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Synergism Between Multi-Pseudomonas and Cutinase for Biodegradation of Crude Oil-Based Derivatives

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Abstract

Polyethylene terephthalate (PET) as one of the main crude oil-based derivatives, produces a significant amount of waste that is difficult to degrade. Currently, microbial degradation of PET is an eco-friendly, efficient, and economical method. This study was conducted to propose a novel screening strategy for PET-degrading bacteria, and evaluate their degradation efficiency of PET. Two strains, Pseudomonas nitroreducens S8 and Pseudomonas monteilii S17, were isolated and could utilize PET as a carbon source by co-culture. The combined use of both bacteria gave a synergistic effect on the disruption of the PET surface through colonization behavior, which could enhance the subsequent degradation of PET. Its time of reaching a peak value of PET degradation rate (94.5% at 6 d) was 2 days earlier than these of single bacteria. A similar synergistic effect was also observed in the metabolization of PET monomers, and the metabolic rate was expressed as 82.4% of bis (2-hydroxyethyl) terephthalate (BHET), 64.0% of mono (2-hydroxyethyl) terephthalate (MHET), and 20.0% of terephthalic acid (TPA), respectively. This study is novel in showing the degradation of PET waste by combinations of bacterial pretreatment and enzymatic treatment, which can be a promising method.

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Funding

This work was supported by the Foundation of State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development (Grant No. 33550007–21-ZC0613-0060).

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Authors and Affiliations

  1. State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development, Beijing, China

    Zheng-Fei Yan & Ke-Wei Xu

  2. SINOPEC Key Laboratory of Petroleum Accumulation Mechanisms, Wuxi, China

    Zheng-Fei Yan & Ke-Wei Xu

  3. School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, China

    Zheng-Fei Yan & Jing Wu

Authors
  1. Zheng-Fei Yan
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  2. Ke-Wei Xu
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  3. Jing Wu
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Contributions

ZFY: data curation; writing-original draft; conceived and designed the experiments. KWX: data curation. JW: writing-review & editing.

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Correspondence to Zheng-Fei Yan.

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Yan, ZF., Xu, KW. & Wu, J. Synergism Between Multi-Pseudomonas and Cutinase for Biodegradation of Crude Oil-Based Derivatives. Curr Microbiol 80, 30 (2023). https://doi.org/10.1007/s00284-022-03139-2

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