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Effects of Green Manures on Rhizosphere Fungal Community Composition of Cucumber Seedlings

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Abstract

Green manures are usually used to improve soil health and increase crop productivity. The activity and composition of the soil microbial community could be altered by green manures. High-throughput amplicon sequencing was used to assess the effects of green manures of wheat (Triticum aestivum) and Indian mustard (Brassica juncea) on the rhizosphere fungal community composition of cucumber (Cucumis sativus L). Results showed that green manures of wheat and Indian mustard altered the composition but not the diversity of rhizosphere fungal communities of cucumber Contents of inorganic N, Olsen P, and available K in bulk soil decreased by green manure treatments. Ascomycota, Zygomycota, and Basidiomycota were the predominant phyla in soil, and their relative abundance changed after treatment with wheat and Indian mustard. The relative abundance of Basidiomycota was increased in the green manure of wheat, while that of Zygomycota was decreased in the green manure of Indian mustard. The relative abundance of Ascomycota increased in both wheat and Indian mustard. Green manures of wheat and Indian mustard also increased the relative abundances of unclassified Sordariomycetes and Fusarium spp., whereas they decreased the relative abundances of Pseudallescheria, Mortierella, Kernia, and unclassified Chaetomiaceae spp. Compared with other treatments, green manures of wheat increased the relative abundance of Waitea sp. and decreased the relative abundance of Cephaliophora sp. Indian mustard increased the relative abundance of Humicola sp.

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References

  1. Jin X, Wu FZ, Zhou XG (2020) Different toxic effects of ferulic and p-hydroxybenzoic acids on cucumber seedling growth were related to their different influences on rhizosphere microbial composition. Biol Fert Soils 56:125–136

    Article  CAS  Google Scholar 

  2. Li T, Ullah S, Liang H, Ali I, Jiang L (2020) The enhancement of soil fertility, dry matter transport and accumulation, nitrogen uptake and yield in rice via green manuring. Phyton 1(20):65

    Google Scholar 

  3. Teófilo TMD, Mendes KF, Fernandes BCC, Oliveira FS, Silva DV et al (2020) Phytoextraction of diuron, hexazinone, and sulfometuron-methyl from the soil by green manure species. Chemosphere 256:127059

    Article  PubMed  Google Scholar 

  4. Timofeeva Y, Purtova L, Emelyanov A, Burdukovskii M, Kiseleva I, Sidorenko M (2020) Contents, distribution, and fractionation of soil organic carbon and trace elements in soils under a green manure application. Soil Water Res 16(1):50–58

    Article  Google Scholar 

  5. Jin X, Zhang JH, Shi YJ, Wu FZ, Zhou XG (2019) Green manures of Indian mustard and wild rocket enhance cucumber resistance to Fusarium wilt through modulating rhizosphere bacterial community composition. Plant Soil 441:283–300

    Article  CAS  Google Scholar 

  6. Toda M, Uchida Y (2017) Long-term use of green manure legume and chemical fertiliser affect soil bacterial community structures but not the rate of soil nitrate decrease when excess carbon and nitrogen are applied. Soil Res 55(6):524–533

    Article  Google Scholar 

  7. Exposito RG, Bruijn I, Postma J, Raaijmakers JM (2017) Current insights into the role of rhizosphere bacteria in disease suppressive soils. Front Microbiol. 8:2529

    Article  Google Scholar 

  8. Tran HT, Lin CT, Bui XT, Dang BT, Cheruiyot NK, Hoang HG, Itayama T, Vu CT (2020) Bacterial community progression during food waste composting containing high dioctyl terephthalate (DOTP) concentration. Chemosphere 265:129064

    Article  PubMed  Google Scholar 

  9. Nair A, Ngouajio M (2012) Soil microbial biomass, functional microbial diversity, and nematode community structure as affected by cover crops and compost in an organic vegetable production system. Appl Soil Ecol 58:45–55

    Article  Google Scholar 

  10. Siciliano SD, Palmer AS, Winsley T, Lamb E, Bissett A et al (2014) Soil fertility is associated with fungal and bacterial richness, whereas pH is associated with community composition in polar soil microbial communities. Soil Biol Biochem 78:10–20

    Article  CAS  Google Scholar 

  11. Wal A, Geydan TD, Kuyper TW, Boer W (2013) A thready affair: linking fungal diversity and community dynamics to terrestrial decomposition processes. FEMS Microbiol Rev 37(4):477–494

    Article  PubMed  Google Scholar 

  12. Jin X, Wang ZL, Wu FZ, Li XG, Zhou XG (2022) Litter mixing alters microbial decomposer community to accelerate tomato root litter decomposition. Microbiol Spectr 10:e00186-e222

    Article  PubMed  PubMed Central  Google Scholar 

  13. Zhou XG, Liu J, Wu FZ (2017) Soil microbial communities in cucumber monoculture and rotation systems and their feedback effects on cucumber seedling growth. Plant Soil 415:507–520

    Article  CAS  Google Scholar 

  14. Jin X, Bai Y, Rahman MKU, Kang X, Pan K, Wu F, Pommier T, Zhou X, Wei Z (2022) Biochar stimulates tomato roots to recruit a bacterial assemblage contributing to disease resistance against Fusarium wilt. iMeta 1(3):e37

    Article  Google Scholar 

  15. Zhou XG, Zhang XH, Ma CL, Wu FZ, Jin X, Dini-Andreote F, Wei Z (2022) Biochar amendment reduces cadmium uptake by stimulating cadmium-resistant PGPR in tomato rhizosphere. Chemosphere 307:136138

    Article  CAS  PubMed  Google Scholar 

  16. Longa CMO, Nicola L, Antonielli L, Mescalchin E, Zanzotti R, Turco E, Pertot I (2017) Soil microbiota respond to green manure in organic vineyards. J Appl Microbiol 123(6):1547–1560

    Article  CAS  PubMed  Google Scholar 

  17. Martinez-Diz MD, Andrés-Sodupe M, Bujanda R, Díaz-Losada E, Eichmeier A, Gramaje D (2019) Soil-plant compartments affect fungal microbiome diversity and composition in grapevine. Fungal Ecol 41:234–244

    Article  Google Scholar 

  18. Riga E (2011) The effects of Brassica green manures on plant parasitic and free living nematodes used in combination with reduced rates of synthetic nematicides. J Nematol 43(2):119–121

    PubMed  PubMed Central  Google Scholar 

  19. Oueslati O (2003) Allelopathy in two durum wheat (Triticum durum L.) varieties. Agr Ecosyst Environ 96(1–3):161–163

    Article  Google Scholar 

  20. Boer W, Folman LB, Summerbell RC, Boddy L (2005) Living in a fungal world: impact of fungi on soil bacterial niche development. Fems Microbiol Rev 29(4):795–811

    Article  PubMed  Google Scholar 

  21. Zhou XG, Zhang JF, Pan DD, Ge X, Jin X, Chen S, Wu FZ (2018) p-Coumaric can alter the composition of cucumber rhizosphere microbial communities and induce negative plant-microbial interactions. Biol Fert Soils 54(3):363–372

    Article  CAS  Google Scholar 

  22. Unterseher M, Persoh D, Schnittler M (2013) Leaf-inhabiting endophytic fungi of European Beech (Fagus sylvatica L.) co-occur in leaf litter but are rare on decaying wood of the same host. Fungal Divers 60(1):43–54

    Article  Google Scholar 

  23. Helgason T, Fitter AH (2009) Natural selection and the evolutionary ecology of the arbuscular mycorrhizal fungi (Phylum Glomeromycota). J Exp Bot 9(60):2465–2480

    Article  Google Scholar 

  24. Kepler RM, Maul JE, Rehner SA (2017) Managing the plant microbiome for biocontrol fungi: examples from Hypocreales. Curr Opin Microbiol 37:48–53

    Article  CAS  PubMed  Google Scholar 

  25. Carvalho JCB, Sousa KCI, Brito DC, Chaibub AA, Luzini AP, Cortes MVCB, Filippi MCC, Kato L, Vaz BG, Costa HB, Romao W, Araujo LG (2015) Biocontrol potential of Waitea circinata, an orchid mycorrhizal fungus, against the rice blast fungus. Trop Plant Pathol 40(3):151–159

    Article  Google Scholar 

  26. Xiong W, Guo S, Jousset A, Zhao QY, Wu HS, Li R, Kowalchuk GA, Shen QR (2017) Bio-fertilizer application induces soil suppressiveness against Fusarium wilt disease by reshaping the soil microbiome. Soil Biol Biochem 114:238–247

    Article  CAS  Google Scholar 

  27. Li F, Chen L, Redmile-Gordon M, Zhang JB, Zhang CZ, Ning Q, Li W (2018) Mortierella Elongata’s roles in organic agriculture and crop growth promotion in a mineral soil. Land Degrad Dev 29(6):1642–1651

    Article  Google Scholar 

  28. Breitkreuz C, Heintz-Buschart A, Buscot F, Wahdan SFM, Tarkka M, Reitz T (2021) Can we estimate functionality of soil microbial communities from structure-derived predictions? A reality test in agricultural soils. Microbiol Spectr 9(1):e00278

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Jin X, Rahman MKU, Ma C, Zheng X, Wu F, Zhou X (2023) Silicon modification improves biochar’s ability to mitigate cadmium toxicity in tomato by enhancing root colonization of plant-beneficial bacteria. Ecotoxicol Environ Saf 249:114407

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (32072655, 32272792) and China Agriculture Research System of MOF and MARA (CARS-23-B-10).

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Authors and Affiliations

  1. Department of Horticulture, Northeast Agricultural University, Changjiang 600, Xiangfang, 150030, Harbin, People’s Republic of China

    Yuanyuan Wang & Xingang Zhou

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  1. Yuanyuan Wang
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  2. Xingang Zhou
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Contributions

ZX conceived and designed the experiment, conducted the experiment, and analyzed the data. WY wrote the paper.

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Correspondence to Xingang Zhou.

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All of the authors of the manuscript entitled “Effects of Green Manures on Perfecting Fungal Community Composition of Rhizosphere Soil of Cucumber Seedlings” declared that we have no conflict of interest to this work. We declare that we do not have any commercial or any other associative interest that represents a conflict of interest in connection with the work submitted. This article does not contain any studies with human participants or animals performed by any of the authors.

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Wang, Y., Zhou, X. Effects of Green Manures on Rhizosphere Fungal Community Composition of Cucumber Seedlings. Curr Microbiol 80, 87 (2023). https://doi.org/10.1007/s00284-023-03199-y

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