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Bacterial Diversity Associated with Anastrepha obliqua Males Change Under Mass-Rearing Conditions and with Irradiation

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Abstract

Recent advances in understanding the symbiotic interactions between bacteria and fruit flies have shown that they are relevant for mass rearing and the sterile insect technique (SIT). SIT involves mass production and release of sterile insects that would copulate with their wild conspecifics and thus decrease the population growth rate. The irradiation process used to sterilize mass-reared flies can modify the diversity and structure of the midgut bacterial communities, which could affect sterile male survival, flight capacity, and sexual competitiveness. Our aim was to compare bacterial communities in the midgut of wild and mass-reared Anastrepha obliqua (Macquart) males irradiated at 0, 60, and 80 Gy. After adult’s emergence, their midguts were dissected, DNA was extracted, and high-throughput sequencing of the V3–V4 region of the 16S rDNA gene was performed. A total of 11 phyla, 17 classes, 47 families, and 52 genera of bacteria were identified. The most representative phylum was Proteobacteria and the predominant family was Enterobacteriaceae. We found that wild males had a different intestinal bacterial community from mass-reared males. In addition, irradiation at 60 and 80 Gy caused changes in the diversity and structure of the midgut microbiota of these sterile males, suggesting that mass rearing and irradiation cause artificial selection of the bacterial communities in the gut of A. obliqua males.

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Data Availability

The sequences datasets generated for this study can be found in the GenBank Bioproject PRJNA785925.

Code Availability

Not applicable for that section.

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Acknowledgements

We thank Edouard Jurkevitch for reviewing an earlier draft of this manuscript. We also thank Luis Mario Herrera-Mazon, Damara I. Gómez Olea, Oscar Carmona Castro, Azucena Oropeza Cabrera, Gamaliel Mejía González, Luz Verónica García Fajardo, and Javier Francisco Valle Mora for technical assistance. The Moscafrut facility (SENASICA, SADER) for providing the mass-reared insects and the irradiation facilities.

Funding

This research project was partially funded by the Mexican National Fruit Fly Program/SENASICA/SADER, the National Council for Science and Technology (CONACYT) through project 316135 “Biorational alternatives for the management of fruit flies without agrotoxics, and its interaction with glyphosate residues in the region of Soconusco, Chiapas,” and ECOSUR. It was carried out as a partial requirement for LR-R’s doctoral degree. LR-R was granted a scholarship by CONACYT (CVU 711016).

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Authors and Affiliations

  1. El Colegio de La Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico

    Linnet Roque-Romero, Karina Guillén-Navarro, Eugenia Zarza & Pablo Liedo

  2. Consejo Nacional de Ciencia Y Tecnología (Investigadora Por Mexico), commissioned to El Colegio de La Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico

    Eugenia Zarza

  3. Programa Moscas de La Fruta SENASICA-SADER, Camino a los Cacaotales S/N, C.P. 30860, Metapa de Domínguez, Chiapas, Mexico

    Pablo Montoya

Authors
  1. Linnet Roque-Romero
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  2. Karina Guillén-Navarro
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  3. Eugenia Zarza
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  4. Pablo Montoya
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  5. Pablo Liedo
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Contributions

Experimental design by all authors. Experimental work by LR-R. Molecular and statistical analyses by LR-R and EZ. Preparing first draft LR-R. All authors made contributions in drafting the manuscript, revising it critically for important intellectual content, and approve the submitted version.

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Correspondence to Linnet Roque-Romero.

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Roque-Romero, L., Guillén-Navarro, K., Zarza, E. et al. Bacterial Diversity Associated with Anastrepha obliqua Males Change Under Mass-Rearing Conditions and with Irradiation. Curr Microbiol 80, 26 (2023). https://doi.org/10.1007/s00284-022-03111-0

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