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Evaluation of the Active Ingredient of Campsis radicans Essential Oils and its Antimicrobial Evaluation Against Pathogenic Bacteria

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Abstract

Owing to the resistance of nosocomial pathogens to antibiotics, the need for herbal medicines is felt. The aim of this study was to identify the chemical composition of bark essential oils of Campsis radicans and the effect of its free and encapsulated form on resistant nosocomial pathogens. This plant is a native of Northern Iran. The Bark essential oils of Campsis radicans was first extracted and its antimicrobial effects were investigated. Then, its phytochemical compounds were determined using Gas Chromatography−Mass Spectrometry (GC/MS). Guaiacol (2-methoxy phenol) was selected as the active ingredient among 32 compounds (2.40%). It was encapsulated and the encapsulation efficiency (EE), the particle size, polydispersity index (pdi), Fourier transform infrared (FTIR), release, and stability were determined. Then, the antimicrobial effect of both free and encapsulated forms was evaluated on cotrimoxazole-resistant Pseudomonas aeruginosa, cefixime-resistant Escherichia coli, and fluconazole-resistant Candida albicans. It was observed that both free and encapsulated forms of Guaiacol had an antimicrobial effect on the studied resistant strains, but the encapsulated form had a more antimicrobial effect due to more stability and a more targeted effect. MBC (MFC) ranged from 0.270 to 0.439 µg/ml in the free form and from 0.055 to 0.133 µg/ml in the encapsulated form, EE was 86%, particle size, and pdi were 138 nm and 0.26, respectively. This study showed that this plant can be a suitable alternative to chemical drugs due to its antimicrobial effects.

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References

  1. Alizadeh-Behbahani B, Tabatabaei-Yazdi F (2015) In vitro study of antibacterial activity of mangle negro extracts against selected pathogens from enterobacteriaceae and bacillaceae families. Zahedan J Res Med Sci In Press. https://doi.org/10.17795/zjrms-5202

    Article  Google Scholar 

  2. Noshad M, Hojjati M, Alizadeh Behbahani B (2018) Black Zira essential oil: chemical compositions and antimicrobial activity against the growth of some pathogenic strain causing infection. Microb Pathog 116:153–157. https://doi.org/10.1016/j.micpath.2018.01.026

    Article  PubMed  CAS  Google Scholar 

  3. Tabatabaei-Yazdi F, Alizadeh-Behbahani B, Zanganeh H (2015) The Comparison among antibacterial activity of mespilus germanica extracts and number of common therapeutic antibiotics “in vitro.” Zahedan J Res Med Sci In Press. https://doi.org/10.17795/zjrms-5190

    Article  Google Scholar 

  4. Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308–316. https://doi.org/10.1046/j.1365-2672.2000.00969.x

    Article  PubMed  CAS  Google Scholar 

  5. Larypoor M, Frsad S (2011) Evaluation of nosocomial infections in one of hospitals of Qom, 2008. Iran J Med Microbiol 5:7–17

    Google Scholar 

  6. Moein M, Zomorodian K, Almasi M et al (2017) Preparation and analysis of Rosa damascena essential oil composition and antimicrobial activity assessment of related fractions. Iran J Sci Technol Trans A 41:87–94. https://doi.org/10.1007/s40995-017-0220-2

    Article  Google Scholar 

  7. Rossiter SE, Fletcher MH, Wuest WM (2017) Natural products as platforms to overcome antibiotic resistance. Chem Rev 117:12415–12474

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Gherraf N, Zellagui A, Kabouche A et al (2017) Chemical constituents and antimicrobial activity of essential oils of Ammodaucus leucotricus. Arab J Chem 10:S2476–S2478. https://doi.org/10.1016/j.arabjc.2013.09.013

    Article  CAS  Google Scholar 

  9. Siddique S, Parveen Z, Firdaus-e-Bareen MS (2020) Chemical composition, antibacterial and antioxidant activities of essential oils from leaves of three Melaleuca species of Pakistani flora. Arab J Chem 13:67–74. https://doi.org/10.1016/j.arabjc.2017.01.018

    Article  CAS  Google Scholar 

  10. Fazly Bazzaz BS, Khameneh B, Namazi N et al (2018) Solid lipid nanoparticles carrying Eugenia caryophyllata essential oil: the novel nanoparticulate systems with broad-spectrum antimicrobial activity. Lett Appl Microbiol 66:506–513

    Article  PubMed  CAS  Google Scholar 

  11. Khalili ST, Mohsenifar A, Beyki M et al (2015) Encapsulation of Thyme essential oils in chitosan-benzoic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. LWT Food Sci Technol 60:502–508. https://doi.org/10.1016/j.lwt.2014.07.054

    Article  CAS  Google Scholar 

  12. Radünz M, Luiza M, Camargo TM et al (2018) Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum L.) essential oil. Food Chem. https://doi.org/10.1016/j.foodchem.2018.09.173

    Article  PubMed  Google Scholar 

  13. Wen J, Jansen RK (1995) Morphological and molecular comparisons of Campsis grandiflora and C. radicans (Bignoniaceae), an eastern Asian and eastern North American vicariad species pair. Plant Syst Evol 196:173–183. https://doi.org/10.1007/BF00982958

    Article  Google Scholar 

  14. Islam M, Jannat T, Kuddus MR et al (2019) In vitro and in vivo evaluation of pharmacological potentials of Campsis radicans L. Clin Phytosci. https://doi.org/10.1186/s40816-019-0144-9

    Article  Google Scholar 

  15. Panda SP, Panigrahy UP, Panda S, Jena BR (2019) Stem extract of Tabebuia chrysantha induces apoptosis by targeting sEGFR in Ehrlich Ascites Carcinoma. J Ethnopharmacol 235:219–226

    Article  PubMed  CAS  Google Scholar 

  16. Sobiyana P, Anburaj G, Manikandan R (2019) Comparative analysis of the in vitro antioxidant antivity of Tabebuia rosea. and Tabebuia argentea. J Pharmacogn Phytochem 8:2673–2677

    CAS  Google Scholar 

  17. Mohammadi S, Sharifi-rad J, Asgharian P, Martorell M (2020) Medicinal plants used in the treatment of malaria: a key emphasis to Artemisia. Cinchona, Cryptolepis, and Tabebuia Genera. https://doi.org/10.1002/ptr.6628

    Article  Google Scholar 

  18. Kiarsi Z, Hojjati M, Behbahani BA, Noshad M (2020) In vitro antimicrobial effects of Myristica fragrans essential oil on foodborne pathogens and its influence on beef quality during refrigerated storage. J Food Saf. https://doi.org/10.1111/jfs.12782

    Article  Google Scholar 

  19. Beladi M, Akhavansepahy A, Mehrabian S, Esmaili A, Sharifnia F (2020) Evaluation of antimicrobial role of nanocapsules containing Cornus mass extract synthesized by emulsion method on antibiotic resistant bacteria. J Med Plants 19:84–107. https://doi.org/10.29252/jmp.19.74.84

    Article  Google Scholar 

  20. Liu C, Liang B, Shi G et al (2015) Preparation and characteristics of nanocapsules containing essential oil for textile application. Flavour Fragr J 30:295–301. https://doi.org/10.1002/ffj.3245

    Article  CAS  Google Scholar 

  21. Sorourian R, Eghbal A, Mehrnoosh K et al (2022) Structural characterization and cytotoxic, ACE—inhibitory and antioxidant activities of polysaccharide from Bitter vetch (Vicia ervilia ) seeds. J Food Measure Charact. https://doi.org/10.1007/s11694-022-01512-0

    Article  Google Scholar 

  22. Sebaaly C, Charcosset C, Stainmesse S et al (2016) Clove essential oil-in-cyclodextrin-in-liposomes in the aqueous andlyophilized states: from laboratory to large scale using a membranecontactor. Carbohyd Polym 138:75–85. https://doi.org/10.1016/j.carbpol.2015.11.053

    Article  CAS  Google Scholar 

  23. Shetta A, Kegere J, Mamdouh W (2018) Comparative study of encapsulated peppermint and green tea essential oils in chitosan nanoparticles: encapsulation, thermal stability, in-vitro release, antioxidant and antibacterial activities. Int J of Biol Macromol 126:731–742. https://doi.org/10.1016/j.ijbiomac.2018.12.161

    Article  CAS  Google Scholar 

  24. Sayout A, Ouarhach A, Dilagui I et al (2019) Antibacterial activity and chemical composition of essential oil from Lavandula tenuisecta Coss.ex Ball. an endemic species from Morocco. Eur J Integr Med 33:101017. https://doi.org/10.1016/j.eujim.2019.101017

    Article  Google Scholar 

  25. Bhattacharjee I, Ghosh A, Chandra G (2005) Antimicrobial activity of the essential oil of Cestrum diurnum (L.) (Solanales: Solanaceae). Afr J Biotech 4:371–374. https://doi.org/10.4314/ajb.v4i4.15109

    Article  CAS  Google Scholar 

  26. Anthony SJ, Zuchowski W, Setzer WN (2009) Composition of the floral essential oil of Brugmansia suaveolens. Rec Nat Prod 3(2):76

    CAS  Google Scholar 

  27. Snoussi M, Noumi E, Punchappady-Devasya R et al (2018) Antioxidant properties and anti-quorum sensing potential of Carum copticum essential oil and phenolics against Chromobacterium violaceum. J Food Sci Technol 55:2824–2832. https://doi.org/10.1007/s13197-018-3219-6

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  28. Esmaeili A, Saremnia B (2012) Preparation of extract-loaded nanocapsules from Onopordon leptolepis DC. Ind Crops Prod 37:259–263. https://doi.org/10.1016/j.indcrop.2011.12.010

    Article  CAS  Google Scholar 

  29. Li Y, Kong D, Lin X et al (2016) Quality evaluation for essential oil of cinnamomum verum leaves at different growth stages based on GC–MS. FTIR and Microsc. https://doi.org/10.1007/s12161-015-0187-6

    Article  Google Scholar 

  30. Souguir H, Salaün F, Douillet P et al (2013) Nanoencapsulation of curcumin in polyurethane and polyurea shells by an emulsion diffusion method. Chem Eng J 221:133–145. https://doi.org/10.1016/j.cej.2013.01.069

    Article  CAS  Google Scholar 

  31. Ciko L, Andoni A, Ylli F et al (2016) Extraction of essential oil from albanian Salvia officinalis L. and its characterization by FTIR spectroscopy. Asian J Chem 28:1401–1402. https://doi.org/10.14233/ajchem.2016.19658

    Article  CAS  Google Scholar 

  32. Yakhchi V, Jahanizadeh S, Yazdian F et al (2020) Synthesis and evaluation of lipid-based nanoparticle containing ginger extract against aspergillus species. J Shahid Sadoughi Univ Med Sci. https://doi.org/10.18502/ssu.v28i6.4155

    Article  Google Scholar 

  33. Akbarzadeh I, Shayan M, Bourbour M et al (2021) Preparation, optimization and in-vitro evaluation of curcumin-loaded niosome@ calcium alginate nanocarrier as a new approach for breast cancer treatment. Biology 10:173

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  34. Vishwakarma GS, Gautam N, Babu JN, Jaitak V (2016) Polymeric encapsulates of essential oils and their constituents : a review of preparation techniques, characterization, and sustainable release mechanisms polymeric encapsulates of essential oils and their constituents. Polym Rev 56:668–701. https://doi.org/10.1080/15583724.2015.1123725

    Article  CAS  Google Scholar 

  35. Jobdeedamrong A, Jenjob R, Crespy D, Accepted J (2018). Encapsulation and release of essential oils in functional silica nanocontainers. https://doi.org/10.1021/acs.langmuir.8b01652

    Article  Google Scholar 

  36. Mahmoudzadeh M, Hosseini H, Shahraz F et al (2017) Essential oil composition and antioxidant capacity of carum copticum and its antibacterial effect on Staphylococcus aureus, Enterococcus faecalis and Escherichia coli O157:H7. J Food Process Preserv 41:1–11. https://doi.org/10.1111/jfpp.12938

    Article  CAS  Google Scholar 

  37. Rakmai J, Cheirsilp B, Carlos J et al (2018) Antioxidant and antimicrobial properties of encapsulated guava leaf oil in hydroxypropyl-beta-cyclodextrin. Ind Crops Prod 111:219–225. https://doi.org/10.1016/j.indcrop.2017.10.027

    Article  CAS  Google Scholar 

  38. Essien EE, Ogunwande IA, Setzer WN, Ekundayo O (2012) Chemical composition, antimicrobial, and cytotoxicity studies on S. erianthum and S. macranthum essential oils. Pharm Biol 50:474–480. https://doi.org/10.3109/13880209.2011.614623

    Article  PubMed  CAS  Google Scholar 

  39. Ahmadi Roudbaraki Z, Ranji N, Soltani Tehrani B (2017) Deregulation of mexb gene in ciprofloxacin resistant isolates of pseudomonas aeruginosa treated with silibinin-encapsulated in nanoparticles. J Babol Univ Med Sci 19:42–49

    Google Scholar 

  40. Ghomi Z, Tafvizi F, Naseh V, Akbarzadeh I (2020) Effect of Artemisia ciniformis extract on expression of NorA efflux pump gene in ciprofloxacin resistant Staphylococcus aureus by real time PCR. Iran J Med Microbiol 14:55–69

    Article  Google Scholar 

  41. Rahimzadeh-Torabi L, Doudi M, Naghsh N, Golshani Z (2016) Comparing the antifungal effects of gold and silver nanoparticles isolated from patients with vulvovaginal candidiasis in-vitro. KAUMS J (FEYZ) 20:331–339

    Google Scholar 

  42. Ghaderi RS, Kazemi M, Soleimanpour S (2021) Nanoparticles are more successful competitor than antibiotics in treating bacterial infections: a review of the literature. Iran J Med Microbiol 15:18–45

    Article  Google Scholar 

  43. Rashidian S, Mirzaie A et al (2018) Antibacterial and anticancer activities of biosynthesized silver nanoparticles using Artemisia khorassanica extract: Bax and Bcl2 apoptosis gene expression analysis. SSU J 26:1039–1049

    Google Scholar 

  44. Du SS, Yang K, Wang CF et al (2014) Chemical constituents and activities of the essential oil from myristica fragrans against cigarette beetle lasioderma serricorne. Chem Biodivers 11:1449–1456. https://doi.org/10.1002/cbdv.201400137

    Article  PubMed  CAS  Google Scholar 

  45. Van NH, Meile J-C, Lebrun M et al (2018) Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity. Lett Appl Microbiol 66:207–214

    Article  Google Scholar 

  46. Esmaeili A, Rahnamoun S, Sharifnia F (2013) Effect of O/W process parameters on Crataegus azarolus L nanocapsule properties. J Nanobiotechnol 11:1–9. https://doi.org/10.1186/1477-3155-11-16

    Article  CAS  Google Scholar 

  47. Kalhor N, Mirzaie A, Hamdi SMM (2021) Anti-biofilm effects of zinc oxide nanoparticles synthesized by leaf extract of typha latifolia on biofilm gene expression in multidrug-resistant klebsiella pneumoniae strains: a laboratory study. J Rafsanjan Univ Med Sci 20:3–22

    Google Scholar 

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Acknowledgements

The authors of this article express their gratitude for receiving technical assistance from the Islamic Azad University, North Tehran Branch.

Funding

The authors received no financial support for the research, authorship, and/or publication of this article.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran

    Maryam Ramtin & Mohaddeseh Larypoor

  2. Department of Biology, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran

    Fariba Sharifniya

  3. Department of Microbiology, Faculty of Biological Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Mirsasan Mirpour

  4. Department of Biochemistry, Faculty of Biological Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Saeid Zarrabi

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  2. Fariba Sharifniya
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Contributions

MR: Investigation (equal), Methodology (equal), Writing-original draft (equal); FS: Project administration (equal), Supervision (equal); ML: Supervision (equal), Writing-review & editing (equal); MM: Conceptualization (equal), Data curation (equal); SZ: Conceptualization (equal), Data curation (equal).

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Correspondence to Mohaddeseh Larypoor.

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Ramtin, M., Sharifniya, F., Larypoor, M. et al. Evaluation of the Active Ingredient of Campsis radicans Essential Oils and its Antimicrobial Evaluation Against Pathogenic Bacteria. Curr Microbiol 79, 338 (2022). https://doi.org/10.1007/s00284-022-03042-w

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