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The Complete Genome Sequence of a Gossypol-Degrading Bacterial Strain, Raoultella sp. YL01

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Abstract

Cottonseed meal is an important source of plant protein for the meal fodder materials. But its usage in animal breeding industry is limited by a type of toxic phenol, gossypol, that has toxic effects on animal health. Microbial degradation is a promising way to lower down gossypol in cottonseed meal. However, the molecular mechanisms of bio-degradation of gossypol is still unclear. In this study we isolated a gossypol-degrading bacterial strain, YL01, and sequenced its complete genome via Oxford Nanopore sequencing method. There is a chromosome (5,737,005 bp) and a plasmid (136,446 bp) in YL01. 5489 protein coding genes in total were functionally annotated. 16S rRNA analysis showed that YL01 taxonomically belongs to the genus of Raoultella. YL01 is the first published complete genome sequence of microbes capable of gossypol degradation. Gene function annotation showed that 126 protein coding genes may involve in gossypol catabolism. Sequence similarity analysis showed that, as the only gossypol-degrading strain in the genus of Raoultella, YL01 uniquely holds 260 genes that are not possessed by other Raoultella strains. Our work gives a preliminary list for genes responsible for gossypol degradation but further investigations are needed to completely disclose this molecular processes.

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Data Availability

YL01 chromosome and plasmid sequences are available from CNGB Sequence Archive (CNSA) of China National GeneBank DataBase (CNGBdb) with accession number CNA0050668 and CNA0050669, respectively.

Code Availability

Customized R codes and Linux shell codes are available upon request.

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Acknowledgements

The authors would like to thank Miss Junying Lv for her help and assistance in soil sample collection.

Funding

This work was financially supported by the National Natural Science Foundation of China under Grant No. 32070098.

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Author notes

  1. Si Chen and Weishu Zhang have contributed equally to this work.

Authors and Affiliations

  1. School of of Life Sciences and Technology, Wuhan Polytechnic University, Wuhan, 430023, People’s Republic of China

    Si Chen, Weishu Zhang, Huiling Zhang, Ying Cui, Feng Wang, Jing Wu, Hongjun Chao & Dazhong Yan

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  1. Si Chen
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Contributions

SC WZ and HZ wrote the manuscript. SC, YC and JW analyzed the data. WZ and FW performed bacterial strain isolation. HJC and DY designed the experiment.

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Correspondence to Hongjun Chao or Dazhong Yan.

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Chen, S., Zhang, W., Zhang, H. et al. The Complete Genome Sequence of a Gossypol-Degrading Bacterial Strain, Raoultella sp. YL01. Curr Microbiol 80, 163 (2023). https://doi.org/10.1007/s00284-023-03204-4

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