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Streptomyces: Derived Active Extract Inhibits Candida albicans Biofilm Formation

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Abstract

Candida albicans is an opportunistic pathogen that causes biofilm-associated infections. C. albicans biofilms are known to display reduced susceptibility to antimicrobials and high rates of acquired antibiotic resistance, and biofilm forming in C. albicans further hampers treatment options and highlights the need for new antibiofilm strategies. Identifying active components from desert actinomycetes strains to inhibit the formation of C. albicans biofilms represents an effective treatment strategy. In this study, actinomycetes that can inhibit C. albicans biofilm formation were isolated from the Taklimakan Desert, and the underlying mechanisms were explored. After screening the anti-C.albicans biofilm activities of culture supernatants from 170 Actinomycete strains, six strains showed significant inhibition of C. albicans biofilm formation. Microscopic examination showed a reduction in biofilm formation of C. albicans treated with supernatants from actinomycetes. Scanning electron microscopy showed that the morphological changes in biofilm cells were caused by cell membrane rupture and cell material leakage. Then, C.albicans biofilms were destroyed by changing the content of extracellular polysaccharides or degrading extracellular DNA. Finally, a preliminary study on active substances extracted from a new species (TRM43335) showed that the substances that inhibited the formation of biofilms might be peptides. This study provides preliminary evidence that desert actinomyces strains have inhibitory effects on the biofilm development of C. albicans.

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Acknowledgements

This paper was funded by the Guangxi Natural Science Foundation. Grant Number (Nos. AD22035111, yy 2021sk001) and professional English editing company.

Funding

This paper was funded by Guangxi Natural Science Foundation. Grant Number [No. AD22035111].

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  1. Sheng-qiang Yang and Hui-jie Zhou have contributed equally to this work.

  2. Li-ping Teng and Hong Zeng have contributed equally to this work.

Authors and Affiliations

  1. School of Basic Medicine, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, People’s Republic of China

    Sheng-qiang Yang, Li-ping Teng & Hong Zeng

  2. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, College of Life Science, Tarim University, Alar, China

    Hui-jie Zhou

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Contributions

All authors contributed to the study conception and design. The oversight and leadership responsibilities of the research planning and execution, including mentorship external to the core team, were performed by LT and HZ. The overall replication/reproduction of the experiments and other research results were performed by SY. The first draft of the manuscript was written by SY, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Li-ping Teng or Hong Zeng.

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Yang, Sq., Zhou, Hj., Teng, Lp. et al. Streptomyces: Derived Active Extract Inhibits Candida albicans Biofilm Formation. Curr Microbiol 79, 332 (2022). https://doi.org/10.1007/s00284-022-03013-1

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