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Characteristic Analysis of Soil-Isolated Bacillus velezensis HY-3479 and Its Antifungal Activity Against Phytopathogens

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Abstract

During the investigation of beneficial agricultural microorganisms, a novel Bacillus strain was isolated. To isolate an effective microorganism that has antifungal activity, soil samples were collected from an agricultural field in the southern area of Pohang, Korea. One strain that had specificity on plant pathogens was analyzed. According to 16S rRNA sequencing, the isolated bacterium was identified as Bacillus velezensis and was designated as HY-3479. Few assays were taken to analyze the characteristics of the HY-3479 strain. In agar plate assay, HY-3479 showed antifungal effects on Colletotrichum acutatum, Cylindrocarpon destructans, Rhizoctonia solani, and Sclerotinia sclerotiorum. The strain also had various enzymatic activities including protease, amylase, and β-1,3-glucanase, which were relatively higher than control strains. Metabolites study of strain HY-3479 was conducted by GC–MS analysis and the bacterium contained many plant growth promoters like 3-methyl-1-butanol, (R, R)-2,3-butanediol, acetoin, and benzoic acid which were not found in untreated TSB medium. In gene expression analysis, antifungal lipopeptide genes like srfc (surfactin) and ituD (iturin A) were highly produced in the HY-3479 strain compared to the control strain KCTC 13417. B. velezensis strain HY-3479 may be the candidate to be an effective microorganism in agriculture and become a beneficial biocontrol agent with plant growth-promoting activities.

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Data Availability

Data used on the present study are available via corresponding author when requested with acceptable reason.

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Acknowledgements

The phytopathogens used in the investigation were distributed by Korean Rural Development Administration for research purposes. The GC–MS analysis was performed with the support of the Kyungpook National University Joint Experiment Lab.

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  1. Department of Advanced Convergence, Handong Global University, Pohang, 37554, South Korea

    Suyoung Song & Eun Kyeong Jeon

  2. Department of Global Leadership School, Handong Global University, Pohang, 37554, South Korea

    Cher-Won Hwang

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  1. Suyoung Song
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Song, S., Jeon, E.K. & Hwang, CW. Characteristic Analysis of Soil-Isolated Bacillus velezensis HY-3479 and Its Antifungal Activity Against Phytopathogens. Curr Microbiol 79, 357 (2022). https://doi.org/10.1007/s00284-022-03060-8

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