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Bioactive Metabolites of Lactiplantibacillus plantarum K014 Against Methicillin-Resistant Staphylococcus aureus ATCC43300 and In Vitro Evaluation of Its Antibacterial, Antioxidant and Anti-inflammatory Activities

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Abstract

This study aims to evaluate the effects of bioactive metabolites produced by lactic acid bacteria against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300. A total of six lactic acid bacteria (LAB) were selected to evaluate the antimicrobial activity against MRSA ATCC 43300, a skin pathogen that is highly resistant to most antibiotics. The K014 isolate from a fermented vegetable recorded the highest inhibition against MRSA ATCC 43300 at 91.93 ± 0.36%. 16S rRNA sequencing revealed the K014 isolate is closely related to L. plantarum and the sequence was subsequently deposited in the GenBank database with an accession number of MW180960, named as Lactiplantibacillus plantarum K014. The cell-free supernatant (CFS) of L. plantarum K014 had tolerance to high temperature as well as acidic pH. The bioactive metabolites, such as hydrogen peroxide, lactic acid and hyaluronic acid, were produced by L. plantarum K014. Result from ABTS assay showed higher antioxidant activity (46.28%) as compared to that obtained by DPPH assay (2.97%). The CFS had showed anti-inflammatory activity for lipoxygenase (LOX) assay at 43.66%. The bioactive metabolites of L. plantarum K014 showed very promising potential to be used topical skin pathogens.

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Acknowledgements

The authors acknowledge the financial support received by Ministry of Higher Education Malaysia for Fundamental Research Grant Scheme with Project Code: FRGS/1/2022/STG01/USM/02/21.

Funding

This study was supported by Fundamental Research Grant Scheme (FRGS) by Ministry of Higher Education, Malaysia (Project Code: FRGS/1/2018/SKK11/USM/02/1).

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Authors and Affiliations

  1. Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia

    Larry Kurai Mlambo, Hock Wei Tang, Zhang Jin Ng & Joo Shun Tan

  2. Halal Products Research Institute, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Sahar Abbasiliasi

  3. School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia

    Thaigarajan Parumasivam

  4. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia

    Khayriyyah Mohd Hanafiah & Aemen Ali Kudayr Al-Shammary

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  1. Larry Kurai Mlambo
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Contributions

LKM conducted the experiments and wrote the manuscript. HWT and ZJN conducted the experiments and analyse the data. TP, KMH and AAKAS provided the facility to conduct the MRSA experiment. SA and JST provided experiment idea, revised the manuscript and supervised the research.

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Correspondence to Joo Shun Tan.

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Lactiplantibacillus plantarum strain K014 16S ribosomal RNA gene, partial sequence MW180960.

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Mlambo, L.K., Abbasiliasi, S., Tang, H.W. et al. Bioactive Metabolites of Lactiplantibacillus plantarum K014 Against Methicillin-Resistant Staphylococcus aureus ATCC43300 and In Vitro Evaluation of Its Antibacterial, Antioxidant and Anti-inflammatory Activities. Curr Microbiol 79, 359 (2022). https://doi.org/10.1007/s00284-022-03038-6

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