcium Carbonate (CaCO3) Nanoparticles Against the Common Bacterial Agents of Osteomyelitis. Curr Microbiol 80, 173 (2023). https://doi.org/10.1007/s00284-023-03234-y

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