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Unraveling the Virulence Factors and Secreted Proteins of an Environmental Isolate Enterobacter sp. S-16

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Abstract

Members of the Enterobacter genus include many pathogenic microbes of humans and plants, secrete proteins that contribute to the interactions of bacteria and their environment. Therefore, understanding of secreted proteins is vital to understand bacterial physiology and behavior. Here, we explored the secretome of an environmental isolate Enterobacter sp. S-16 by nanoLC-MS/MS and identified 572 proteins in the culture supernatant. Gene ontology (GO) analysis indicated that proteins were related to biological processes, molecular as well as cellular functions. The majority of the identified proteins are involved in microbial metabolism, chemotaxis & motility, flagellar hook-associated proteins, biosynthesis of antibiotics, and molecular chaperones to assist the protein folding. Bioinformatics analysis of the secretome revealed the presence of type I and type VI secretion system proteins. Presence of these diverse secretion system proteins in Enterobacter sp. S-16 are likely to be involved in the transport of various proteins including nutrient acquisition, adhesion, colonization, and homeostasis maintenance. Among the secreted bacterial proteins with industrial importance, lignocellulolytic enzymes play a major role, therefore, we analyzed our secretome results for any presence of glycoside hydrolases (GHs) and other hydrolytic enzymes (CAZymes). Overall, the secreted proteins may be considered an attractive reservoir of potential antigens for drug development, diagnostic markers, and other biomedical applications.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

The study was supported by a grant from Department of Biotechnology, Government of India.

Funding

The study was funded by Ramalingswami Re-entry Fellowship, Department of Biotechnology, Government of India (Grant Number BT/RLF 2020-21).

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Authors and Affiliations

  1. Department of Bioengineering and Biotechnology, BIT Mesra, Ranchi, Jharkhand, 835215, India

    Kiran Kumari & Rajnish Prakash Singh

  2. Department of Plant Sciences and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA

    Parva Kumar Sharma

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Contributions

KK and PKS analyzed the secretome data. RPS supervised the work and wrote the original draft.

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Correspondence to Rajnish Prakash Singh.

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Supplementary Information

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284_2023_3197_MOESM1_ESM.docx

Supplementary file1 (DOCX 191 kb)—Neighbor-joining phylogenetic tree based on 16S rRNA sequence. Tree shows phylogenetic relationship between Enterobacter sp. S-16 and other type strains of Enterobacter. Supplementary Fig. 1 Neighbor-joining phylogenetic tree based on 16S rRNA sequence. Tree shows phylogenetic relationship between Enterobacter sp. S-16 and other type strains of Enterobacter. The tree was constructed by using MEGA 7. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test with 1000 replicates

284_2023_3197_MOESM2_ESM.xlsx

Supplementary file2 (XLSX 202 kb)—List of identified secretome proteins (572) have been provided in Supplementary File 2

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Kumari, K., Sharma, P.K. & Singh, R.P. Unraveling the Virulence Factors and Secreted Proteins of an Environmental Isolate Enterobacter sp. S-16. Curr Microbiol 80, 88 (2023). https://doi.org/10.1007/s00284-023-03197-0

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