Skip to main content

Advertisement

SpringerLink
Log in

Exploring the Oxidative Stress Regulation of Mice with Hyperglycemia by Lactiplantibacillus plantarum SCS4

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate the effects of Lactiplantibacillus plantarum SCS4 (L. plantarum SCS4) on oxidative stress in streptozocin-induced hyperglycemic mice. After establishment of the hyperglycemic model, control group mice were gavaged daily with phosphate-buffered saline, while different experimental groups (AG, BG, and CG) mice were gavaged with L. plantarum SCS4 suspension, cellular inclusion suspension, and inactivated inclusion suspension for 10 weeks, respectively. Compared with the model group (MG) group, the results showed that fasting blood glucose levels in BG and CG groups decreased, and postprandial 2-h blood glucose levels in BG groups decreased, whereas glucose tolerance improved. Meanwhile, ROS and MDA levels in serum of AG mice were decreased significantly (P < 0.05). Compared with the MG group, serum levels of GPx, HO-1, and NQO1 were increased in the BG group, whereas serum levels of CAT, HO-1, and GSH were increased in the CG group. Our results indicate that L. plantarum SCS4 can alleviate oxidative stress induced by hyperglycemia, and there may be synergistic effects among the different treatments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data Availability

The raw and processed data required to reproduce these findings cannot be shared at this time as the data also forms part of an ongoing study.

Code Availability

Not applicable.

References

  1. Zhao JC (2018) Evaluation of antioxidative abilities of Lactobacillus plantarum and the antioxidative mechanisms. Dissertation, Jiangnan University

  2. Wiernsperger NF (2003) Oxidative stress: the special case of diabetes. BioFactors 19(1–2):11–18. https://doi.org/10.1002/biof.5520190103

    Article  PubMed  CAS  Google Scholar 

  3. Dehdashtian E, Mehrzadi S, Yousefi B, Hosseinzadehm A, Reiter RJ, Safa M et al (2018) Diabetic retinopathy pathogenesis and the ameliorating effects of melatonin; involvement of autophagy, inflammation and oxidative stress. Life Sci 193:20–33. https://doi.org/10.1016/j.lfs.2017.12.001

    Article  PubMed  CAS  Google Scholar 

  4. Salminen S, Von Wright A (2004) Lactic acid bacteria: microbiological and functional aspects. CRC Press, Boca Raton

    Book  Google Scholar 

  5. Toral M, Gómez-Guzmán M, Jiménez R, Romero M, Sánchez M, Utrilla MP et al (2014) The probiotic Lactobacillus coryniformis CECT5711 reduces the vascular pro-oxidant and pro-inflammatory status in obese mice. Clin Sci 127(1):33–45. https://doi.org/10.1042/CS20130339

    Article  Google Scholar 

  6. Persichetti E, De Michele A, Codini M, Traina G (2014) Antioxidative capacity of Lactobacillus fermentum LF31 evaluated in vitro by oxygen radical absorbance capacity assay. Nutrition 30(7–8):936–938. https://doi.org/10.1016/j.nut.2013.12.009

    Article  PubMed  CAS  Google Scholar 

  7. Li C, Ding Q, Nie SP, Zhang YS, Xiong T, Xie MY (2014) Carrot juice fermented with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats. J Agric Food Chem 62(49):11884–11891. https://doi.org/10.1021/jf503681r

    Article  PubMed  CAS  Google Scholar 

  8. Meng X, Chen CL, Sun JY, Jing L, Zuo LL, Wu LJ (2022) Alleviation of oxidative stress in pancreatic tissue of hyperglycemic mice by Lactiplantibacillus plantarum SCS4. J Food Biochem. https://doi.org/10.1111/jfbc.14256

    Article  PubMed  Google Scholar 

  9. GB 4789.35–2016 (2016) National Standard for Food Safety (Food Microbiology Test Lactic acid bacteria test). National Health Commission of the People's Republic of China, State Administration for Market Regulation Beijing

  10. Wang YN, Li MG, Ni ZZ (2018) Primary study on the hypoglycemic mechanism of 5rolGLP-HV in STZ-induced type 2 diabetes mellitus mice. J Biosci 43(5):921–929. https://doi.org/10.1007/s12038-018-9809-7

    Article  PubMed  CAS  Google Scholar 

  11. DeFronzo RA, Ferrannini E, Groop L, Henry RR, Herman WH, Holst JJ et al (2015) Type 2 diabetes mellitus. Nat Rev Dis Primers 1(1):1–22. https://doi.org/10.1038/nrdp.2015.19

    Article  Google Scholar 

  12. Ha H, Kim KH (1999) Pathogenesis of diabetic nephropathy: the role of oxidative stress and protein kinase C. Diabetes Res Clin Pract 45(2–3):147–151. https://doi.org/10.1016/S0168-8227(99)00044-3

    Article  PubMed  CAS  Google Scholar 

  13. Ugochukwu NH, Cobourne MK (2003) Modification of renal oxidative stress and lipid peroxidation in streptozotocin-induced diabetic rats treated with extracts from Gongronema latifolium leaves. Clin Chim Acta 336(1–2):73–81. https://doi.org/10.1016/S0009-8981(03)00325-5

    Article  PubMed  CAS  Google Scholar 

  14. Shaw JE, Sicree RA, Zimmet PZ (2010) Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 87(1):4–14. https://doi.org/10.1016/j.diabres.2009.10.007

    Article  PubMed  CAS  Google Scholar 

  15. Bejar W, Hamden K, Ben Salah R, Chouayekh H (2013) Lactobacillus plantarum TN627 significantly reduces complications of alloxan-induced diabetes in rats. Anaerobe 24:4–11. https://doi.org/10.1016/j.anaerobe.2013.08.006

    Article  PubMed  CAS  Google Scholar 

  16. Yang FY, Wang JY, Zhang HX, Xie YH, Jin JH, Liu H et al (2021) Hypoglycemic effects of space-induced Lactobacillus plantarum SS18-5 on type 2 diabetes in a rat model. J Food Biochem 45(9):e13899. https://doi.org/10.1111/jfbc.13899

    Article  PubMed  CAS  Google Scholar 

  17. Huang HY, Korivi M, Tsai CH, Yang JH, Tsai YC (2013) Supplementation of Lactobacillus plantarum K68 and fruit-vegetable ferment along with high fat-fructose diet attenuates metabolic syndrome in rats with insulin resistance. Evidence-Based Complement Altern Med. https://doi.org/10.1155/2013/943020

    Article  Google Scholar 

  18. Fu JQ, Hou YY, Xue P, Wang HH, Xu YY, Qu WD et al (2016) Nrf2 in type 2 diabetes and diabetic complications: yin and yang. Curr Opin Toxicol 1:9–19. https://doi.org/10.1016/j.cotox.2016.08.001

    Article  Google Scholar 

  19. Sarvestani NN, Firouzi SS, Falak R, Karimi MY, Gholami MD, Rangbar A, Hosseini A (2018) Phosphodiesterase 4 and 7 inhibitors produce protective effects against high glucose-induced neurotoxicity in PC12 cells via modulation of the oxidative stress, apoptosis and inflammation pathways. Metabol Brain Dis 33(4):1293–1306. https://doi.org/10.1007/s11011-018-0241-3

    Article  CAS  Google Scholar 

  20. Kaneto H, Kajimoto Y, Fujitani Y, Matsuoka T, Sakamoto K, Matsuhisa M (1999) Oxidative stress induces p21 expression in pancreatic islet cells: possible implication in beta-cell dysfunction. Diabetologia 42:1093–1097. https://doi.org/10.1007/s001250051276

    Article  PubMed  CAS  Google Scholar 

  21. Sakai K, Matsumoto K, Nishikawa T, Suefuji M, Nakamaru K, Hirashima Y (2003) Mitochondrial reactive oxygen species reduce insulin secretion by pancreatic β-cells. Biochem Biophys Res Commun 300:216–222. https://doi.org/10.1016/S0006-291X(02)02832-2

    Article  PubMed  CAS  Google Scholar 

  22. Lykkesfeldt J (2007) Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clin Chim Acta 380:50–58. https://doi.org/10.1016/j.cca.2007.01.028

    Article  PubMed  CAS  Google Scholar 

  23. Victor RP (2014) Diabetes-oxidative stress and dietary antioxidants. Academic Press, London

    Google Scholar 

  24. Zeng Z, Yuan QP, Yu R, Zhang JL, Ma HQ, Chen SW (2019) Ameliorative effects of probiotic lactobacillus paracasei NL41 on insulin sensitivity, oxidative stress, and beta-cell function in a type 2 diabetes mellitus rat model. Mol Nutr Food Res 63(22):1900457. https://doi.org/10.1002/mnfr.201900457

    Article  CAS  Google Scholar 

  25. Ben Salah-Abbès J, Mannai M, Belgacem H, Zinedine A, Abbès S (2021) Efficacy of lactic acid bacteria supplementation against Fusarium graminearum growth in vitro and inhibition of Zearalenone causing inflammation and oxidative stress in vivo. Toxicon 202:115–122. https://doi.org/10.1016/j.toxicon.2021.09.010

    Article  PubMed  CAS  Google Scholar 

  26. Ge QF, Yang B, Liu R, Jiang DL, Yu H, Wu MG, Zhang WG (2021) Antioxidant activity of Lactobacillus plantarum NJAU-01 in an animal model of aging. BMC Microbiol 21(1):182. https://doi.org/10.1186/s12866-021-02248-5

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Chen W, Tian FW, Zhao X, Zhao N (2012) Recent advance on intervention of lab in oxidative stress. J Chin Inst Food Sci Technol 12(11):1–7. https://doi.org/10.16429/j.1009-7848.2012.11.001

    Article  Google Scholar 

  28. Cuevas-González PF, Aguilar-Toalá JE, García HS, González-Córdova AF, Vallejo-Cordoba B, Hernández-Mendoza A (2020) Protective effect of the intracellular content from potential probiotic bacteria against oxidative damage induced by acrylamide in human erythrocytes. Probiot Antimicrob Proteins 12:1459–1470. https://doi.org/10.1007/s12602-020-09636-9

    Article  CAS  Google Scholar 

  29. Aguilar-Toalá JE, Estrada-Montoya MC, Liceaga AM, Garcia HS, González-Aguilar GA, Vallejo-Cordoba B (2019) An insight on antioxidant properties of the intracellular content of Lactobacillus casei CRL-431. LWT 102:58–63. https://doi.org/10.1016/j.lwt.2018.12.015

    Article  CAS  Google Scholar 

  30. Aguilar-Toalá JE, Hall FG, Urbizo-Reyes UC, Garcia HS, Vallejo-Cordoba B, González-Córdova AF et al (2020) In silico prediction and in vitro assessment of multifunctional properties of postbiotics obtained from two probiotic bacteria. Probiot Antimicrob Proteins 12(2):608–622. https://doi.org/10.1007/s12602-019-09568-z

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Thanks to Fund Sichuan Science and Technology Program in China Grant/Award No. 2019YJ0494 and A Project Supported by Scientific Research Fund of Sichuan Provincial Education Department in China Grant/Award No. 18ZA1075 for their support of this article.

Funding

This study was supported by Sichuan Science and Technology Program in China Grant/Award No. 2019YJ0494 and A Project Supported by Scientific Research Fund of Sichuan Provincial Education Department in China Grant/Award No. 18ZA1075.

Author information

Author notes

  1. Xiao Meng and Xin-Zhi Chen have contributed equally to the work.

Authors and Affiliations

  1. Institute of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China

    Xiao Meng, Xin-Zhi Chen, Ying Zhang, Li-Shi Jiang & Juan Wang

  2. Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China

    Jia-Yi Sun

Authors
  1. Xiao Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin-Zhi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jia-Yi Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ying Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li-Shi Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

XM was responsible for the experimental design. XM and X-ZC were responsible for the experimental implementation and data processing. J-yS and YZ are responsible for experimental process instrumentation and quality control. L-SJ and JW are responsible for experimental data processing and paper quality control.

Corresponding authors

Correspondence to Li-Shi Jiang or Juan Wang.

Ethics declarations

Conflict of interest

The authors of this manuscript state that they do not have conflict of interest to declare.

Ethical Approval

The study was approved by the Animal Ethics Committee of Chengdu University of Traditional Chinese Medicine (2019–21) and conducted in compliance with Directive 2010/63/EU (European Parliament and Council of the European Union, 2010).

Consent to Participate

Not applicable.

Consent to Publish

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Meng, X., Chen, XZ., Sun, JY. et al. Exploring the Oxidative Stress Regulation of Mice with Hyperglycemia by Lactiplantibacillus plantarum SCS4. Curr Microbiol 79, 319 (2022). https://doi.org/10.1007/s00284-022-03008-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00284-022-03008-y

Search

Navigation